Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.

Tsetse flies use olfactory and gustatory responses, through odorant and gustatory receptors (ORs and GRs), to interact with their environment. Glossina morsitans morsitans genome ORs and GRs were annotated using homologs of these genes in Drosophila melanogaster and an ab initio approach based on OR...

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Autores principales:	George F O Obiero, Paul O Mireji, Steven R G Nyanjom, Alan Christoffels, Hugh M Robertson, Daniel K Masiga
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/73fa1b5784d144558e8693554ef716ee
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spelling	oai:doaj.org-article:73fa1b5784d144558e8693554ef716ee2021-11-18T09:16:09ZOdorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.1935-27271935-273510.1371/journal.pntd.0002663https://doaj.org/article/73fa1b5784d144558e8693554ef716ee2014-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24763191/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Tsetse flies use olfactory and gustatory responses, through odorant and gustatory receptors (ORs and GRs), to interact with their environment. Glossina morsitans morsitans genome ORs and GRs were annotated using homologs of these genes in Drosophila melanogaster and an ab initio approach based on OR and GR specific motifs in G. m. morsitans gene models coupled to gene ontology (GO). Phylogenetic relationships among the ORs or GRs and the homologs were determined using Maximum Likelihood estimates. Relative expression levels among the G. m. morsitans ORs or GRs were established using RNA-seq data derived from adult female fly. Overall, 46 and 14 putative G. m. morsitans ORs and GRs respectively were recovered. These were reduced by 12 and 59 ORs and GRs respectively compared to D. melanogaster. Six of the ORs were homologous to a single D. melanogaster OR (DmOr67d) associated with mating deterrence in females. Sweet taste GRs, present in all the other Diptera, were not recovered in G. m. morsitans. The GRs associated with detection of CO2 were conserved in G. m. morsitans relative to D. melanogaster. RNA-sequence data analysis revealed expression of GmmOR15 locus represented over 90% of expression profiles for the ORs. The G. m. morsitans ORs or GRs were phylogenetically closer to those in D. melanogaster than to other insects assessed. We found the chemoreceptor repertoire in G. m. morsitans smaller than other Diptera, and we postulate that this may be related to the restricted diet of blood-meal for both sexes of tsetse flies. However, the clade of some specific receptors has been expanded, indicative of their potential importance in chemoreception in the tsetse.George F O ObieroPaul O MirejiSteven R G NyanjomAlan ChristoffelsHugh M RobertsonDaniel K MasigaPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 8, Iss 4, p e2663 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 George F O Obiero Paul O Mireji Steven R G Nyanjom Alan Christoffels Hugh M Robertson Daniel K Masiga Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.
description	Tsetse flies use olfactory and gustatory responses, through odorant and gustatory receptors (ORs and GRs), to interact with their environment. Glossina morsitans morsitans genome ORs and GRs were annotated using homologs of these genes in Drosophila melanogaster and an ab initio approach based on OR and GR specific motifs in G. m. morsitans gene models coupled to gene ontology (GO). Phylogenetic relationships among the ORs or GRs and the homologs were determined using Maximum Likelihood estimates. Relative expression levels among the G. m. morsitans ORs or GRs were established using RNA-seq data derived from adult female fly. Overall, 46 and 14 putative G. m. morsitans ORs and GRs respectively were recovered. These were reduced by 12 and 59 ORs and GRs respectively compared to D. melanogaster. Six of the ORs were homologous to a single D. melanogaster OR (DmOr67d) associated with mating deterrence in females. Sweet taste GRs, present in all the other Diptera, were not recovered in G. m. morsitans. The GRs associated with detection of CO2 were conserved in G. m. morsitans relative to D. melanogaster. RNA-sequence data analysis revealed expression of GmmOR15 locus represented over 90% of expression profiles for the ORs. The G. m. morsitans ORs or GRs were phylogenetically closer to those in D. melanogaster than to other insects assessed. We found the chemoreceptor repertoire in G. m. morsitans smaller than other Diptera, and we postulate that this may be related to the restricted diet of blood-meal for both sexes of tsetse flies. However, the clade of some specific receptors has been expanded, indicative of their potential importance in chemoreception in the tsetse.
format	article
author	George F O Obiero Paul O Mireji Steven R G Nyanjom Alan Christoffels Hugh M Robertson Daniel K Masiga
author_facet	George F O Obiero Paul O Mireji Steven R G Nyanjom Alan Christoffels Hugh M Robertson Daniel K Masiga
author_sort	George F O Obiero
title	Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.
title_short	Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.
title_full	Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.
title_fullStr	Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.
title_full_unstemmed	Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.
title_sort	odorant and gustatory receptors in the tsetse fly glossina morsitans morsitans.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/73fa1b5784d144558e8693554ef716ee
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Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans.

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