Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro

Abstract Plant-derived extracellular vesicles (EVs) gain more and more attention as promising carriers of exogenous bioactive molecules to the human cells. Derived from various edible sources, these EVs are remarkably biocompatible, biodegradable and highly abundant from plants. In this work, EVs fr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luiza Garaeva, Roman Kamyshinsky, Yury Kil, Elena Varfolomeeva, Nikolai Verlov, Elena Komarova, Yuri Garmay, Sergey Landa, Vladimir Burdakov, Alexander Myasnikov, Ilya A. Vinnikov, Boris Margulis, Irina Guzhova, Alexander Kagansky, Andrey L. Konevega, Tatiana Shtam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/73fa49eb7021498780117d600e00ceee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:73fa49eb7021498780117d600e00ceee
record_format	dspace
spelling	oai:doaj.org-article:73fa49eb7021498780117d600e00ceee2021-12-02T13:24:26ZDelivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro10.1038/s41598-021-85833-y2045-2322https://doaj.org/article/73fa49eb7021498780117d600e00ceee2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85833-yhttps://doaj.org/toc/2045-2322Abstract Plant-derived extracellular vesicles (EVs) gain more and more attention as promising carriers of exogenous bioactive molecules to the human cells. Derived from various edible sources, these EVs are remarkably biocompatible, biodegradable and highly abundant from plants. In this work, EVs from grapefruit juice were isolated by differential centrifugation followed by characterization of their size, quantity and morphology by nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy and cryo-electron microscopy (Cryo-EM). In Cryo-EM experiments, we visualized grapefruit EVs with the average size of 41 ± 13 nm, confirmed their round-shaped morphology and estimated the thickness of their lipid bilayer as 5.3 ± 0.8 nm. Further, using cell culture models, we have successfully demonstrated that native grapefruit-derived extracellular vesicles (GF-EVs) are highly efficient carriers for the delivery of the exogenous Alexa Fluor 647 labeled bovine serum albumin (BSA) and heat shock protein 70 (HSP70) into both human peripheral blood mononuclear cells and colon cancer cells. Interestingly, loading to plant EVs significantly ameliorated the uptake of exogenous proteins by human cells compared to the same proteins without EVs. Most importantly, we have confirmed the functional activity of human recombinant HSP70 in the colon cancer cell culture upon delivery by GF-EVs. Analysis of the biodistribution of GF-EVs loaded with 125I-labeled BSA in mice demonstrated a significant uptake of the grapefruit-derived extracellular vesicles by the majority of organs. The results of our study indicate that native plant EVs might be safe and effective carriers of exogenous proteins into human cells.Luiza GaraevaRoman KamyshinskyYury KilElena VarfolomeevaNikolai VerlovElena KomarovaYuri GarmaySergey LandaVladimir BurdakovAlexander MyasnikovIlya A. VinnikovBoris MargulisIrina GuzhovaAlexander KaganskyAndrey L. KonevegaTatiana ShtamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luiza Garaeva Roman Kamyshinsky Yury Kil Elena Varfolomeeva Nikolai Verlov Elena Komarova Yuri Garmay Sergey Landa Vladimir Burdakov Alexander Myasnikov Ilya A. Vinnikov Boris Margulis Irina Guzhova Alexander Kagansky Andrey L. Konevega Tatiana Shtam Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
description	Abstract Plant-derived extracellular vesicles (EVs) gain more and more attention as promising carriers of exogenous bioactive molecules to the human cells. Derived from various edible sources, these EVs are remarkably biocompatible, biodegradable and highly abundant from plants. In this work, EVs from grapefruit juice were isolated by differential centrifugation followed by characterization of their size, quantity and morphology by nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy and cryo-electron microscopy (Cryo-EM). In Cryo-EM experiments, we visualized grapefruit EVs with the average size of 41 ± 13 nm, confirmed their round-shaped morphology and estimated the thickness of their lipid bilayer as 5.3 ± 0.8 nm. Further, using cell culture models, we have successfully demonstrated that native grapefruit-derived extracellular vesicles (GF-EVs) are highly efficient carriers for the delivery of the exogenous Alexa Fluor 647 labeled bovine serum albumin (BSA) and heat shock protein 70 (HSP70) into both human peripheral blood mononuclear cells and colon cancer cells. Interestingly, loading to plant EVs significantly ameliorated the uptake of exogenous proteins by human cells compared to the same proteins without EVs. Most importantly, we have confirmed the functional activity of human recombinant HSP70 in the colon cancer cell culture upon delivery by GF-EVs. Analysis of the biodistribution of GF-EVs loaded with 125I-labeled BSA in mice demonstrated a significant uptake of the grapefruit-derived extracellular vesicles by the majority of organs. The results of our study indicate that native plant EVs might be safe and effective carriers of exogenous proteins into human cells.
format	article
author	Luiza Garaeva Roman Kamyshinsky Yury Kil Elena Varfolomeeva Nikolai Verlov Elena Komarova Yuri Garmay Sergey Landa Vladimir Burdakov Alexander Myasnikov Ilya A. Vinnikov Boris Margulis Irina Guzhova Alexander Kagansky Andrey L. Konevega Tatiana Shtam
author_facet	Luiza Garaeva Roman Kamyshinsky Yury Kil Elena Varfolomeeva Nikolai Verlov Elena Komarova Yuri Garmay Sergey Landa Vladimir Burdakov Alexander Myasnikov Ilya A. Vinnikov Boris Margulis Irina Guzhova Alexander Kagansky Andrey L. Konevega Tatiana Shtam
author_sort	Luiza Garaeva
title	Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
title_short	Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
title_full	Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
title_fullStr	Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
title_full_unstemmed	Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
title_sort	delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/73fa49eb7021498780117d600e00ceee
work_keys_str_mv	AT luizagaraeva deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT romankamyshinsky deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT yurykil deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT elenavarfolomeeva deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT nikolaiverlov deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT elenakomarova deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT yurigarmay deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT sergeylanda deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT vladimirburdakov deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT alexandermyasnikov deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT ilyaavinnikov deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT borismargulis deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT irinaguzhova deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT alexanderkagansky deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT andreylkonevega deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro AT tatianashtam deliveryoffunctionalexogenousproteinsbyplantderivedvesiclestohumancellsinvitro
_version_	1718393101963231232

Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro

Ejemplares similares