Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

Wei Zhu,1,* Yi-Fang Meng,1,* Qian Xing,1 Jian-Jun Tao,1 Jiong Lu,1 Yan Wu2 1Department of Ophthalmology, Changshu No. 2 People’s Hospital, Changshu, China; 2Department of Ophthalmology, First Hospital Affiliated to Soochow University, Suzhou, China *These authors contributed equally to t...

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Autores principales:	Zhu W, Meng YF, Xing Q, Tao JJ, Lu J, Wu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	age-related macular degeneration lncRNAs microarray bioinformatics analyses. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/73fc275453554bfeb41547a1130755ca
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spelling	oai:doaj.org-article:73fc275453554bfeb41547a1130755ca2021-12-02T05:14:28ZIdentification of lncRNAs involved in biological regulation in early age-related macular degeneration1178-2013https://doaj.org/article/73fc275453554bfeb41547a1130755ca2017-10-01T00:00:00Zhttps://www.dovepress.com/identification-of-lncrnas-involved-in-biological-regulation-in-early-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Zhu,1,* Yi-Fang Meng,1,* Qian Xing,1 Jian-Jun Tao,1 Jiong Lu,1 Yan Wu2 1Department of Ophthalmology, Changshu No. 2 People’s Hospital, Changshu, China; 2Department of Ophthalmology, First Hospital Affiliated to Soochow University, Suzhou, China *These authors contributed equally to this work Background: Age-related macular degeneration (AMD) is one of the most common causes of adult blindness in developed countries. However, the role of long noncoding RNAs (lncRNAs) in the development and progression of early AMD is unclear.Methods: We established the lncRNA profile of early AMD by reannotation of microarrays from the gene expression omnibus database. Quantitative real-time polymerase chain reaction was used to determine the expression of selected lncRNAs.Results: The expression profiles of 9 cases of AMD and 7 controls were studied. A total of 266 differentially expressed genes (DEGs) were detected (94 upregulated and 172 downregulated). Among all the DEGs, 64 were lncRNAs. Advanced bioinformatics analyses demonstrated that differentially expressed lncRNAs could play significant roles in visual perception, sensory perception of light stimulus, and cognition. The pathway analyses showed that the two most significantly influenced Kyoto Encyclopedia of Genes and Genomes pathways were those of phototransduction and purine metabolism. By the analyses of the key lncRNAs, it was found that RP11-234O6.2 was downregulated in the aging retinal pigment epithelium (RPE) cellular model. Exogenous RP11-234O6.2 treatment led to increased cell viability and improved apoptosis but it did not affect the cell migration ability of aging RPE cells.Conclusion: This study indicated that lncRNAs are differentially expressed in early AMD and may produce important regulative effects. An lncRNA, RP11-234O6.2, might be involved in the biological regulation of early AMD and have therapeutic potential. Keywords: age-related macular degeneration, lncRNAs, microarray, bioinformatics analysesZhu WMeng YFXing QTao JJLu JWu YDove Medical Pressarticleage-related macular degenerationlncRNAsmicroarraybioinformatics analyses.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7589-7602 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	age-related macular degeneration lncRNAs microarray bioinformatics analyses. Medicine (General) R5-920
spellingShingle	age-related macular degeneration lncRNAs microarray bioinformatics analyses. Medicine (General) R5-920 Zhu W Meng YF Xing Q Tao JJ Lu J Wu Y Identification of lncRNAs involved in biological regulation in early age-related macular degeneration
description	Wei Zhu,1,* Yi-Fang Meng,1,* Qian Xing,1 Jian-Jun Tao,1 Jiong Lu,1 Yan Wu2 1Department of Ophthalmology, Changshu No. 2 People’s Hospital, Changshu, China; 2Department of Ophthalmology, First Hospital Affiliated to Soochow University, Suzhou, China *These authors contributed equally to this work Background: Age-related macular degeneration (AMD) is one of the most common causes of adult blindness in developed countries. However, the role of long noncoding RNAs (lncRNAs) in the development and progression of early AMD is unclear.Methods: We established the lncRNA profile of early AMD by reannotation of microarrays from the gene expression omnibus database. Quantitative real-time polymerase chain reaction was used to determine the expression of selected lncRNAs.Results: The expression profiles of 9 cases of AMD and 7 controls were studied. A total of 266 differentially expressed genes (DEGs) were detected (94 upregulated and 172 downregulated). Among all the DEGs, 64 were lncRNAs. Advanced bioinformatics analyses demonstrated that differentially expressed lncRNAs could play significant roles in visual perception, sensory perception of light stimulus, and cognition. The pathway analyses showed that the two most significantly influenced Kyoto Encyclopedia of Genes and Genomes pathways were those of phototransduction and purine metabolism. By the analyses of the key lncRNAs, it was found that RP11-234O6.2 was downregulated in the aging retinal pigment epithelium (RPE) cellular model. Exogenous RP11-234O6.2 treatment led to increased cell viability and improved apoptosis but it did not affect the cell migration ability of aging RPE cells.Conclusion: This study indicated that lncRNAs are differentially expressed in early AMD and may produce important regulative effects. An lncRNA, RP11-234O6.2, might be involved in the biological regulation of early AMD and have therapeutic potential. Keywords: age-related macular degeneration, lncRNAs, microarray, bioinformatics analyses
format	article
author	Zhu W Meng YF Xing Q Tao JJ Lu J Wu Y
author_facet	Zhu W Meng YF Xing Q Tao JJ Lu J Wu Y
author_sort	Zhu W
title	Identification of lncRNAs involved in biological regulation in early age-related macular degeneration
title_short	Identification of lncRNAs involved in biological regulation in early age-related macular degeneration
title_full	Identification of lncRNAs involved in biological regulation in early age-related macular degeneration
title_fullStr	Identification of lncRNAs involved in biological regulation in early age-related macular degeneration
title_full_unstemmed	Identification of lncRNAs involved in biological regulation in early age-related macular degeneration
title_sort	identification of lncrnas involved in biological regulation in early age-related macular degeneration
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/73fc275453554bfeb41547a1130755ca
work_keys_str_mv	AT zhuw identificationoflncrnasinvolvedinbiologicalregulationinearlyagerelatedmaculardegeneration AT mengyf identificationoflncrnasinvolvedinbiologicalregulationinearlyagerelatedmaculardegeneration AT xingq identificationoflncrnasinvolvedinbiologicalregulationinearlyagerelatedmaculardegeneration AT taojj identificationoflncrnasinvolvedinbiologicalregulationinearlyagerelatedmaculardegeneration AT luj identificationoflncrnasinvolvedinbiologicalregulationinearlyagerelatedmaculardegeneration AT wuy identificationoflncrnasinvolvedinbiologicalregulationinearlyagerelatedmaculardegeneration
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Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

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