<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients

The lipid tumour demand may shape the host metabolism adapting the circulating lipids composition to its growth and progression needs. This study aims to exploit the straightforward <sup>1</sup>H-NMR lipoproteins analysis to investigate the alterations of the circulating lipoproteins’ fr...

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Autores principales:	Giuseppe Corona, Emanuela Di Gregorio, Alessia Vignoli, Elena Muraro, Agostino Steffan, Gianmaria Miolo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<sup>1</sup>H-NMR breast cancer HER2- lipoproteins biomarkers diagnosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/7402cefcd5ff43749704f2b85652ef70
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spelling	oai:doaj.org-article:7402cefcd5ff43749704f2b85652ef702021-11-25T17:04:40Z<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients10.3390/cancers132258452072-6694https://doaj.org/article/7402cefcd5ff43749704f2b85652ef702021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5845https://doaj.org/toc/2072-6694The lipid tumour demand may shape the host metabolism adapting the circulating lipids composition to its growth and progression needs. This study aims to exploit the straightforward <sup>1</sup>H-NMR lipoproteins analysis to investigate the alterations of the circulating lipoproteins’ fractions in HER2-positive breast cancer and their modulations induced by treatments. The baseline <sup>1</sup>H-NMR plasma lipoproteins profiles were measured in 43 HER2-positive breast cancer patients and compared with those of 28 healthy women. In a subset of 32 patients, longitudinal measurements were also performed along neoadjuvant chemotherapy, after surgery, adjuvant treatment, and during the two-year follow-up. Differences between groups were assessed by multivariate PLS-DA and by univariate analyses. The diagnostic power of lipoproteins subfractions was assessed by ROC curve, while lipoproteins time changes along interventions were investigated by ANOVA analysis. The PLS-DA model distinguished HER2-positive breast cancer patients from the control group with a sensitivity of 96.4% and specificity of 90.7%, mainly due to the differential levels of VLDLs subfractions that were significantly higher in the patients’ group. Neoadjuvant chemotherapy-induced a significant drop in the HDLs after the first three months of treatment and a specific decrease in the HDL-3 and HDL-4 subfractions were found significantly associated with the pathological complete response achievement. These results indicate that HER2-positive breast cancer is characterized by a significant host lipid mobilization that could be useful for diagnostic purposes. Moreover, the lipoproteins profiles alterations induced by the therapeutic interventions could predict the clinical outcome supporting the application of <sup>1</sup>H-NMR lipoproteins profiles analysis for longitudinal monitoring of HER2-positive breast cancer in large clinical studies.Giuseppe CoronaEmanuela Di GregorioAlessia VignoliElena MuraroAgostino SteffanGianmaria MioloMDPI AGarticle<sup>1</sup>H-NMRbreast cancerHER2-lipoproteinsbiomarkersdiagnosisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5845, p 5845 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<sup>1</sup>H-NMR breast cancer HER2- lipoproteins biomarkers diagnosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	<sup>1</sup>H-NMR breast cancer HER2- lipoproteins biomarkers diagnosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Giuseppe Corona Emanuela Di Gregorio Alessia Vignoli Elena Muraro Agostino Steffan Gianmaria Miolo <sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients
description	The lipid tumour demand may shape the host metabolism adapting the circulating lipids composition to its growth and progression needs. This study aims to exploit the straightforward <sup>1</sup>H-NMR lipoproteins analysis to investigate the alterations of the circulating lipoproteins’ fractions in HER2-positive breast cancer and their modulations induced by treatments. The baseline <sup>1</sup>H-NMR plasma lipoproteins profiles were measured in 43 HER2-positive breast cancer patients and compared with those of 28 healthy women. In a subset of 32 patients, longitudinal measurements were also performed along neoadjuvant chemotherapy, after surgery, adjuvant treatment, and during the two-year follow-up. Differences between groups were assessed by multivariate PLS-DA and by univariate analyses. The diagnostic power of lipoproteins subfractions was assessed by ROC curve, while lipoproteins time changes along interventions were investigated by ANOVA analysis. The PLS-DA model distinguished HER2-positive breast cancer patients from the control group with a sensitivity of 96.4% and specificity of 90.7%, mainly due to the differential levels of VLDLs subfractions that were significantly higher in the patients’ group. Neoadjuvant chemotherapy-induced a significant drop in the HDLs after the first three months of treatment and a specific decrease in the HDL-3 and HDL-4 subfractions were found significantly associated with the pathological complete response achievement. These results indicate that HER2-positive breast cancer is characterized by a significant host lipid mobilization that could be useful for diagnostic purposes. Moreover, the lipoproteins profiles alterations induced by the therapeutic interventions could predict the clinical outcome supporting the application of <sup>1</sup>H-NMR lipoproteins profiles analysis for longitudinal monitoring of HER2-positive breast cancer in large clinical studies.
format	article
author	Giuseppe Corona Emanuela Di Gregorio Alessia Vignoli Elena Muraro Agostino Steffan Gianmaria Miolo
author_facet	Giuseppe Corona Emanuela Di Gregorio Alessia Vignoli Elena Muraro Agostino Steffan Gianmaria Miolo
author_sort	Giuseppe Corona
title	<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients
title_short	<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients
title_full	<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients
title_fullStr	<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients
title_full_unstemmed	<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients
title_sort	<sup>1</sup>h-nmr plasma lipoproteins profile analysis reveals lipid metabolism alterations in her2-positive breast cancer patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7402cefcd5ff43749704f2b85652ef70
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<sup>1</sup>H-NMR Plasma Lipoproteins Profile Analysis Reveals Lipid Metabolism Alterations in HER2-Positive Breast Cancer Patients

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