Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.

The plus-end microtubule binding proteins (+TIPs) play an important role in the regulation of microtubule stability and cell polarity during interphase. In S. pombe, the CLIP-170 like protein Tip1, together with the kinesin Tea2, moves along the microtubules towards their plus ends. Tip1 also requir...

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Autores principales:	Sherilyn Goldstone, Céline Reyes, Guillaume Gay, Thibault Courthéoux, Marion Dubarry, Sylvie Tournier, Yannick Gachet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7403b18f18ba48e6a898d0e1ce03ae72
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id	oai:doaj.org-article:7403b18f18ba48e6a898d0e1ce03ae72
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spelling	oai:doaj.org-article:7403b18f18ba48e6a898d0e1ce03ae722021-12-02T20:21:41ZTip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.1932-620310.1371/journal.pone.0010634https://doaj.org/article/7403b18f18ba48e6a898d0e1ce03ae722010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20498706/?tool=EBIhttps://doaj.org/toc/1932-6203The plus-end microtubule binding proteins (+TIPs) play an important role in the regulation of microtubule stability and cell polarity during interphase. In S. pombe, the CLIP-170 like protein Tip1, together with the kinesin Tea2, moves along the microtubules towards their plus ends. Tip1 also requires the EB1 homolog Mal3 to localize to the microtubule tips. Given the requirement for Tip1 for microtubule stability, we have investigated its role during spindle morphogenesis and chromosome movement. Loss of Tip1 affects metaphase plate formation and leads to the activation of the spindle assembly checkpoint. In the absence of Tip1 we also observed the appearance of lagging chromosomes, which do not influence the normal rate of spindle elongation. Our results suggest that S. pombe Tip1/CLIP170 is directly or indirectly required for correct chromosome poleward movement independently of Mal3/EB1.Sherilyn GoldstoneCéline ReyesGuillaume GayThibault CourthéouxMarion DubarrySylvie TournierYannick GachetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10634 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sherilyn Goldstone Céline Reyes Guillaume Gay Thibault Courthéoux Marion Dubarry Sylvie Tournier Yannick Gachet Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
description	The plus-end microtubule binding proteins (+TIPs) play an important role in the regulation of microtubule stability and cell polarity during interphase. In S. pombe, the CLIP-170 like protein Tip1, together with the kinesin Tea2, moves along the microtubules towards their plus ends. Tip1 also requires the EB1 homolog Mal3 to localize to the microtubule tips. Given the requirement for Tip1 for microtubule stability, we have investigated its role during spindle morphogenesis and chromosome movement. Loss of Tip1 affects metaphase plate formation and leads to the activation of the spindle assembly checkpoint. In the absence of Tip1 we also observed the appearance of lagging chromosomes, which do not influence the normal rate of spindle elongation. Our results suggest that S. pombe Tip1/CLIP170 is directly or indirectly required for correct chromosome poleward movement independently of Mal3/EB1.
format	article
author	Sherilyn Goldstone Céline Reyes Guillaume Gay Thibault Courthéoux Marion Dubarry Sylvie Tournier Yannick Gachet
author_facet	Sherilyn Goldstone Céline Reyes Guillaume Gay Thibault Courthéoux Marion Dubarry Sylvie Tournier Yannick Gachet
author_sort	Sherilyn Goldstone
title	Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
title_short	Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
title_full	Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
title_fullStr	Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
title_full_unstemmed	Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
title_sort	tip1/clip-170 protein is required for correct chromosome poleward movement in fission yeast.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/7403b18f18ba48e6a898d0e1ce03ae72
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Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.

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