New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo

Tianyang Zhou1,2, Xin Jia1, Huixiang Li3, Jin Wang3, Hongling Zhang1,2, Youmei A1,2, Zhenzhong Zhang1,21School of Pharmaceutical Sciences, 2Nanotechnology Research Center for Drugs, 3Department of Pathology, Medical School of Zhengzhou University, Zhengzhou, People&rsquo;s Republic of ChinaB...

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Autores principales:	Zhou T, Jia X, Li H, Wang J, Zhang H, A Y, Zhang ZZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/74073b80e17a412bb5bc026c2bbcbc20
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spelling	oai:doaj.org-article:74073b80e17a412bb5bc026c2bbcbc202021-12-02T05:55:48ZNew tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo1176-91141178-2013https://doaj.org/article/74073b80e17a412bb5bc026c2bbcbc202011-07-01T00:00:00Zhttp://www.dovepress.com/new-tumor-targeted-nanosized-delivery-carrier-for-oligonucleotides-cha-a7932https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Tianyang Zhou1,2, Xin Jia1, Huixiang Li3, Jin Wang3, Hongling Zhang1,2, Youmei A1,2, Zhenzhong Zhang1,21School of Pharmaceutical Sciences, 2Nanotechnology Research Center for Drugs, 3Department of Pathology, Medical School of Zhengzhou University, Zhengzhou, People&rsquo;s Republic of ChinaBackground: The purpose of this study was to investigate the in vitro and in vivo characteristics of a new tumor-targeted nanosized delivery carrier for antisense oligonucleotide (ASON).Methods: Polyethylenimine (PEI) was used to condense ASON to form nanosized complexes (PEI/ASON), which were then modified using asparagine-glycine-arginine (NGR) peptide to obtain a tumor-targeted nanosized delivery carrier (NGR/PEI/ASON). The conditions required to form PEI/ASON were investigated.Results: A linear correlation between the natural logarithm of the N/P ratio (PEI to ASON) and the zeta potential of the PEI/ASON complexes was found, ranging from 1.5 to 5.0. The pH of the solution strongly influenced the zeta potential of the PEI/ASON complexes. PEI/ASON and NGR/PEI/ASON were stable in RPMI-1640 culture medium in the presence of Dextran 70. Incorporation of ASON into PEI/ASON and NGR/PEI/ASON complexes prevented degradation of ASON by DNase I.Conclusion: Both ASON/PEI and NGR/PEI/ASON complexes enhanced the uptake of ASON by EC9706 cells in vitro. In vivo, NGR/PEI/ASON complexes had the ability to target tumor tissues effectively.Keywords: nanosized delivery system, squamous cell carcinoma, antisense oligonucleotideZhou TJia XLi HWang JZhang HA YZhang ZZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1527-1534 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhou T Jia X Li H Wang J Zhang H A Y Zhang ZZ New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
description	Tianyang Zhou1,2, Xin Jia1, Huixiang Li3, Jin Wang3, Hongling Zhang1,2, Youmei A1,2, Zhenzhong Zhang1,21School of Pharmaceutical Sciences, 2Nanotechnology Research Center for Drugs, 3Department of Pathology, Medical School of Zhengzhou University, Zhengzhou, People&rsquo;s Republic of ChinaBackground: The purpose of this study was to investigate the in vitro and in vivo characteristics of a new tumor-targeted nanosized delivery carrier for antisense oligonucleotide (ASON).Methods: Polyethylenimine (PEI) was used to condense ASON to form nanosized complexes (PEI/ASON), which were then modified using asparagine-glycine-arginine (NGR) peptide to obtain a tumor-targeted nanosized delivery carrier (NGR/PEI/ASON). The conditions required to form PEI/ASON were investigated.Results: A linear correlation between the natural logarithm of the N/P ratio (PEI to ASON) and the zeta potential of the PEI/ASON complexes was found, ranging from 1.5 to 5.0. The pH of the solution strongly influenced the zeta potential of the PEI/ASON complexes. PEI/ASON and NGR/PEI/ASON were stable in RPMI-1640 culture medium in the presence of Dextran 70. Incorporation of ASON into PEI/ASON and NGR/PEI/ASON complexes prevented degradation of ASON by DNase I.Conclusion: Both ASON/PEI and NGR/PEI/ASON complexes enhanced the uptake of ASON by EC9706 cells in vitro. In vivo, NGR/PEI/ASON complexes had the ability to target tumor tissues effectively.Keywords: nanosized delivery system, squamous cell carcinoma, antisense oligonucleotide
format	article
author	Zhou T Jia X Li H Wang J Zhang H A Y Zhang ZZ
author_facet	Zhou T Jia X Li H Wang J Zhang H A Y Zhang ZZ
author_sort	Zhou T
title	New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
title_short	New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
title_full	New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
title_fullStr	New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
title_full_unstemmed	New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
title_sort	new tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/74073b80e17a412bb5bc026c2bbcbc20
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New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo

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