Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13

Abstract Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrob...

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Autores principales:	Katarzyna Niemirowicz, Bonita Durnaś, Grażyna Tokajuk, Ewelina Piktel, Grzegorz Michalak, Xiaobo Gu, Alina Kułakowska, Paul B. Savage, Robert Bucki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7413342e159a47cb9f47c8beb2b5f9fb
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spelling	oai:doaj.org-article:7413342e159a47cb9f47c8beb2b5f9fb2021-12-02T15:05:08ZFormulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-1310.1038/s41598-017-04653-12045-2322https://doaj.org/article/7413342e159a47cb9f47c8beb2b5f9fb2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04653-1https://doaj.org/toc/2045-2322Abstract Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers. In this study, the fungicidal activities of LL-37 peptide, ceragenin CSA-13 and its magnetic derivatives (MNP@LL-37, MNP@CSA-13) against laboratory and clinical strains of C. albicans, C. glabrata and C. tropicalis were evaluated. These experiments confirm the high anti-fungal activity of these well-characterized agents mediated by their interaction with the fungal membrane and demonstrate elevated activity following immobilization of LL-37 and CSA-13 on the surface of magnetic nanoparticles (MNPs). Furthermore, MNP-based nanosystems are resistant to inhibitory factors present in body fluids and effectively inhibit formation of fungal biofilm. Simultaneously, synthesized nanostructures maintain immunomodulatory properties, described previously for free LL-37 peptide and CSA-13 substrate and they do not interfere with the proliferation and viability of osteoblasts, confirming their high biocompatibility.Katarzyna NiemirowiczBonita DurnaśGrażyna TokajukEwelina PiktelGrzegorz MichalakXiaobo GuAlina KułakowskaPaul B. SavageRobert BuckiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna Niemirowicz Bonita Durnaś Grażyna Tokajuk Ewelina Piktel Grzegorz Michalak Xiaobo Gu Alina Kułakowska Paul B. Savage Robert Bucki Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
description	Abstract Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers. In this study, the fungicidal activities of LL-37 peptide, ceragenin CSA-13 and its magnetic derivatives (MNP@LL-37, MNP@CSA-13) against laboratory and clinical strains of C. albicans, C. glabrata and C. tropicalis were evaluated. These experiments confirm the high anti-fungal activity of these well-characterized agents mediated by their interaction with the fungal membrane and demonstrate elevated activity following immobilization of LL-37 and CSA-13 on the surface of magnetic nanoparticles (MNPs). Furthermore, MNP-based nanosystems are resistant to inhibitory factors present in body fluids and effectively inhibit formation of fungal biofilm. Simultaneously, synthesized nanostructures maintain immunomodulatory properties, described previously for free LL-37 peptide and CSA-13 substrate and they do not interfere with the proliferation and viability of osteoblasts, confirming their high biocompatibility.
format	article
author	Katarzyna Niemirowicz Bonita Durnaś Grażyna Tokajuk Ewelina Piktel Grzegorz Michalak Xiaobo Gu Alina Kułakowska Paul B. Savage Robert Bucki
author_facet	Katarzyna Niemirowicz Bonita Durnaś Grażyna Tokajuk Ewelina Piktel Grzegorz Michalak Xiaobo Gu Alina Kułakowska Paul B. Savage Robert Bucki
author_sort	Katarzyna Niemirowicz
title	Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
title_short	Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
title_full	Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
title_fullStr	Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
title_full_unstemmed	Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
title_sort	formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin ll-37 and ceragenin csa-13
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7413342e159a47cb9f47c8beb2b5f9fb
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Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13

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