Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.

<h4>Background</h4>In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have...

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Autores principales:	Martin Christner, Maria Trusch, Holger Rohde, Marcel Kwiatkowski, Hartmut Schlüter, Manuel Wolters, Martin Aepfelbacher, Moritz Hentschke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/74157a1548d746fab3bd06e451d39486
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spelling	oai:doaj.org-article:74157a1548d746fab3bd06e451d394862021-11-25T06:09:20ZRapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.1932-620310.1371/journal.pone.0101924https://doaj.org/article/74157a1548d746fab3bd06e451d394862014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25003758/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have therefore evaluated the applicability of a MALDI-TOF mass spectrometry based strategy for outbreak strain identification.<h4>Methods</h4>Specific peaks in the outbreak strain's spectrum were identified by comparative analysis of archived pre-outbreak spectra that had been acquired for routine species-level identification. Proteins underlying these discriminatory peaks were identified by liquid chromatography tandem mass spectrometry and validated against publicly available databases. The resulting typing scheme was evaluated against PCR genotyping with 294 E. coli isolates from clinical samples collected during the outbreak.<h4>Results</h4>Comparative spectrum analysis revealed two characteristic peaks at m/z 6711 and m/z 10883. The underlying proteins were found to be of low prevalence among genome sequenced E. coli strains. Marker peak detection correctly classified 292 of 293 study isolates, including all 104 outbreak isolates.<h4>Conclusions</h4>MALDI-TOF mass spectrometry allowed for reliable outbreak strain identification during a large outbreak of Shiga-Toxigenic E. coli. The applied typing strategy could probably be adapted to other typing tasks and might facilitate epidemiological surveys as part of the routine pathogen identification workflow.Martin ChristnerMaria TruschHolger RohdeMarcel KwiatkowskiHartmut SchlüterManuel WoltersMartin AepfelbacherMoritz HentschkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101924 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Christner Maria Trusch Holger Rohde Marcel Kwiatkowski Hartmut Schlüter Manuel Wolters Martin Aepfelbacher Moritz Hentschke Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
description	<h4>Background</h4>In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have therefore evaluated the applicability of a MALDI-TOF mass spectrometry based strategy for outbreak strain identification.<h4>Methods</h4>Specific peaks in the outbreak strain's spectrum were identified by comparative analysis of archived pre-outbreak spectra that had been acquired for routine species-level identification. Proteins underlying these discriminatory peaks were identified by liquid chromatography tandem mass spectrometry and validated against publicly available databases. The resulting typing scheme was evaluated against PCR genotyping with 294 E. coli isolates from clinical samples collected during the outbreak.<h4>Results</h4>Comparative spectrum analysis revealed two characteristic peaks at m/z 6711 and m/z 10883. The underlying proteins were found to be of low prevalence among genome sequenced E. coli strains. Marker peak detection correctly classified 292 of 293 study isolates, including all 104 outbreak isolates.<h4>Conclusions</h4>MALDI-TOF mass spectrometry allowed for reliable outbreak strain identification during a large outbreak of Shiga-Toxigenic E. coli. The applied typing strategy could probably be adapted to other typing tasks and might facilitate epidemiological surveys as part of the routine pathogen identification workflow.
format	article
author	Martin Christner Maria Trusch Holger Rohde Marcel Kwiatkowski Hartmut Schlüter Manuel Wolters Martin Aepfelbacher Moritz Hentschke
author_facet	Martin Christner Maria Trusch Holger Rohde Marcel Kwiatkowski Hartmut Schlüter Manuel Wolters Martin Aepfelbacher Moritz Hentschke
author_sort	Martin Christner
title	Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_short	Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_full	Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_fullStr	Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_full_unstemmed	Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_sort	rapid maldi-tof mass spectrometry strain typing during a large outbreak of shiga-toxigenic escherichia coli.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/74157a1548d746fab3bd06e451d39486
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Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.

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