Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage

Current surgical techniques to treat articular cartilage defects fail to produce a satisfactory long-term repair of the tissue. Regenerative approaches show promise in their ability to generate hyaline cartilage using biomaterials in combination with stem cells. However, the difficulty of seamlessly...

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Autores principales:	Anna Trengove, Serena Duchi, Carmine Onofrillo, Cathal D. O'Connell, Claudia Di Bella, Andrea J. O'Connor
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioadhesive cartilage repair transglutaminase GelMA integration tissue engineering Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/741ad1c1ef2f427ba444c854a0fb505e
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spelling	oai:doaj.org-article:741ad1c1ef2f427ba444c854a0fb505e2021-11-18T07:39:28ZMicrobial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage2673-312910.3389/fmedt.2021.773673https://doaj.org/article/741ad1c1ef2f427ba444c854a0fb505e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmedt.2021.773673/fullhttps://doaj.org/toc/2673-3129Current surgical techniques to treat articular cartilage defects fail to produce a satisfactory long-term repair of the tissue. Regenerative approaches show promise in their ability to generate hyaline cartilage using biomaterials in combination with stem cells. However, the difficulty of seamlessly integrating the newly generated cartilage with the surrounding tissue remains a likely cause of long-term failure. To begin to address this integration issue, our strategy exploits a biological enzyme (microbial transglutaminase) to effect bioadhesion of a gelatin methacryloyl implant to host tissue. Mechanical characterization of the bioadhesive material shows that enzymatic crosslinking is compatible with photocrosslinking, allowing for a dual-crosslinked system with improved mechanical properties, and a slower degradation rate. Biocompatibility is illustrated with a 3D study of the metabolic activity of encapsulated human adipose derived stem cells. Furthermore, enzymatic crosslinking induced by transglutaminase is not prevented by the presence of cells, as measured by the bulk modulus of the material. Adhesion to human cartilage is demonstrated ex vivo with a significant increase in adhesive strength (5.82 ± 1.4 kPa as compared to 2.87 ± 0.9 kPa, p < 0.01) due to the addition of transglutaminase. For the first time, we have characterized a bioadhesive material composed of microbial transglutaminase and GelMA that can encapsulate cells, be photo crosslinked, and bond to host cartilage, taking a step toward the integration of regenerative implants.Anna TrengoveAnna TrengoveSerena DuchiSerena DuchiCarmine OnofrilloCarmine OnofrilloCathal D. O'ConnellCathal D. O'ConnellClaudia Di BellaClaudia Di BellaClaudia Di BellaAndrea J. O'ConnorAndrea J. O'ConnorFrontiers Media S.A.articlebioadhesivecartilage repairtransglutaminaseGelMAintegrationtissue engineeringMedical technologyR855-855.5ENFrontiers in Medical Technology, Vol 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioadhesive cartilage repair transglutaminase GelMA integration tissue engineering Medical technology R855-855.5
spellingShingle	bioadhesive cartilage repair transglutaminase GelMA integration tissue engineering Medical technology R855-855.5 Anna Trengove Anna Trengove Serena Duchi Serena Duchi Carmine Onofrillo Carmine Onofrillo Cathal D. O'Connell Cathal D. O'Connell Claudia Di Bella Claudia Di Bella Claudia Di Bella Andrea J. O'Connor Andrea J. O'Connor Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage
description	Current surgical techniques to treat articular cartilage defects fail to produce a satisfactory long-term repair of the tissue. Regenerative approaches show promise in their ability to generate hyaline cartilage using biomaterials in combination with stem cells. However, the difficulty of seamlessly integrating the newly generated cartilage with the surrounding tissue remains a likely cause of long-term failure. To begin to address this integration issue, our strategy exploits a biological enzyme (microbial transglutaminase) to effect bioadhesion of a gelatin methacryloyl implant to host tissue. Mechanical characterization of the bioadhesive material shows that enzymatic crosslinking is compatible with photocrosslinking, allowing for a dual-crosslinked system with improved mechanical properties, and a slower degradation rate. Biocompatibility is illustrated with a 3D study of the metabolic activity of encapsulated human adipose derived stem cells. Furthermore, enzymatic crosslinking induced by transglutaminase is not prevented by the presence of cells, as measured by the bulk modulus of the material. Adhesion to human cartilage is demonstrated ex vivo with a significant increase in adhesive strength (5.82 ± 1.4 kPa as compared to 2.87 ± 0.9 kPa, p < 0.01) due to the addition of transglutaminase. For the first time, we have characterized a bioadhesive material composed of microbial transglutaminase and GelMA that can encapsulate cells, be photo crosslinked, and bond to host cartilage, taking a step toward the integration of regenerative implants.
format	article
author	Anna Trengove Anna Trengove Serena Duchi Serena Duchi Carmine Onofrillo Carmine Onofrillo Cathal D. O'Connell Cathal D. O'Connell Claudia Di Bella Claudia Di Bella Claudia Di Bella Andrea J. O'Connor Andrea J. O'Connor
author_facet	Anna Trengove Anna Trengove Serena Duchi Serena Duchi Carmine Onofrillo Carmine Onofrillo Cathal D. O'Connell Cathal D. O'Connell Claudia Di Bella Claudia Di Bella Claudia Di Bella Andrea J. O'Connor Andrea J. O'Connor
author_sort	Anna Trengove
title	Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage
title_short	Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage
title_full	Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage
title_fullStr	Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage
title_full_unstemmed	Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage
title_sort	microbial transglutaminase improves ex vivo adhesion of gelatin methacryloyl hydrogels to human cartilage
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/741ad1c1ef2f427ba444c854a0fb505e
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Microbial Transglutaminase Improves ex vivo Adhesion of Gelatin Methacryloyl Hydrogels to Human Cartilage

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