Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.

Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F2∶3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and...

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Autores principales:	Jiqing Zhang, Changjian Xia, Canxing Duan, Suli Sun, Xiaoming Wang, Xiaofei Wu, Zhendong Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/741c32eb824448b89cd0a0982a46677d
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spelling	oai:doaj.org-article:741c32eb824448b89cd0a0982a46677d2021-11-18T09:02:54ZIdentification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.1932-620310.1371/journal.pone.0069799https://doaj.org/article/741c32eb824448b89cd0a0982a46677d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936102/?tool=EBIhttps://doaj.org/toc/1932-6203Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F2∶3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd15-24/25 and Sattwd15-47 flanked the resistance gene at a distance of 0.5 cM and 0.8 cM, respectively. Two cosegregating markers, Sattwd15-28 and Sattwd15-32, were also screened in this region. This is the first Rps resistance gene mapped on chromosome 17, which is designated as Rps10. Eight putative genes were found in the mapped region between markers Sattwd15-24/25 and Sattwd15-47. Among them, two candidate genes encoding serine/threonine (Ser/Thr) protein kinases in Wandou 15 and Williams were identified and sequenced. And the differences in genomic sequence and the putative amino acid sequence, respectively, were identified within each candidate gene between Wandou 15 and Williams. This novel gene Rps10 and the linked markers should be useful in developing soybean cultivars with durable resistance to P. sojae.Jiqing ZhangChangjian XiaCanxing DuanSuli SunXiaoming WangXiaofei WuZhendong ZhuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69799 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiqing Zhang Changjian Xia Canxing Duan Suli Sun Xiaoming Wang Xiaofei Wu Zhendong Zhu Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.
description	Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F2∶3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd15-24/25 and Sattwd15-47 flanked the resistance gene at a distance of 0.5 cM and 0.8 cM, respectively. Two cosegregating markers, Sattwd15-28 and Sattwd15-32, were also screened in this region. This is the first Rps resistance gene mapped on chromosome 17, which is designated as Rps10. Eight putative genes were found in the mapped region between markers Sattwd15-24/25 and Sattwd15-47. Among them, two candidate genes encoding serine/threonine (Ser/Thr) protein kinases in Wandou 15 and Williams were identified and sequenced. And the differences in genomic sequence and the putative amino acid sequence, respectively, were identified within each candidate gene between Wandou 15 and Williams. This novel gene Rps10 and the linked markers should be useful in developing soybean cultivars with durable resistance to P. sojae.
format	article
author	Jiqing Zhang Changjian Xia Canxing Duan Suli Sun Xiaoming Wang Xiaofei Wu Zhendong Zhu
author_facet	Jiqing Zhang Changjian Xia Canxing Duan Suli Sun Xiaoming Wang Xiaofei Wu Zhendong Zhu
author_sort	Jiqing Zhang
title	Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.
title_short	Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.
title_full	Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.
title_fullStr	Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.
title_full_unstemmed	Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.
title_sort	identification and candidate gene analysis of a novel phytophthora resistance gene rps10 in a chinese soybean cultivar.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/741c32eb824448b89cd0a0982a46677d
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Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar.

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