Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer

Abstract The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies agains...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Melike Kurt, Zeki Yumuk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/741fe68253894ca8b4f050f6999c8410
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:741fe68253894ca8b4f050f6999c8410
record_format	dspace
spelling	oai:doaj.org-article:741fe68253894ca8b4f050f6999c84102021-12-02T14:12:41ZDiagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer10.1038/s41598-021-81171-12045-2322https://doaj.org/article/741fe68253894ca8b4f050f6999c84102021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81171-1https://doaj.org/toc/2045-2322Abstract The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies against Fusobacterium nucleatum in the diagnosis of colorectal cancer (CRC). Totally 78 patients in three groups were included in the study. F. nucleatum in the tissues was detected using quantitative polymerase chain reaction assay. F. nucleatum IgA and IgG were measured using enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% cases of CRC and precancerous-benign colon disease (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA tests were higher in CRC group compared with healthy individuals. The sensitivity of IgA ELISA test varied between 31.8 and 95.5% depending on the chosen cut-off values. The positivity rate of antibodies in patients with high amount of F. nucleatum in tissue was significantly greater than in the negative group. The F. nucleatum IgA and IgG antibodies in CRC were higher than the ones in healthy controls but the discriminative ability of the ELISA test was not adequate to be considered as a diagnostic tool.Melike KurtZeki YumukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melike Kurt Zeki Yumuk Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
description	Abstract The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies against Fusobacterium nucleatum in the diagnosis of colorectal cancer (CRC). Totally 78 patients in three groups were included in the study. F. nucleatum in the tissues was detected using quantitative polymerase chain reaction assay. F. nucleatum IgA and IgG were measured using enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% cases of CRC and precancerous-benign colon disease (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA tests were higher in CRC group compared with healthy individuals. The sensitivity of IgA ELISA test varied between 31.8 and 95.5% depending on the chosen cut-off values. The positivity rate of antibodies in patients with high amount of F. nucleatum in tissue was significantly greater than in the negative group. The F. nucleatum IgA and IgG antibodies in CRC were higher than the ones in healthy controls but the discriminative ability of the ELISA test was not adequate to be considered as a diagnostic tool.
format	article
author	Melike Kurt Zeki Yumuk
author_facet	Melike Kurt Zeki Yumuk
author_sort	Melike Kurt
title	Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
title_short	Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
title_full	Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
title_fullStr	Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
title_full_unstemmed	Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
title_sort	diagnostic accuracy of fusobacterium nucleatum iga and igg elisa test in colorectal cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/741fe68253894ca8b4f050f6999c8410
work_keys_str_mv	AT melikekurt diagnosticaccuracyoffusobacteriumnucleatumigaandiggelisatestincolorectalcancer AT zekiyumuk diagnosticaccuracyoffusobacteriumnucleatumigaandiggelisatestincolorectalcancer
_version_	1718391790556413952

Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer

Ejemplares similares