Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland

Abstract Transporters involved in bile acid (BA) handling by the mammary gland are poorly understood. Here we have investigated the role of ABC proteins in blood-milk BA traffic and its sensitivity to maternal cholestasis. BA concentrations in rat and mouse serum were higher than in milk. BA profile...

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Autores principales:	Alba M. G. Blazquez, Rocio I. R. Macias, Candela Cives-Losada, Alberto de la Iglesia, Jose J. G. Marin, Maria J. Monte
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7420089d0fcb4fc2bbcafa376e74fce2
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spelling	oai:doaj.org-article:7420089d0fcb4fc2bbcafa376e74fce22021-12-02T15:05:49ZLactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland10.1038/s41598-017-06315-82045-2322https://doaj.org/article/7420089d0fcb4fc2bbcafa376e74fce22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06315-8https://doaj.org/toc/2045-2322Abstract Transporters involved in bile acid (BA) handling by the mammary gland are poorly understood. Here we have investigated the role of ABC proteins in blood-milk BA traffic and its sensitivity to maternal cholestasis. BA concentrations in rat and mouse serum were higher than in milk. BA profiles in both fluids were also different. In mammary gland, mRNA levels of ABC pumps transporting BAs were high for Bcrp, less abundant for Mrp1, Mrp3 and Mrp4 and negligible for Bsep and Mrp2. Milk BA concentrations were lower in Abcg2 −/− than in wild-type mice. Taurocholate administration (5 µmol, i.p.) increased 20-fold BA concentrations in serum, but only moderately in milk, even in Abcg2 −/− mice. Bile duct ligation (BDL) in pregnant rats markedly increased serum BA concentrations, which was not proportionally reflected in milk. In rat mammary tissue, Mrp4 was up-regulated by BDL. Serum BA levels were 2-fold higher in 10-day-old neonates of the BDL group, whereas their body weight was lower. The exchange of breastfeeding mothers immediately after birth reverted the situation without changes in endogenous BA synthesis. In conclusion, Bcrp is involved in BA secretion into milk, whereas Mrp4 participates in a blood-milk barrier that protects neonates from maternal hypercholanemia during breastfeeding.Alba M. G. BlazquezRocio I. R. MaciasCandela Cives-LosadaAlberto de la IglesiaJose J. G. MarinMaria J. MonteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alba M. G. Blazquez Rocio I. R. Macias Candela Cives-Losada Alberto de la Iglesia Jose J. G. Marin Maria J. Monte Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland
description	Abstract Transporters involved in bile acid (BA) handling by the mammary gland are poorly understood. Here we have investigated the role of ABC proteins in blood-milk BA traffic and its sensitivity to maternal cholestasis. BA concentrations in rat and mouse serum were higher than in milk. BA profiles in both fluids were also different. In mammary gland, mRNA levels of ABC pumps transporting BAs were high for Bcrp, less abundant for Mrp1, Mrp3 and Mrp4 and negligible for Bsep and Mrp2. Milk BA concentrations were lower in Abcg2 −/− than in wild-type mice. Taurocholate administration (5 µmol, i.p.) increased 20-fold BA concentrations in serum, but only moderately in milk, even in Abcg2 −/− mice. Bile duct ligation (BDL) in pregnant rats markedly increased serum BA concentrations, which was not proportionally reflected in milk. In rat mammary tissue, Mrp4 was up-regulated by BDL. Serum BA levels were 2-fold higher in 10-day-old neonates of the BDL group, whereas their body weight was lower. The exchange of breastfeeding mothers immediately after birth reverted the situation without changes in endogenous BA synthesis. In conclusion, Bcrp is involved in BA secretion into milk, whereas Mrp4 participates in a blood-milk barrier that protects neonates from maternal hypercholanemia during breastfeeding.
format	article
author	Alba M. G. Blazquez Rocio I. R. Macias Candela Cives-Losada Alberto de la Iglesia Jose J. G. Marin Maria J. Monte
author_facet	Alba M. G. Blazquez Rocio I. R. Macias Candela Cives-Losada Alberto de la Iglesia Jose J. G. Marin Maria J. Monte
author_sort	Alba M. G. Blazquez
title	Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland
title_short	Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland
title_full	Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland
title_fullStr	Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland
title_full_unstemmed	Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland
title_sort	lactation during cholestasis: role of abc proteins in bile acid traffic across the mammary gland
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7420089d0fcb4fc2bbcafa376e74fce2
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Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland

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