Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood

Abstract Chronic inflammation is one of the causes of neurodegeneration in Amyotrophic lateral sclerosis (ALS). Here we examined whether circulating dendritic cells (DCs) can contribute to disease progression. We found ALS patients show a significant reduction in the number of circulating DCs. Also,...

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Autores principales:	Michela Rusconi, Francesca Gerardi, William Santus, Andrea Lizio, Valeria Ada Sansone, Christian Lunetta, Ivan Zanoni, Francesca Granucci
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/74215155912e4d13868710927fcc8a11
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spelling	oai:doaj.org-article:74215155912e4d13868710927fcc8a112021-12-02T16:06:06ZInflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood10.1038/s41598-017-08233-12045-2322https://doaj.org/article/74215155912e4d13868710927fcc8a112017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08233-1https://doaj.org/toc/2045-2322Abstract Chronic inflammation is one of the causes of neurodegeneration in Amyotrophic lateral sclerosis (ALS). Here we examined whether circulating dendritic cells (DCs) can contribute to disease progression. We found ALS patients show a significant reduction in the number of circulating DCs. Also, patients’ DCs present an increased expression of CD62L and a tendency to overexpress CCR2 compared with healthy donors. Moreover, DCs derived from a subpopulation of ALS patients produced higher levels of IL-8 and CCL-2 upon lipopolysaccharide (LPS)-stimulation. Finally, we found a significant inverse correlation between the time from onset of the pathology to its diagnosis and the levels of IL-6 secretion induced by LPS. Our data support the hypothesis, in a subpopulation of patients, DCs recruited at the diseased tissue produce high levels of CCL-2 and IL-8 and contribute to the inflammatory process promoting the recruitment of other inflammatory cells. An increased efficiency of IL-6 production may accelerate only the initial phases of disease progression. Blood DC analysis can be used to identify ALS patients with an altered course of inflammatory cell recruitment at the diseased central nervous system (CNS). The high levels of CD62L expression suggests this molecule could be a target for treatment of CNS inflammation.Michela RusconiFrancesca GerardiWilliam SantusAndrea LizioValeria Ada SansoneChristian LunettaIvan ZanoniFrancesca GranucciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michela Rusconi Francesca Gerardi William Santus Andrea Lizio Valeria Ada Sansone Christian Lunetta Ivan Zanoni Francesca Granucci Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood
description	Abstract Chronic inflammation is one of the causes of neurodegeneration in Amyotrophic lateral sclerosis (ALS). Here we examined whether circulating dendritic cells (DCs) can contribute to disease progression. We found ALS patients show a significant reduction in the number of circulating DCs. Also, patients’ DCs present an increased expression of CD62L and a tendency to overexpress CCR2 compared with healthy donors. Moreover, DCs derived from a subpopulation of ALS patients produced higher levels of IL-8 and CCL-2 upon lipopolysaccharide (LPS)-stimulation. Finally, we found a significant inverse correlation between the time from onset of the pathology to its diagnosis and the levels of IL-6 secretion induced by LPS. Our data support the hypothesis, in a subpopulation of patients, DCs recruited at the diseased tissue produce high levels of CCL-2 and IL-8 and contribute to the inflammatory process promoting the recruitment of other inflammatory cells. An increased efficiency of IL-6 production may accelerate only the initial phases of disease progression. Blood DC analysis can be used to identify ALS patients with an altered course of inflammatory cell recruitment at the diseased central nervous system (CNS). The high levels of CD62L expression suggests this molecule could be a target for treatment of CNS inflammation.
format	article
author	Michela Rusconi Francesca Gerardi William Santus Andrea Lizio Valeria Ada Sansone Christian Lunetta Ivan Zanoni Francesca Granucci
author_facet	Michela Rusconi Francesca Gerardi William Santus Andrea Lizio Valeria Ada Sansone Christian Lunetta Ivan Zanoni Francesca Granucci
author_sort	Michela Rusconi
title	Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood
title_short	Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood
title_full	Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood
title_fullStr	Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood
title_full_unstemmed	Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood
title_sort	inflammatory role of dendritic cells in amyotrophic lateral sclerosis revealed by an analysis of patients’ peripheral blood
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/74215155912e4d13868710927fcc8a11
work_keys_str_mv	AT michelarusconi inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT francescagerardi inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT williamsantus inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT andrealizio inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT valeriaadasansone inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT christianlunetta inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT ivanzanoni inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood AT francescagranucci inflammatoryroleofdendriticcellsinamyotrophiclateralsclerosisrevealedbyananalysisofpatientsperipheralblood
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Inflammatory role of dendritic cells in Amyotrophic Lateral Sclerosis revealed by an analysis of patients’ peripheral blood

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