Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals

Abstract Biomimetic synthesis of nanoparticles offers a convenient and bio friendly approach to fabricate complex structures with sub-nanometer precision from simple precursor components. In the present study, we have synthesized nanoparticles of Amphotericin B (AmB), a potent antifungal agent, usin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qamar Zia, Owais Mohammad, Mohd Ahmar Rauf, Wasi Khan, Swaleha Zubair
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7424724caecb42349e34e2ad583212aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7424724caecb42349e34e2ad583212aa
record_format	dspace
spelling	oai:doaj.org-article:7424724caecb42349e34e2ad583212aa2021-12-02T11:41:00ZBiomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals10.1038/s41598-017-11847-02045-2322https://doaj.org/article/7424724caecb42349e34e2ad583212aa2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-11847-0https://doaj.org/toc/2045-2322Abstract Biomimetic synthesis of nanoparticles offers a convenient and bio friendly approach to fabricate complex structures with sub-nanometer precision from simple precursor components. In the present study, we have synthesized nanoparticles of Amphotericin B (AmB), a potent antifungal agent, using Aloe vera leaf extract. The synthesis of AmB nano-assemblies (AmB-NAs) was established employing spectro-photometric and electron microscopic studies, while their crystalline nature was established by X-ray diffraction. AmB-nano-formulation showed much higher stability in both phosphate buffer saline and serum and exhibit sustained release of parent drug over an extended time period. The as-synthesized AmB-NA possessed significantly less haemolysis as well as nephrotoxicity in the host at par with Ambisome®, a liposomized AmB formulation. Interestingly, the AmB-NAs were more effective in killing various fungal pathogens including Candida spp. and evoked less drug related toxic manifestations in the host as compared to free form of the drug. The data of the present study suggest that biomimetically synthesized AmB-NA circumvent toxicity issues and offer a promising approach to eliminate systemic fungal infections in Balb/C mice.Qamar ZiaOwais MohammadMohd Ahmar RaufWasi KhanSwaleha ZubairNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-19 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qamar Zia Owais Mohammad Mohd Ahmar Rauf Wasi Khan Swaleha Zubair Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
description	Abstract Biomimetic synthesis of nanoparticles offers a convenient and bio friendly approach to fabricate complex structures with sub-nanometer precision from simple precursor components. In the present study, we have synthesized nanoparticles of Amphotericin B (AmB), a potent antifungal agent, using Aloe vera leaf extract. The synthesis of AmB nano-assemblies (AmB-NAs) was established employing spectro-photometric and electron microscopic studies, while their crystalline nature was established by X-ray diffraction. AmB-nano-formulation showed much higher stability in both phosphate buffer saline and serum and exhibit sustained release of parent drug over an extended time period. The as-synthesized AmB-NA possessed significantly less haemolysis as well as nephrotoxicity in the host at par with Ambisome®, a liposomized AmB formulation. Interestingly, the AmB-NAs were more effective in killing various fungal pathogens including Candida spp. and evoked less drug related toxic manifestations in the host as compared to free form of the drug. The data of the present study suggest that biomimetically synthesized AmB-NA circumvent toxicity issues and offer a promising approach to eliminate systemic fungal infections in Balb/C mice.
format	article
author	Qamar Zia Owais Mohammad Mohd Ahmar Rauf Wasi Khan Swaleha Zubair
author_facet	Qamar Zia Owais Mohammad Mohd Ahmar Rauf Wasi Khan Swaleha Zubair
author_sort	Qamar Zia
title	Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
title_short	Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
title_full	Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
title_fullStr	Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
title_full_unstemmed	Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
title_sort	biomimetically engineered amphotericin b nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7424724caecb42349e34e2ad583212aa
work_keys_str_mv	AT qamarzia biomimeticallyengineeredamphotericinbnanoaggregatescircumventtoxicityconstraintsandtreatsystemicfungalinfectioninexperimentalanimals AT owaismohammad biomimeticallyengineeredamphotericinbnanoaggregatescircumventtoxicityconstraintsandtreatsystemicfungalinfectioninexperimentalanimals AT mohdahmarrauf biomimeticallyengineeredamphotericinbnanoaggregatescircumventtoxicityconstraintsandtreatsystemicfungalinfectioninexperimentalanimals AT wasikhan biomimeticallyengineeredamphotericinbnanoaggregatescircumventtoxicityconstraintsandtreatsystemicfungalinfectioninexperimentalanimals AT swalehazubair biomimeticallyengineeredamphotericinbnanoaggregatescircumventtoxicityconstraintsandtreatsystemicfungalinfectioninexperimentalanimals
_version_	1718395467280154624

Biomimetically engineered Amphotericin B nano-aggregates circumvent toxicity constraints and treat systemic fungal infection in experimental animals

Ejemplares similares