Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches

The current study was designed to isolate and characterize some bioactive secondary metabolites by using repeated chromatographic and spectroscopic techniques, targeting their anticancer, antimicrobial, and antioxidant properties through in vitro and in silico approaches. Six compounds were isolated...

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Autores principales:	Most. Chand Sultana Khatun, Md. Abdul Muhit, Md. Jamal Hossain, Muhammad Abdullah Al-Mansur, S.M. Abdur Rahman
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Stevia rebaudiana Phenolic constituents Cytotoxicity Antimicrobial Antioxidant Molecular docking Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/742be85c42e9427ca7a6c53db38839ea
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spelling	oai:doaj.org-article:742be85c42e9427ca7a6c53db38839ea2021-12-02T05:03:22ZIsolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches2405-844010.1016/j.heliyon.2021.e08475https://doaj.org/article/742be85c42e9427ca7a6c53db38839ea2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021025780https://doaj.org/toc/2405-8440The current study was designed to isolate and characterize some bioactive secondary metabolites by using repeated chromatographic and spectroscopic techniques, targeting their anticancer, antimicrobial, and antioxidant properties through in vitro and in silico approaches. Six compounds were isolated and analyzed by thin layer chromatographic technique and the compounds were identified as 5-O-caffeoyl quinic acid (1), syringin (2), luteolin (3), apigenin (4), jhanol (5), and jhanidiol (6) based on spectroscopic methods. The cytotoxic effect of each compound was dose-dependent, and compound 1 showed a higher anti-proliferative effect (IC50 = 181.3 μg/ml) than other compounds (compound 2, 4, 5, and 6). Besides, compound 1 showed the most promising antibacterial activity with a zone of inhibition ranges from 12–15 mm against different strains compared to ciprofloxacin (14–22 mm). In contrast, compound 3 exerted the highest scavenging property against DPPH free radical. Finally, the in vitro bioactivities were also supported by molecular docking studies. The computational study demonstrated that the isolated compounds exerted stronger affinity compared to the standard drugs towards the binding sites of dihydrofolate reductase (DHFR), glutathione reductase, and urase oxidase.Most. Chand Sultana KhatunMd. Abdul MuhitMd. Jamal HossainMuhammad Abdullah Al-MansurS.M. Abdur RahmanElsevierarticleStevia rebaudianaPhenolic constituentsCytotoxicityAntimicrobialAntioxidantMolecular dockingScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 12, Pp e08475- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Stevia rebaudiana Phenolic constituents Cytotoxicity Antimicrobial Antioxidant Molecular docking Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Stevia rebaudiana Phenolic constituents Cytotoxicity Antimicrobial Antioxidant Molecular docking Science (General) Q1-390 Social sciences (General) H1-99 Most. Chand Sultana Khatun Md. Abdul Muhit Md. Jamal Hossain Muhammad Abdullah Al-Mansur S.M. Abdur Rahman Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
description	The current study was designed to isolate and characterize some bioactive secondary metabolites by using repeated chromatographic and spectroscopic techniques, targeting their anticancer, antimicrobial, and antioxidant properties through in vitro and in silico approaches. Six compounds were isolated and analyzed by thin layer chromatographic technique and the compounds were identified as 5-O-caffeoyl quinic acid (1), syringin (2), luteolin (3), apigenin (4), jhanol (5), and jhanidiol (6) based on spectroscopic methods. The cytotoxic effect of each compound was dose-dependent, and compound 1 showed a higher anti-proliferative effect (IC50 = 181.3 μg/ml) than other compounds (compound 2, 4, 5, and 6). Besides, compound 1 showed the most promising antibacterial activity with a zone of inhibition ranges from 12–15 mm against different strains compared to ciprofloxacin (14–22 mm). In contrast, compound 3 exerted the highest scavenging property against DPPH free radical. Finally, the in vitro bioactivities were also supported by molecular docking studies. The computational study demonstrated that the isolated compounds exerted stronger affinity compared to the standard drugs towards the binding sites of dihydrofolate reductase (DHFR), glutathione reductase, and urase oxidase.
format	article
author	Most. Chand Sultana Khatun Md. Abdul Muhit Md. Jamal Hossain Muhammad Abdullah Al-Mansur S.M. Abdur Rahman
author_facet	Most. Chand Sultana Khatun Md. Abdul Muhit Md. Jamal Hossain Muhammad Abdullah Al-Mansur S.M. Abdur Rahman
author_sort	Most. Chand Sultana Khatun
title	Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
title_short	Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
title_full	Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
title_fullStr	Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
title_full_unstemmed	Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
title_sort	isolation of phytochemical constituents from stevia rebaudiana (bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/742be85c42e9427ca7a6c53db38839ea
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Isolation of phytochemical constituents from Stevia rebaudiana (Bert.) and evaluation of their anticancer, antimicrobial and antioxidant properties via in vitro and in silico approaches

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