Engineering subtilisin proteases that specifically degrade active RAS

Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro...

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Autores principales:	Yingwei Chen, Eric A. Toth, Biao Ruan, Eun Jung Choi, Richard Simmerman, Yihong Chen, Yanan He, Ruixue Wang, Raquel Godoy-Ruiz, Harlan King, Gregory Custer, D. Travis Gallagher, David A. Rozak, Melani Solomon, Silvia Muro, David J. Weber, John Orban, Thomas R. Fuerst, Philip N. Bryan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/742c1a3e0584400b8b631c6e4a1b0ff6
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spelling	oai:doaj.org-article:742c1a3e0584400b8b631c6e4a1b0ff62021-12-02T13:19:28ZEngineering subtilisin proteases that specifically degrade active RAS10.1038/s42003-021-01818-72399-3642https://doaj.org/article/742c1a3e0584400b8b631c6e4a1b0ff62021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01818-7https://doaj.org/toc/2399-3642Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro and in cells with a promise of adaptability for other target proteins too.Yingwei ChenEric A. TothBiao RuanEun Jung ChoiRichard SimmermanYihong ChenYanan HeRuixue WangRaquel Godoy-RuizHarlan KingGregory CusterD. Travis GallagherDavid A. RozakMelani SolomonSilvia MuroDavid J. WeberJohn OrbanThomas R. FuerstPhilip N. BryanNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yingwei Chen Eric A. Toth Biao Ruan Eun Jung Choi Richard Simmerman Yihong Chen Yanan He Ruixue Wang Raquel Godoy-Ruiz Harlan King Gregory Custer D. Travis Gallagher David A. Rozak Melani Solomon Silvia Muro David J. Weber John Orban Thomas R. Fuerst Philip N. Bryan Engineering subtilisin proteases that specifically degrade active RAS
description	Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro and in cells with a promise of adaptability for other target proteins too.
format	article
author	Yingwei Chen Eric A. Toth Biao Ruan Eun Jung Choi Richard Simmerman Yihong Chen Yanan He Ruixue Wang Raquel Godoy-Ruiz Harlan King Gregory Custer D. Travis Gallagher David A. Rozak Melani Solomon Silvia Muro David J. Weber John Orban Thomas R. Fuerst Philip N. Bryan
author_facet	Yingwei Chen Eric A. Toth Biao Ruan Eun Jung Choi Richard Simmerman Yihong Chen Yanan He Ruixue Wang Raquel Godoy-Ruiz Harlan King Gregory Custer D. Travis Gallagher David A. Rozak Melani Solomon Silvia Muro David J. Weber John Orban Thomas R. Fuerst Philip N. Bryan
author_sort	Yingwei Chen
title	Engineering subtilisin proteases that specifically degrade active RAS
title_short	Engineering subtilisin proteases that specifically degrade active RAS
title_full	Engineering subtilisin proteases that specifically degrade active RAS
title_fullStr	Engineering subtilisin proteases that specifically degrade active RAS
title_full_unstemmed	Engineering subtilisin proteases that specifically degrade active RAS
title_sort	engineering subtilisin proteases that specifically degrade active ras
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/742c1a3e0584400b8b631c6e4a1b0ff6
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Engineering subtilisin proteases that specifically degrade active RAS

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