Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia

B-cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) are members of the tumor necrosis factor superfamily of cytokines and can induce B cell activation, differentiation, and antibody production via interaction with their receptors, including transmembrane activator, calcium mo...

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Autores principales:	Wael Alturaiki, Ayman Mubarak, Sajad Ahmad Mir, Adnan Afridi, Mariappan Premanathan, Suresh Mickymaray, Rajendran Vijayakumar, Suliman A. Alsagaby, Sami G. Almalki, Fayez Alghofaili, Ahmad K. Alnemare, Brian F. Flanagan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Asthma IgE B cell BAFF APRIL Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/743e07eb15f3482aad46de745f21ea6c
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spelling	oai:doaj.org-article:743e07eb15f3482aad46de745f21ea6c2021-11-20T04:57:05ZPlasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia1319-562X10.1016/j.sjbs.2021.08.044https://doaj.org/article/743e07eb15f3482aad46de745f21ea6c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21007324https://doaj.org/toc/1319-562XB-cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) are members of the tumor necrosis factor superfamily of cytokines and can induce B cell activation, differentiation, and antibody production via interaction with their receptors, including transmembrane activator, calcium modulator, and cyclophilin ligand interactor (TACI), B-cell maturation antigen (BCMA), and B-cell activating factor receptor (BAFF-R). Herein, we assessed the plasma protein levels of BAFF and APRIL in patients with asthma to determine whether their expression is correlated with total IgE production and examined the surface expression of BAFF/APRIL receptors on B cells. Blood samples were collected from 47 patients with controlled asthma symptoms and 20 healthy normal controls, and plasma levels of APRIL, BAFF, and total IgE protein were quantified by corresponding ELISA assays. Furthermore, lymphocytes were isolated and B cells were analyzed for the presence of BAFF-R, BCMA, and TACI receptors using flow cytometry. Our results showed that IgE, BAFF, and APRIL plasma levels were markedly increased in patients with asthma compared with healthy controls. Moreover, expression of BAFF-R and BCMA, but not that of TACI, was significantly increased in patients with asthma compared with healthy controls. Overall, the findings suggest BAFF and APRIL as key mediators of asthma, and determination of their plasma levels may be useful in monitoring asthma symptoms and treatment response.Wael AlturaikiAyman MubarakSajad Ahmad MirAdnan AfridiMariappan PremanathanSuresh MickymarayRajendran VijayakumarSuliman A. AlsagabySami G. AlmalkiFayez AlghofailiAhmad K. AlnemareBrian F. FlanaganElsevierarticleAsthmaIgEB cellBAFFAPRILBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7455-7459 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Asthma IgE B cell BAFF APRIL Biology (General) QH301-705.5
spellingShingle	Asthma IgE B cell BAFF APRIL Biology (General) QH301-705.5 Wael Alturaiki Ayman Mubarak Sajad Ahmad Mir Adnan Afridi Mariappan Premanathan Suresh Mickymaray Rajendran Vijayakumar Suliman A. Alsagaby Sami G. Almalki Fayez Alghofaili Ahmad K. Alnemare Brian F. Flanagan Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
description	B-cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) are members of the tumor necrosis factor superfamily of cytokines and can induce B cell activation, differentiation, and antibody production via interaction with their receptors, including transmembrane activator, calcium modulator, and cyclophilin ligand interactor (TACI), B-cell maturation antigen (BCMA), and B-cell activating factor receptor (BAFF-R). Herein, we assessed the plasma protein levels of BAFF and APRIL in patients with asthma to determine whether their expression is correlated with total IgE production and examined the surface expression of BAFF/APRIL receptors on B cells. Blood samples were collected from 47 patients with controlled asthma symptoms and 20 healthy normal controls, and plasma levels of APRIL, BAFF, and total IgE protein were quantified by corresponding ELISA assays. Furthermore, lymphocytes were isolated and B cells were analyzed for the presence of BAFF-R, BCMA, and TACI receptors using flow cytometry. Our results showed that IgE, BAFF, and APRIL plasma levels were markedly increased in patients with asthma compared with healthy controls. Moreover, expression of BAFF-R and BCMA, but not that of TACI, was significantly increased in patients with asthma compared with healthy controls. Overall, the findings suggest BAFF and APRIL as key mediators of asthma, and determination of their plasma levels may be useful in monitoring asthma symptoms and treatment response.
format	article
author	Wael Alturaiki Ayman Mubarak Sajad Ahmad Mir Adnan Afridi Mariappan Premanathan Suresh Mickymaray Rajendran Vijayakumar Suliman A. Alsagaby Sami G. Almalki Fayez Alghofaili Ahmad K. Alnemare Brian F. Flanagan
author_facet	Wael Alturaiki Ayman Mubarak Sajad Ahmad Mir Adnan Afridi Mariappan Premanathan Suresh Mickymaray Rajendran Vijayakumar Suliman A. Alsagaby Sami G. Almalki Fayez Alghofaili Ahmad K. Alnemare Brian F. Flanagan
author_sort	Wael Alturaiki
title	Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
title_short	Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
title_full	Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
title_fullStr	Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
title_full_unstemmed	Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia
title_sort	plasma levels of baff and april are elevated in patients with asthma in saudi arabia
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/743e07eb15f3482aad46de745f21ea6c
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Plasma levels of BAFF and APRIL are elevated in patients with asthma in Saudi Arabia

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