Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Ibrahim Birma Bwatanglang,1,2 Faruq Mohammad,3 Nor Azah Yusof,1,3 Jaafar Abdullah,1 Mohd Zobir Hussein,3 Noorjahan Banu Alitheen,4 Nadiah Abu4 1Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Department of Chemistry, Faculty of Science,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bwatanglang IB, Mohammad F, Yusof NA, Abdullah J, Hussein MZ, Alitheen NB, Abu N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Mn-ZnS quantum dots theranostics folic acid cancer diagnosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7448bfacae3e486ab0b0b9ce438fad69
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7448bfacae3e486ab0b0b9ce438fad69
record_format	dspace
spelling	oai:doaj.org-article:7448bfacae3e486ab0b0b9ce438fad692021-12-02T06:31:47ZFolic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy1178-2013https://doaj.org/article/7448bfacae3e486ab0b0b9ce438fad692016-01-01T00:00:00Zhttps://www.dovepress.com/folic-acid-targeted-mnzns-quantum-dots-for-theranostic-applications-of-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ibrahim Birma Bwatanglang,1,2 Faruq Mohammad,3 Nor Azah Yusof,1,3 Jaafar Abdullah,1 Mohd Zobir Hussein,3 Noorjahan Banu Alitheen,4 Nadiah Abu4 1Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Department of Chemistry, Faculty of Science, Adamawa State University, Mubi, Nigeria; 3Institute of Advanced Technology, 4Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: In this study, we synthesized a multifunctional nanoparticulate system with specific targeting, imaging, and drug delivering functionalities by following a three-step protocol that operates at room temperature and solely in aqueous media. The synthesis involves the encapsulation of luminescent Mn:ZnS quantum dots (QDs) with chitosan not only as a stabilizer in biological environment, but also to further provide active binding sites for the conjugation of other biomolecules. Folic acid was incorporated as targeting agent for the specific targeting of the nanocarrier toward the cells overexpressing folate receptors. Thus, the formed composite emits orange–red fluorescence around 600 nm and investigated to the highest intensity at Mn2+ doping concentration of 15 at.% and relatively more stable at low acidic and low alkaline pH levels. The structural characteristics and optical properties were thoroughly analyzed by using Fourier transform infrared, X-ray diffraction, dynamic light scattering, ultraviolet-visible, and fluorescence spectroscopy. Further characterization was conducted using thermogravimetric analysis, high-resolution transmission electron microscopy, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence, and X-ray photoelectron spectroscopy. The cell viability and proliferation studies by means of MTT assay have demonstrated that the as-synthesized composites do not exhibit any toxicity toward the human breast cell line MCF-10 (noncancer) and the breast cancer cell lines (MCF-7 and MDA-MB-231) up to a 500 µg/mL concentration. The cellular uptake of the nanocomposites was assayed by confocal laser scanning microscope by taking advantage of the conjugated Mn:ZnS QDs as fluorescence makers. The result showed that the functionalization of the chitosan-encapsulated QDs with folic acid enhanced the internalization and binding affinity of the nanocarrier toward folate receptor-overexpressed cells. Therefore, we hypothesized that due to the nontoxic nature of the composite, the as-synthesized nanoparticulate system can be used as a promising candidate for theranostic applications, especially for a simultaneous targeted drug delivery and cellular imaging. Keywords: Mn:ZnS, quantum dots, theranostics, folic acid, cancer diagnosis, fluorescence imagingBwatanglang IBMohammad FYusof NAAbdullah JHussein MZAlitheen NBAbu NDove Medical PressarticleMn-ZnSquantum dotstheranosticsfolic acidcancer diagnosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 413-428 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mn-ZnS quantum dots theranostics folic acid cancer diagnosis Medicine (General) R5-920
spellingShingle	Mn-ZnS quantum dots theranostics folic acid cancer diagnosis Medicine (General) R5-920 Bwatanglang IB Mohammad F Yusof NA Abdullah J Hussein MZ Alitheen NB Abu N Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy
description	Ibrahim Birma Bwatanglang,1,2 Faruq Mohammad,3 Nor Azah Yusof,1,3 Jaafar Abdullah,1 Mohd Zobir Hussein,3 Noorjahan Banu Alitheen,4 Nadiah Abu4 1Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Department of Chemistry, Faculty of Science, Adamawa State University, Mubi, Nigeria; 3Institute of Advanced Technology, 4Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: In this study, we synthesized a multifunctional nanoparticulate system with specific targeting, imaging, and drug delivering functionalities by following a three-step protocol that operates at room temperature and solely in aqueous media. The synthesis involves the encapsulation of luminescent Mn:ZnS quantum dots (QDs) with chitosan not only as a stabilizer in biological environment, but also to further provide active binding sites for the conjugation of other biomolecules. Folic acid was incorporated as targeting agent for the specific targeting of the nanocarrier toward the cells overexpressing folate receptors. Thus, the formed composite emits orange–red fluorescence around 600 nm and investigated to the highest intensity at Mn2+ doping concentration of 15 at.% and relatively more stable at low acidic and low alkaline pH levels. The structural characteristics and optical properties were thoroughly analyzed by using Fourier transform infrared, X-ray diffraction, dynamic light scattering, ultraviolet-visible, and fluorescence spectroscopy. Further characterization was conducted using thermogravimetric analysis, high-resolution transmission electron microscopy, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence, and X-ray photoelectron spectroscopy. The cell viability and proliferation studies by means of MTT assay have demonstrated that the as-synthesized composites do not exhibit any toxicity toward the human breast cell line MCF-10 (noncancer) and the breast cancer cell lines (MCF-7 and MDA-MB-231) up to a 500 µg/mL concentration. The cellular uptake of the nanocomposites was assayed by confocal laser scanning microscope by taking advantage of the conjugated Mn:ZnS QDs as fluorescence makers. The result showed that the functionalization of the chitosan-encapsulated QDs with folic acid enhanced the internalization and binding affinity of the nanocarrier toward folate receptor-overexpressed cells. Therefore, we hypothesized that due to the nontoxic nature of the composite, the as-synthesized nanoparticulate system can be used as a promising candidate for theranostic applications, especially for a simultaneous targeted drug delivery and cellular imaging. Keywords: Mn:ZnS, quantum dots, theranostics, folic acid, cancer diagnosis, fluorescence imaging
format	article
author	Bwatanglang IB Mohammad F Yusof NA Abdullah J Hussein MZ Alitheen NB Abu N
author_facet	Bwatanglang IB Mohammad F Yusof NA Abdullah J Hussein MZ Alitheen NB Abu N
author_sort	Bwatanglang IB
title	Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy
title_short	Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy
title_full	Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy
title_fullStr	Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy
title_full_unstemmed	Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy
title_sort	folic acid targeted mn:zns quantum dots for theranostic applications of cancer cell imaging and therapy
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/7448bfacae3e486ab0b0b9ce438fad69
work_keys_str_mv	AT bwatanglangib folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy AT mohammadf folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy AT yusofna folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy AT abdullahj folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy AT husseinmz folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy AT alitheennb folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy AT abun folicacidtargetedmnznsquantumdotsfortheranosticapplicationsofcancercellimagingandtherapy
_version_	1718399877327618048

Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Ejemplares similares