Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress

Drought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of <i>Lolium temulentum</i> exposed to droug...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ruth C. Martin, Brent A. Kronmiller, James E. Dombrowski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	abiotic stress drought stress heat stress hormones <i>Lolium temulentum</i> RNA-sequencing Botany QK1-989
Acceso en línea:	https://doaj.org/article/74540539b6684c969b1736694e13ed5a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:74540539b6684c969b1736694e13ed5a
record_format	dspace
spelling	oai:doaj.org-article:74540539b6684c969b1736694e13ed5a2021-11-25T18:44:10ZTranscriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress10.3390/plants101122472223-7747https://doaj.org/article/74540539b6684c969b1736694e13ed5a2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2247https://doaj.org/toc/2223-7747Drought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of <i>Lolium temulentum</i> exposed to drought plus heat and non-stressed control plants revealed 20,221 unique up-regulated and 17,034 unique down-regulated differentially regulated transcripts. Gene ontology analysis revealed a strong emphasis on transcriptional regulation, protein folding, cell cycle/parts, organelles, binding, transport, signaling, oxidoreductase, and antioxidant activity. Differentially expressed genes (DEGs) encoding for transcriptional control proteins such as basic leucine zipper, APETALA2/Ethylene Responsive Factor, NAC, and WRKY transcription factors, and Zinc Finger (CCCH type and others) proteins were more often up-regulated, while DEGs encoding Basic Helix-Loop-Helix, MYB and GATA transcription factors, and C2H2 type Zinc Finger proteins were more often down-regulated. The DEGs encoding heat shock transcription factors were only up-regulated. Of the hormones, auxin-related DEGs were the most prevalent, encoding for auxin response factors, binding proteins, and efflux/influx carriers. Gibberellin-, cytokinin- and ABA-related DEGs were also prevalent, with fewer DEGs related to jasmonates and brassinosteroids. Knowledge of genes/pathways that grasses use to respond to the combination of heat/drought will be useful in developing multi-stress resistant grasses.Ruth C. MartinBrent A. KronmillerJames E. DombrowskiMDPI AGarticleabiotic stressdrought stressheat stresshormones<i>Lolium temulentum</i>RNA-sequencingBotanyQK1-989ENPlants, Vol 10, Iss 2247, p 2247 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	abiotic stress drought stress heat stress hormones <i>Lolium temulentum</i> RNA-sequencing Botany QK1-989
spellingShingle	abiotic stress drought stress heat stress hormones <i>Lolium temulentum</i> RNA-sequencing Botany QK1-989 Ruth C. Martin Brent A. Kronmiller James E. Dombrowski Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
description	Drought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of <i>Lolium temulentum</i> exposed to drought plus heat and non-stressed control plants revealed 20,221 unique up-regulated and 17,034 unique down-regulated differentially regulated transcripts. Gene ontology analysis revealed a strong emphasis on transcriptional regulation, protein folding, cell cycle/parts, organelles, binding, transport, signaling, oxidoreductase, and antioxidant activity. Differentially expressed genes (DEGs) encoding for transcriptional control proteins such as basic leucine zipper, APETALA2/Ethylene Responsive Factor, NAC, and WRKY transcription factors, and Zinc Finger (CCCH type and others) proteins were more often up-regulated, while DEGs encoding Basic Helix-Loop-Helix, MYB and GATA transcription factors, and C2H2 type Zinc Finger proteins were more often down-regulated. The DEGs encoding heat shock transcription factors were only up-regulated. Of the hormones, auxin-related DEGs were the most prevalent, encoding for auxin response factors, binding proteins, and efflux/influx carriers. Gibberellin-, cytokinin- and ABA-related DEGs were also prevalent, with fewer DEGs related to jasmonates and brassinosteroids. Knowledge of genes/pathways that grasses use to respond to the combination of heat/drought will be useful in developing multi-stress resistant grasses.
format	article
author	Ruth C. Martin Brent A. Kronmiller James E. Dombrowski
author_facet	Ruth C. Martin Brent A. Kronmiller James E. Dombrowski
author_sort	Ruth C. Martin
title	Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
title_short	Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
title_full	Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
title_fullStr	Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
title_full_unstemmed	Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress
title_sort	transcriptome analysis of <i>lolium temulentum</i> exposed to a combination of drought and heat stress
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/74540539b6684c969b1736694e13ed5a
work_keys_str_mv	AT ruthcmartin transcriptomeanalysisofiloliumtemulentumiexposedtoacombinationofdroughtandheatstress AT brentakronmiller transcriptomeanalysisofiloliumtemulentumiexposedtoacombinationofdroughtandheatstress AT jamesedombrowski transcriptomeanalysisofiloliumtemulentumiexposedtoacombinationofdroughtandheatstress
_version_	1718410780059107328

Transcriptome Analysis of <i>Lolium temulentum</i> Exposed to a Combination of Drought and Heat Stress

Ejemplares similares