Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.

The nickel resistance determinant ncrABCY was identified in Leptospirillum ferriphilum UBK03. Within this operon, ncrA and ncrC encode two membrane proteins that form an efflux system, and ncrB encodes NcrB, which belongs to an uncharacterized family (DUF156) of proteins. How this determinant is reg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tao Zhu, Jian Tian, Shuangyu Zhang, Ningfeng Wu, Yunliu Fan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7456db41dc284b8cbecc4810015a89bc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7456db41dc284b8cbecc4810015a89bc
record_format	dspace
spelling	oai:doaj.org-article:7456db41dc284b8cbecc4810015a89bc2021-11-18T06:58:01ZIdentification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.1932-620310.1371/journal.pone.0017367https://doaj.org/article/7456db41dc284b8cbecc4810015a89bc2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21387010/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The nickel resistance determinant ncrABCY was identified in Leptospirillum ferriphilum UBK03. Within this operon, ncrA and ncrC encode two membrane proteins that form an efflux system, and ncrB encodes NcrB, which belongs to an uncharacterized family (DUF156) of proteins. How this determinant is regulated remains unknown. Our data indicate that expression of the nickel resistance determinant is induced by nickel. The promoter of ncrA, designated pncrA, was cloned into the promoter probe vector pPR9TT, and co-transformed with either a wild-type or mutant nickel resistance determinant. The results revealed that ncrB encoded a transcriptional regulator that could regulate the expression of ncrA, ncrB, and ncrC. A GC-rich inverted repeat sequence was identified in the promoter pncrA. Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro; this was confirmed by bacterial one-hybrid analysis. Moreover, this binding was inhibited in the presence of nickel ions. Thus, we classified NcrB as a transcriptional regulator that recognizes the inverted repeat sequence binding motif to regulate the expression of the key nickel resistance gene, ncrA.Tao ZhuJian TianShuangyu ZhangNingfeng WuYunliu FanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17367 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Zhu Jian Tian Shuangyu Zhang Ningfeng Wu Yunliu Fan Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
description	The nickel resistance determinant ncrABCY was identified in Leptospirillum ferriphilum UBK03. Within this operon, ncrA and ncrC encode two membrane proteins that form an efflux system, and ncrB encodes NcrB, which belongs to an uncharacterized family (DUF156) of proteins. How this determinant is regulated remains unknown. Our data indicate that expression of the nickel resistance determinant is induced by nickel. The promoter of ncrA, designated pncrA, was cloned into the promoter probe vector pPR9TT, and co-transformed with either a wild-type or mutant nickel resistance determinant. The results revealed that ncrB encoded a transcriptional regulator that could regulate the expression of ncrA, ncrB, and ncrC. A GC-rich inverted repeat sequence was identified in the promoter pncrA. Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro; this was confirmed by bacterial one-hybrid analysis. Moreover, this binding was inhibited in the presence of nickel ions. Thus, we classified NcrB as a transcriptional regulator that recognizes the inverted repeat sequence binding motif to regulate the expression of the key nickel resistance gene, ncrA.
format	article
author	Tao Zhu Jian Tian Shuangyu Zhang Ningfeng Wu Yunliu Fan
author_facet	Tao Zhu Jian Tian Shuangyu Zhang Ningfeng Wu Yunliu Fan
author_sort	Tao Zhu
title	Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
title_short	Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
title_full	Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
title_fullStr	Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
title_full_unstemmed	Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
title_sort	identification of the transcriptional regulator ncrb in the nickel resistance determinant of leptospirillum ferriphilum ubk03.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7456db41dc284b8cbecc4810015a89bc
work_keys_str_mv	AT taozhu identificationofthetranscriptionalregulatorncrbinthenickelresistancedeterminantofleptospirillumferriphilumubk03 AT jiantian identificationofthetranscriptionalregulatorncrbinthenickelresistancedeterminantofleptospirillumferriphilumubk03 AT shuangyuzhang identificationofthetranscriptionalregulatorncrbinthenickelresistancedeterminantofleptospirillumferriphilumubk03 AT ningfengwu identificationofthetranscriptionalregulatorncrbinthenickelresistancedeterminantofleptospirillumferriphilumubk03 AT yunliufan identificationofthetranscriptionalregulatorncrbinthenickelresistancedeterminantofleptospirillumferriphilumubk03
_version_	1718424110272348160

Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.

Ejemplares similares