Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus

Abstract Marek’s disease virus (MDV) induces severe immunosuppression and lymphomagenesis in the chicken, its natural host, and results in a condition that investigated the pathogenesis of MDV and have begun to focus on the expression profiling of circular RNAs (circRNAs). However, little is known a...

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Autores principales:	Aijun Sun, Rui Wang, Shuaikang Yang, Xiaojing Zhu, Ying Liu, Man Teng, Luping Zheng, Jun Luo, Gaiping Zhang, Guoqing Zhuang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7456fc8f821f4c869624c6cf4bd9b6b8
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spelling	oai:doaj.org-article:7456fc8f821f4c869624c6cf4bd9b6b82021-12-02T14:49:10ZComprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus10.1038/s41598-021-90548-12045-2322https://doaj.org/article/7456fc8f821f4c869624c6cf4bd9b6b82021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90548-1https://doaj.org/toc/2045-2322Abstract Marek’s disease virus (MDV) induces severe immunosuppression and lymphomagenesis in the chicken, its natural host, and results in a condition that investigated the pathogenesis of MDV and have begun to focus on the expression profiling of circular RNAs (circRNAs). However, little is known about how the expression of circRNAs is referred to as Marek’s disease. Previous reports have is regulated during MDV replication. Here, we carried out a comprehensive profiling analysis of N6-methyladenosine (m6A) modification on the circRNA transcriptome in infected and uninfected chicken embryonic fibroblast (CEF) cells. Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) revealed that m6A modification was highly conserved in circRNAs. Comparing to the uninfected group, the number of peaks and conserved motifs were not significantly different in cells that were infected with MDV, although reduced abundance of circRNA m6A modifications. However, gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses revealed that the insulin signaling pathway was associated with the regulation of m6A modified circRNAs in MDV infection. This is the first report to describe alterations in the transcriptome-wide profiling of m6A modified circRNAs in MDV-infected CEF cells.Aijun SunRui WangShuaikang YangXiaojing ZhuYing LiuMan TengLuping ZhengJun LuoGaiping ZhangGuoqing ZhuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aijun Sun Rui Wang Shuaikang Yang Xiaojing Zhu Ying Liu Man Teng Luping Zheng Jun Luo Gaiping Zhang Guoqing Zhuang Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus
description	Abstract Marek’s disease virus (MDV) induces severe immunosuppression and lymphomagenesis in the chicken, its natural host, and results in a condition that investigated the pathogenesis of MDV and have begun to focus on the expression profiling of circular RNAs (circRNAs). However, little is known about how the expression of circRNAs is referred to as Marek’s disease. Previous reports have is regulated during MDV replication. Here, we carried out a comprehensive profiling analysis of N6-methyladenosine (m6A) modification on the circRNA transcriptome in infected and uninfected chicken embryonic fibroblast (CEF) cells. Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) revealed that m6A modification was highly conserved in circRNAs. Comparing to the uninfected group, the number of peaks and conserved motifs were not significantly different in cells that were infected with MDV, although reduced abundance of circRNA m6A modifications. However, gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses revealed that the insulin signaling pathway was associated with the regulation of m6A modified circRNAs in MDV infection. This is the first report to describe alterations in the transcriptome-wide profiling of m6A modified circRNAs in MDV-infected CEF cells.
format	article
author	Aijun Sun Rui Wang Shuaikang Yang Xiaojing Zhu Ying Liu Man Teng Luping Zheng Jun Luo Gaiping Zhang Guoqing Zhuang
author_facet	Aijun Sun Rui Wang Shuaikang Yang Xiaojing Zhu Ying Liu Man Teng Luping Zheng Jun Luo Gaiping Zhang Guoqing Zhuang
author_sort	Aijun Sun
title	Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus
title_short	Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus
title_full	Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus
title_fullStr	Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus
title_full_unstemmed	Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus
title_sort	comprehensive profiling analysis of the n6-methyladenosine-modified circular rna transcriptome in cultured cells infected with marek’s disease virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7456fc8f821f4c869624c6cf4bd9b6b8
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Comprehensive profiling analysis of the N6-methyladenosine-modified circular RNA transcriptome in cultured cells infected with Marek’s disease virus

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