Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT

BK polyomavirus (BKPyV) persists lifelong in renal and urothelial cells with asymptomatic urinary shedding in healthy individuals. In some immunocompromised persons after transplantation of hematopoietic stem cells (HSCT), the BKPyV high-rate replication is associated with haemorrhagic cystitis (HC)...

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Autores principales:	Markéta Šťastná-Marková, Eva Hamšíková, Petr Hainz, Petr Hubáček, Marie Kroutilová, Jitka Kryštofová, Viera Ludvíková, Jan Musil, Pavla Pecherková, Martina Saláková, Vojtěch Šroller, Jan Vydra, Šárka Němečková
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	human BK polyomavirus 1 and 4 (BKPyV 1 and 4) haemorrhagic cystitis anti/BKPyV IgG T-cell response VP1 LTag Medicine R
Acceso en línea:	https://doaj.org/article/7460f6432f664b1c8d82438383122ca2
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spelling	oai:doaj.org-article:7460f6432f664b1c8d82438383122ca22021-11-25T19:10:14ZPretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT10.3390/vaccines91112262076-393Xhttps://doaj.org/article/7460f6432f664b1c8d82438383122ca22021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1226https://doaj.org/toc/2076-393XBK polyomavirus (BKPyV) persists lifelong in renal and urothelial cells with asymptomatic urinary shedding in healthy individuals. In some immunocompromised persons after transplantation of hematopoietic stem cells (HSCT), the BKPyV high-rate replication is associated with haemorrhagic cystitis (HC). We tested whether the status of BKPyV immunity prior to HSCT could provide evidence for the BKPyV tendency to reactivate. We have shown that measurement of pretransplant anti-BKPyV 1 and 4 IgG levels can be used to evaluate the HC risk. Patients with anti-BKPyV IgG in the range of the 1st–2nd quartile of positive values and with positive clinical risk markers have a significantly increased HC risk, in comparison to the reference group of patients with “non-reactive” anti-BKPyV IgG levels and with low clinical risk (LCR) (<i>p</i> = 0.0009). The predictive value of pretransplant BKPyV-specific IgG was confirmed by determination of genotypes of the shed virus. A positive predictive value was also found for pretransplant T-cell immunity to the BKPyV antigen VP1 because the magnitude of IFN-γ T-cell response inversely correlated with posttransplant DNAuria and with HC. Our novel data suggest that specific T-cells control BKPyV latency before HSCT, and in this way may influence BKPyV reactivation after HSCT. Our study has shown that prediction using a combination of clinical and immunological pretransplant risk factors can help early identification of HSCT recipients at high risk of BKPyV disease.Markéta Šťastná-MarkováEva HamšíkováPetr HainzPetr HubáčekMarie KroutilováJitka KryštofováViera LudvíkováJan MusilPavla PecherkováMartina SalákováVojtěch ŠrollerJan VydraŠárka NěmečkováMDPI AGarticlehuman BK polyomavirus 1 and 4 (BKPyV 1 and 4)haemorrhagic cystitisanti/BKPyV IgGT-cell responseVP1LTagMedicineRENVaccines, Vol 9, Iss 1226, p 1226 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human BK polyomavirus 1 and 4 (BKPyV 1 and 4) haemorrhagic cystitis anti/BKPyV IgG T-cell response VP1 LTag Medicine R
spellingShingle	human BK polyomavirus 1 and 4 (BKPyV 1 and 4) haemorrhagic cystitis anti/BKPyV IgG T-cell response VP1 LTag Medicine R Markéta Šťastná-Marková Eva Hamšíková Petr Hainz Petr Hubáček Marie Kroutilová Jitka Kryštofová Viera Ludvíková Jan Musil Pavla Pecherková Martina Saláková Vojtěch Šroller Jan Vydra Šárka Němečková Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT
description	BK polyomavirus (BKPyV) persists lifelong in renal and urothelial cells with asymptomatic urinary shedding in healthy individuals. In some immunocompromised persons after transplantation of hematopoietic stem cells (HSCT), the BKPyV high-rate replication is associated with haemorrhagic cystitis (HC). We tested whether the status of BKPyV immunity prior to HSCT could provide evidence for the BKPyV tendency to reactivate. We have shown that measurement of pretransplant anti-BKPyV 1 and 4 IgG levels can be used to evaluate the HC risk. Patients with anti-BKPyV IgG in the range of the 1st–2nd quartile of positive values and with positive clinical risk markers have a significantly increased HC risk, in comparison to the reference group of patients with “non-reactive” anti-BKPyV IgG levels and with low clinical risk (LCR) (<i>p</i> = 0.0009). The predictive value of pretransplant BKPyV-specific IgG was confirmed by determination of genotypes of the shed virus. A positive predictive value was also found for pretransplant T-cell immunity to the BKPyV antigen VP1 because the magnitude of IFN-γ T-cell response inversely correlated with posttransplant DNAuria and with HC. Our novel data suggest that specific T-cells control BKPyV latency before HSCT, and in this way may influence BKPyV reactivation after HSCT. Our study has shown that prediction using a combination of clinical and immunological pretransplant risk factors can help early identification of HSCT recipients at high risk of BKPyV disease.
format	article
author	Markéta Šťastná-Marková Eva Hamšíková Petr Hainz Petr Hubáček Marie Kroutilová Jitka Kryštofová Viera Ludvíková Jan Musil Pavla Pecherková Martina Saláková Vojtěch Šroller Jan Vydra Šárka Němečková
author_facet	Markéta Šťastná-Marková Eva Hamšíková Petr Hainz Petr Hubáček Marie Kroutilová Jitka Kryštofová Viera Ludvíková Jan Musil Pavla Pecherková Martina Saláková Vojtěch Šroller Jan Vydra Šárka Němečková
author_sort	Markéta Šťastná-Marková
title	Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT
title_short	Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT
title_full	Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT
title_fullStr	Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT
title_full_unstemmed	Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT
title_sort	pretransplant bk virus-specific t-cell-mediated immunity and serotype specific antibodies may have utility in identifying patients at risk of bk virus-associated haemorrhagic cystitis after allogeneic hsct
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7460f6432f664b1c8d82438383122ca2
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Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cystitis after Allogeneic HSCT

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