An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70

Background: Pumpkins (Cucurbita pepo L.) were described to have antioxidant, anti-inflammatory, anti-fatigue, and antidepressant-like effect. The adrenal gland is an important stress-responsive organ that maintains homeostasis during stress.Objectives: This study aimed to assess the efficacy of the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hailah M. Almohaimeed, Shereen Hamed, Hanan S. Seleem, Ashwaq H. Batawi, Zuhair M. Mohammedsaleh, Maha Jameal Balgoon, Soad S. Ali, Soad Al Jaouni, Nasra Ayuob
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	pumpkin stress depression caspase-3 ki67 Hsp70 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/746324fdcdad41eab90a5d96df711cf7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:746324fdcdad41eab90a5d96df711cf7
record_format	dspace
spelling	oai:doaj.org-article:746324fdcdad41eab90a5d96df711cf72021-11-10T07:46:24ZAn Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP701663-981210.3389/fphar.2021.749766https://doaj.org/article/746324fdcdad41eab90a5d96df711cf72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.749766/fullhttps://doaj.org/toc/1663-9812Background: Pumpkins (Cucurbita pepo L.) were described to have antioxidant, anti-inflammatory, anti-fatigue, and antidepressant-like effect. The adrenal gland is an important stress-responsive organ that maintains homeostasis during stress.Objectives: This study aimed to assess the efficacy of the administration of Cucurbita pepo L. (CP) extract in relieving behavioral, biochemical, and structural changes in the adrenal gland induced by exposure to chronic unpredictable mild stress (CUMS) and to explore the mechanism behind this impact.Materials and Methods: Forty male albino rats were divided into 4 groups (n = 10): control, CUMS, fluoxetine-treated, and CP-treated groups. Behavioral changes, corticosterone level, pro-inflammatory cytokines TNF-α and IL-6, and oxidant/antioxidant profile were assessed in the serum at the end of the experiment. Adrenal glands were processed for histopathological and immunohistochemical assessment. Gene expression of caspase-3 and Ki67 and heat shock protein 70 (HSP70) were assessed in adrenal glands using RT-PCR.Results: The CP extract significantly reduced the corticosterone level (p < 0.001), immobility time (p < 0.001), and inflammatory and oxidative changes associated with CUMS-induced depression compared to the untreated group. The CP extract alleviated CUMS-induced adrenal histopathological changes and significantly reduced apoptosis (p < 0.001) and significantly upregulated antioxidant levels in the serum.Conclusion:Cucurbita pepo L. effectively ameliorated the chronic stress-induced behavioral, biochemical, and adrenal structural changes mostly through its antioxidant and anti-inflammatory effects.Hailah M. AlmohaimeedShereen HamedHanan S. SeleemHanan S. SeleemAshwaq H. BatawiZuhair M. MohammedsalehMaha Jameal BalgoonSoad S. AliSoad S. AliSoad Al JaouniSoad Al JaouniNasra AyuobFrontiers Media S.A.articlepumpkinstressdepressioncaspase-3ki67Hsp70Therapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pumpkin stress depression caspase-3 ki67 Hsp70 Therapeutics. Pharmacology RM1-950
spellingShingle	pumpkin stress depression caspase-3 ki67 Hsp70 Therapeutics. Pharmacology RM1-950 Hailah M. Almohaimeed Shereen Hamed Hanan S. Seleem Hanan S. Seleem Ashwaq H. Batawi Zuhair M. Mohammedsaleh Maha Jameal Balgoon Soad S. Ali Soad S. Ali Soad Al Jaouni Soad Al Jaouni Nasra Ayuob An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70
description	Background: Pumpkins (Cucurbita pepo L.) were described to have antioxidant, anti-inflammatory, anti-fatigue, and antidepressant-like effect. The adrenal gland is an important stress-responsive organ that maintains homeostasis during stress.Objectives: This study aimed to assess the efficacy of the administration of Cucurbita pepo L. (CP) extract in relieving behavioral, biochemical, and structural changes in the adrenal gland induced by exposure to chronic unpredictable mild stress (CUMS) and to explore the mechanism behind this impact.Materials and Methods: Forty male albino rats were divided into 4 groups (n = 10): control, CUMS, fluoxetine-treated, and CP-treated groups. Behavioral changes, corticosterone level, pro-inflammatory cytokines TNF-α and IL-6, and oxidant/antioxidant profile were assessed in the serum at the end of the experiment. Adrenal glands were processed for histopathological and immunohistochemical assessment. Gene expression of caspase-3 and Ki67 and heat shock protein 70 (HSP70) were assessed in adrenal glands using RT-PCR.Results: The CP extract significantly reduced the corticosterone level (p < 0.001), immobility time (p < 0.001), and inflammatory and oxidative changes associated with CUMS-induced depression compared to the untreated group. The CP extract alleviated CUMS-induced adrenal histopathological changes and significantly reduced apoptosis (p < 0.001) and significantly upregulated antioxidant levels in the serum.Conclusion:Cucurbita pepo L. effectively ameliorated the chronic stress-induced behavioral, biochemical, and adrenal structural changes mostly through its antioxidant and anti-inflammatory effects.
format	article
author	Hailah M. Almohaimeed Shereen Hamed Hanan S. Seleem Hanan S. Seleem Ashwaq H. Batawi Zuhair M. Mohammedsaleh Maha Jameal Balgoon Soad S. Ali Soad S. Ali Soad Al Jaouni Soad Al Jaouni Nasra Ayuob
author_facet	Hailah M. Almohaimeed Shereen Hamed Hanan S. Seleem Hanan S. Seleem Ashwaq H. Batawi Zuhair M. Mohammedsaleh Maha Jameal Balgoon Soad S. Ali Soad S. Ali Soad Al Jaouni Soad Al Jaouni Nasra Ayuob
author_sort	Hailah M. Almohaimeed
title	An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70
title_short	An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70
title_full	An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70
title_fullStr	An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70
title_full_unstemmed	An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70
title_sort	ethanolic extract of cucurbita pepo l. seeds modifies neuroendocrine disruption in chronic stressed rats and adrenal expression of inflammatory markers and hsp70
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/746324fdcdad41eab90a5d96df711cf7
work_keys_str_mv	AT hailahmalmohaimeed anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT shereenhamed anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT hanansseleem anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT hanansseleem anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT ashwaqhbatawi anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT zuhairmmohammedsaleh anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT mahajamealbalgoon anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadsali anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadsali anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadaljaouni anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadaljaouni anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT nasraayuob anethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT hailahmalmohaimeed ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT shereenhamed ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT hanansseleem ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT hanansseleem ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT ashwaqhbatawi ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT zuhairmmohammedsaleh ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT mahajamealbalgoon ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadsali ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadsali ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadaljaouni ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT soadaljaouni ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70 AT nasraayuob ethanolicextractofcucurbitapepolseedsmodifiesneuroendocrinedisruptioninchronicstressedratsandadrenalexpressionofinflammatorymarkersandhsp70
_version_	1718440385376681984

An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70

Ejemplares similares