Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes

Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transform...

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Autores principales: Xuejing Wen, Jingxin Wang, Daoyuan Zhang, Yu Ding, Xiaoyu Ji, Zilong Tan, Yucheng Wang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/746b1199e6d840ef81b4c011a04fce77
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spelling oai:doaj.org-article:746b1199e6d840ef81b4c011a04fce772021-12-02T12:40:39ZReverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes10.1038/s42003-020-01500-42399-3642https://doaj.org/article/746b1199e6d840ef81b4c011a04fce772020-12-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01500-4https://doaj.org/toc/2399-3642Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transformation of the target promoter to increase efficiency.Xuejing WenJingxin WangDaoyuan ZhangYu DingXiaoyu JiZilong TanYucheng WangNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 3, Iss 1, Pp 1-12 (2020)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Xuejing Wen
Jingxin Wang
Daoyuan Zhang
Yu Ding
Xiaoyu Ji
Zilong Tan
Yucheng Wang
Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
description Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transformation of the target promoter to increase efficiency.
format article
author Xuejing Wen
Jingxin Wang
Daoyuan Zhang
Yu Ding
Xiaoyu Ji
Zilong Tan
Yucheng Wang
author_facet Xuejing Wen
Jingxin Wang
Daoyuan Zhang
Yu Ding
Xiaoyu Ji
Zilong Tan
Yucheng Wang
author_sort Xuejing Wen
title Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
title_short Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
title_full Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
title_fullStr Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
title_full_unstemmed Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
title_sort reverse chromatin immunoprecipitation (r-chip) enables investigation of the upstream regulators of plant genes
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/746b1199e6d840ef81b4c011a04fce77
work_keys_str_mv AT xuejingwen reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
AT jingxinwang reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
AT daoyuanzhang reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
AT yuding reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
AT xiaoyuji reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
AT zilongtan reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
AT yuchengwang reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes
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