Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes
Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transform...
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Nature Portfolio
2020
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oai:doaj.org-article:746b1199e6d840ef81b4c011a04fce772021-12-02T12:40:39ZReverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes10.1038/s42003-020-01500-42399-3642https://doaj.org/article/746b1199e6d840ef81b4c011a04fce772020-12-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01500-4https://doaj.org/toc/2399-3642Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transformation of the target promoter to increase efficiency.Xuejing WenJingxin WangDaoyuan ZhangYu DingXiaoyu JiZilong TanYucheng WangNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 3, Iss 1, Pp 1-12 (2020) |
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DOAJ |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Xuejing Wen Jingxin Wang Daoyuan Zhang Yu Ding Xiaoyu Ji Zilong Tan Yucheng Wang Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes |
description |
Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transformation of the target promoter to increase efficiency. |
format |
article |
author |
Xuejing Wen Jingxin Wang Daoyuan Zhang Yu Ding Xiaoyu Ji Zilong Tan Yucheng Wang |
author_facet |
Xuejing Wen Jingxin Wang Daoyuan Zhang Yu Ding Xiaoyu Ji Zilong Tan Yucheng Wang |
author_sort |
Xuejing Wen |
title |
Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes |
title_short |
Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes |
title_full |
Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes |
title_fullStr |
Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes |
title_full_unstemmed |
Reverse Chromatin Immunoprecipitation (R-ChIP) enables investigation of the upstream regulators of plant genes |
title_sort |
reverse chromatin immunoprecipitation (r-chip) enables investigation of the upstream regulators of plant genes |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/746b1199e6d840ef81b4c011a04fce77 |
work_keys_str_mv |
AT xuejingwen reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes AT jingxinwang reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes AT daoyuanzhang reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes AT yuding reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes AT xiaoyuji reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes AT zilongtan reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes AT yuchengwang reversechromatinimmunoprecipitationrchipenablesinvestigationoftheupstreamregulatorsofplantgenes |
_version_ |
1718393744734027776 |