Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.

The mosquito is the obligate vector for malaria transmission. To complete its development within the mosquito, the malaria parasite Plasmodium must overcome the protective action of the mosquito innate immune system. Here we report on the involvement of the Anopheles gambiae orthologue of a conserve...

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Autores principales:	Ryan C Smith, Abraham G Eappen, Andrea J Radtke, Marcelo Jacobs-Lorena
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/746c2005165b400da6c7edb555eb1351
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spelling	oai:doaj.org-article:746c2005165b400da6c7edb555eb13512021-11-18T06:06:27ZRegulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.1553-73661553-737410.1371/journal.ppat.1002965https://doaj.org/article/746c2005165b400da6c7edb555eb13512012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23093936/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The mosquito is the obligate vector for malaria transmission. To complete its development within the mosquito, the malaria parasite Plasmodium must overcome the protective action of the mosquito innate immune system. Here we report on the involvement of the Anopheles gambiae orthologue of a conserved component of the vertebrate immune system, LPS-induced TNFα transcription factor (LITAF), and its role in mosquito anti-Plasmodium immunity. An. gambiae LITAF-like 3 (LL3) expression is up-regulated in response to midgut invasion by both rodent and human malaria parasites. Silencing of LL3 expression greatly increases parasite survival, indicating that LL3 is part of an anti-Plasmodium defense mechanism. Electrophoretic mobility shift assays identified specific LL3 DNA-binding motifs within the promoter of SRPN6, a gene that also mediates mosquito defense against Plasmodium. Further experiments indicated that these motifs play a direct role in LL3 regulation of SRPN6 expression. We conclude that LL3 is a transcription factor capable of modulating SRPN6 expression as part of the mosquito anti-Plasmodium immune response.Ryan C SmithAbraham G EappenAndrea J RadtkeMarcelo Jacobs-LorenaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 10, p e1002965 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Ryan C Smith Abraham G Eappen Andrea J Radtke Marcelo Jacobs-Lorena Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.
description	The mosquito is the obligate vector for malaria transmission. To complete its development within the mosquito, the malaria parasite Plasmodium must overcome the protective action of the mosquito innate immune system. Here we report on the involvement of the Anopheles gambiae orthologue of a conserved component of the vertebrate immune system, LPS-induced TNFα transcription factor (LITAF), and its role in mosquito anti-Plasmodium immunity. An. gambiae LITAF-like 3 (LL3) expression is up-regulated in response to midgut invasion by both rodent and human malaria parasites. Silencing of LL3 expression greatly increases parasite survival, indicating that LL3 is part of an anti-Plasmodium defense mechanism. Electrophoretic mobility shift assays identified specific LL3 DNA-binding motifs within the promoter of SRPN6, a gene that also mediates mosquito defense against Plasmodium. Further experiments indicated that these motifs play a direct role in LL3 regulation of SRPN6 expression. We conclude that LL3 is a transcription factor capable of modulating SRPN6 expression as part of the mosquito anti-Plasmodium immune response.
format	article
author	Ryan C Smith Abraham G Eappen Andrea J Radtke Marcelo Jacobs-Lorena
author_facet	Ryan C Smith Abraham G Eappen Andrea J Radtke Marcelo Jacobs-Lorena
author_sort	Ryan C Smith
title	Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.
title_short	Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.
title_full	Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.
title_fullStr	Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.
title_full_unstemmed	Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.
title_sort	regulation of anti-plasmodium immunity by a litaf-like transcription factor in the malaria vector anopheles gambiae.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/746c2005165b400da6c7edb555eb1351
work_keys_str_mv	AT ryancsmith regulationofantiplasmodiumimmunitybyalitafliketranscriptionfactorinthemalariavectoranophelesgambiae AT abrahamgeappen regulationofantiplasmodiumimmunitybyalitafliketranscriptionfactorinthemalariavectoranophelesgambiae AT andreajradtke regulationofantiplasmodiumimmunitybyalitafliketranscriptionfactorinthemalariavectoranophelesgambiae AT marcelojacobslorena regulationofantiplasmodiumimmunitybyalitafliketranscriptionfactorinthemalariavectoranophelesgambiae
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Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.

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