Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy

Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and demonstrate high throughput FLIM on standard camera se...

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Main Authors:	Adam J. Bowman, Brannon B. Klopfer, Thomas Juffmann, Mark A. Kasevich
Format:	article
Language:	EN
Published:	Nature Portfolio 2019
Subjects:	Science Q
Online Access:	https://doaj.org/article/7476a65533a64efdaa7ac34d856725f6
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id	oai:doaj.org-article:7476a65533a64efdaa7ac34d856725f6
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spelling	oai:doaj.org-article:7476a65533a64efdaa7ac34d856725f62021-12-02T15:36:23ZElectro-optic imaging enables efficient wide-field fluorescence lifetime microscopy10.1038/s41467-019-12535-52041-1723https://doaj.org/article/7476a65533a64efdaa7ac34d856725f62019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12535-5https://doaj.org/toc/2041-1723Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and demonstrate high throughput FLIM on standard camera sensors.Adam J. BowmanBrannon B. KlopferThomas JuffmannMark A. KasevichNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Adam J. Bowman Brannon B. Klopfer Thomas Juffmann Mark A. Kasevich Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
description	Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and demonstrate high throughput FLIM on standard camera sensors.
format	article
author	Adam J. Bowman Brannon B. Klopfer Thomas Juffmann Mark A. Kasevich
author_facet	Adam J. Bowman Brannon B. Klopfer Thomas Juffmann Mark A. Kasevich
author_sort	Adam J. Bowman
title	Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_short	Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_full	Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_fullStr	Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_full_unstemmed	Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_sort	electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7476a65533a64efdaa7ac34d856725f6
work_keys_str_mv	AT adamjbowman electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy AT brannonbklopfer electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy AT thomasjuffmann electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy AT markakasevich electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy
_version_	1718386296405098496

Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy

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