Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis

Chemical tools to monitor drug-target engagement of endogenous enzymes are essential for preclinical target validation. Here, the authors present a chemical genetics strategy to study target engagement of endogenous kinases, achieving specific labeling and inactivation of FES kinase to provide insig...

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Autores principales:	Tom van der Wel, Riet Hilhorst, Hans den Dulk, Tim van den Hooven, Nienke M. Prins, Joost A. P. M. Wijnakker, Bogdan I. Florea, Eelke B. Lenselink, Gerard J. P. van Westen, Rob Ruijtenbeek, Herman S. Overkleeft, Allard Kaptein, Tjeerd Barf, Mario van der Stelt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/749ac4b664044b4d9100599885c8e063
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spelling	oai:doaj.org-article:749ac4b664044b4d9100599885c8e0632021-12-02T17:45:15ZChemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis10.1038/s41467-020-17027-52041-1723https://doaj.org/article/749ac4b664044b4d9100599885c8e0632020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17027-5https://doaj.org/toc/2041-1723Chemical tools to monitor drug-target engagement of endogenous enzymes are essential for preclinical target validation. Here, the authors present a chemical genetics strategy to study target engagement of endogenous kinases, achieving specific labeling and inactivation of FES kinase to provide insights into FES’ role in neutrophil phagocytosis.Tom van der WelRiet HilhorstHans den DulkTim van den HoovenNienke M. PrinsJoost A. P. M. WijnakkerBogdan I. FloreaEelke B. LenselinkGerard J. P. van WestenRob RuijtenbeekHerman S. OverkleeftAllard KapteinTjeerd BarfMario van der SteltNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-20 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tom van der Wel Riet Hilhorst Hans den Dulk Tim van den Hooven Nienke M. Prins Joost A. P. M. Wijnakker Bogdan I. Florea Eelke B. Lenselink Gerard J. P. van Westen Rob Ruijtenbeek Herman S. Overkleeft Allard Kaptein Tjeerd Barf Mario van der Stelt Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
description	Chemical tools to monitor drug-target engagement of endogenous enzymes are essential for preclinical target validation. Here, the authors present a chemical genetics strategy to study target engagement of endogenous kinases, achieving specific labeling and inactivation of FES kinase to provide insights into FES’ role in neutrophil phagocytosis.
format	article
author	Tom van der Wel Riet Hilhorst Hans den Dulk Tim van den Hooven Nienke M. Prins Joost A. P. M. Wijnakker Bogdan I. Florea Eelke B. Lenselink Gerard J. P. van Westen Rob Ruijtenbeek Herman S. Overkleeft Allard Kaptein Tjeerd Barf Mario van der Stelt
author_facet	Tom van der Wel Riet Hilhorst Hans den Dulk Tim van den Hooven Nienke M. Prins Joost A. P. M. Wijnakker Bogdan I. Florea Eelke B. Lenselink Gerard J. P. van Westen Rob Ruijtenbeek Herman S. Overkleeft Allard Kaptein Tjeerd Barf Mario van der Stelt
author_sort	Tom van der Wel
title	Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
title_short	Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
title_full	Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
title_fullStr	Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
title_full_unstemmed	Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
title_sort	chemical genetics strategy to profile kinase target engagement reveals role of fes in neutrophil phagocytosis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/749ac4b664044b4d9100599885c8e063
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Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis

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