Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate

ABSTRACT Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli. Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the under...

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Autores principales:	Jingyu Diao, Catrien Bouwman, Donghong Yan, Jing Kang, Anand K. Katakam, Peter Liu, Homer Pantua, Alexander R. Abbas, Nicholas N. Nickerson, Cary Austin, Mike Reichelt, Wendy Sandoval, Min Xu, Chris Whitfield, Sharookh B. Kapadia
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Escherichia coli capsular polysaccharide cell envelope complement resistance murein lipoprotein polysaccharide export Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/749b1c49d18e422d93c20f1e9940a9d9
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spelling	oai:doaj.org-article:749b1c49d18e422d93c20f1e9940a9d92021-11-15T15:51:30ZPeptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate10.1128/mBio.00603-172150-7511https://doaj.org/article/749b1c49d18e422d93c20f1e9940a9d92017-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00603-17https://doaj.org/toc/2150-7511ABSTRACT Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli. Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the underlying mechanism has not been established. Here, lpp and pal mutants of uropathogenic E. coli strain CFT073 showed reduced survival in a mouse bacteremia model, but only the lpp mutant was sensitive to serum killing in vitro. The peptidoglycan-bound Lpp form was specifically required for preventing complement-mediated bacterial lysis in vitro and complement-mediated clearance in vivo. Compared to the wild-type strain, the lpp mutant had impaired K2 capsular polysaccharide production and was unable to respond to exposure to serum by elevating capsular polysaccharide amounts. These properties correlated with altered cellular distribution of KpsD, the predicted outer membrane translocon for “group 2” capsular polysaccharides. We identified a novel Lpp-dependent association between functional KpsD and peptidoglycan, highlighting important interplay between cell envelope components required for resistance to complement-mediated lysis in uropathogenic E. coli isolates. IMPORTANCE Uropathogenic E. coli (UPEC) isolates represent a significant cause of nosocomial urinary tract and bloodstream infections. Many UPEC isolates are resistant to serum killing. Here, we show that a major cell-envelope lipoprotein (murein lipoprotein) is required for serum resistance in vitro and for complement-mediated bacterial clearance in vivo. This is mediated, in part, through a novel mechanism by which murein lipoprotein affects the proper assembly of a key component of the machinery involved in production of “group 2” capsules. The absence of murein lipoprotein results in impaired production of the capsule layer, a known participant in complement resistance. These results demonstrate an important role for murein lipoprotein in complex interactions between different outer membrane biogenesis pathways and further highlight the importance of lipoprotein assembly and transport in bacterial pathogenesis.Jingyu DiaoCatrien BouwmanDonghong YanJing KangAnand K. KatakamPeter LiuHomer PantuaAlexander R. AbbasNicholas N. NickersonCary AustinMike ReicheltWendy SandovalMin XuChris WhitfieldSharookh B. KapadiaAmerican Society for MicrobiologyarticleEscherichia colicapsular polysaccharidecell envelopecomplement resistancemurein lipoproteinpolysaccharide exportMicrobiologyQR1-502ENmBio, Vol 8, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli capsular polysaccharide cell envelope complement resistance murein lipoprotein polysaccharide export Microbiology QR1-502
spellingShingle	Escherichia coli capsular polysaccharide cell envelope complement resistance murein lipoprotein polysaccharide export Microbiology QR1-502 Jingyu Diao Catrien Bouwman Donghong Yan Jing Kang Anand K. Katakam Peter Liu Homer Pantua Alexander R. Abbas Nicholas N. Nickerson Cary Austin Mike Reichelt Wendy Sandoval Min Xu Chris Whitfield Sharookh B. Kapadia Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate
description	ABSTRACT Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli. Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the underlying mechanism has not been established. Here, lpp and pal mutants of uropathogenic E. coli strain CFT073 showed reduced survival in a mouse bacteremia model, but only the lpp mutant was sensitive to serum killing in vitro. The peptidoglycan-bound Lpp form was specifically required for preventing complement-mediated bacterial lysis in vitro and complement-mediated clearance in vivo. Compared to the wild-type strain, the lpp mutant had impaired K2 capsular polysaccharide production and was unable to respond to exposure to serum by elevating capsular polysaccharide amounts. These properties correlated with altered cellular distribution of KpsD, the predicted outer membrane translocon for “group 2” capsular polysaccharides. We identified a novel Lpp-dependent association between functional KpsD and peptidoglycan, highlighting important interplay between cell envelope components required for resistance to complement-mediated lysis in uropathogenic E. coli isolates. IMPORTANCE Uropathogenic E. coli (UPEC) isolates represent a significant cause of nosocomial urinary tract and bloodstream infections. Many UPEC isolates are resistant to serum killing. Here, we show that a major cell-envelope lipoprotein (murein lipoprotein) is required for serum resistance in vitro and for complement-mediated bacterial clearance in vivo. This is mediated, in part, through a novel mechanism by which murein lipoprotein affects the proper assembly of a key component of the machinery involved in production of “group 2” capsules. The absence of murein lipoprotein results in impaired production of the capsule layer, a known participant in complement resistance. These results demonstrate an important role for murein lipoprotein in complex interactions between different outer membrane biogenesis pathways and further highlight the importance of lipoprotein assembly and transport in bacterial pathogenesis.
format	article
author	Jingyu Diao Catrien Bouwman Donghong Yan Jing Kang Anand K. Katakam Peter Liu Homer Pantua Alexander R. Abbas Nicholas N. Nickerson Cary Austin Mike Reichelt Wendy Sandoval Min Xu Chris Whitfield Sharookh B. Kapadia
author_facet	Jingyu Diao Catrien Bouwman Donghong Yan Jing Kang Anand K. Katakam Peter Liu Homer Pantua Alexander R. Abbas Nicholas N. Nickerson Cary Austin Mike Reichelt Wendy Sandoval Min Xu Chris Whitfield Sharookh B. Kapadia
author_sort	Jingyu Diao
title	Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate
title_short	Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate
title_full	Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate
title_fullStr	Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate
title_full_unstemmed	Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate
title_sort	peptidoglycan association of murein lipoprotein is required for kpsd-dependent group 2 capsular polysaccharide expression and serum resistance in a uropathogenic <italic toggle="yes">escherichia coli</italic> isolate
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/749b1c49d18e422d93c20f1e9940a9d9
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Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic <italic toggle="yes">Escherichia coli</italic> Isolate

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