Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model

Rahul Pathak,1 Steve F Bierman,2 Pieter d’Arnaud3 1University of Central Florida College of Medicine, Lakeland, FL 33813, USA; 2Access Scientific, LLC, San Diego, CA 92121, USA; 3Instat Consulting, Inc., Chatham, NJ 07928, USA Introduction: Despite sterile barrier precautions and...

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Autores principales:	Pathak R, Bierman SF, d'Arnaud P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Biofilm CRBSI CLABSI catheter-related infections catheter-associated infections bacterial resistance Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/749cb8f7022d45a88d4e066100d20fc7
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spelling	oai:doaj.org-article:749cb8f7022d45a88d4e066100d20fc72021-12-02T04:37:47ZInhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model1179-1470https://doaj.org/article/749cb8f7022d45a88d4e066100d20fc72018-12-01T00:00:00Zhttps://www.dovepress.com/inhibition-of-bacterial-attachment-and-biofilm-formation-by-a-novel-in-peer-reviewed-article-MDERhttps://doaj.org/toc/1179-1470Rahul Pathak,1 Steve F Bierman,2 Pieter d’Arnaud3 1University of Central Florida College of Medicine, Lakeland, FL 33813, USA; 2Access Scientific, LLC, San Diego, CA 92121, USA; 3Instat Consulting, Inc., Chatham, NJ 07928, USA Introduction: Despite sterile barrier precautions and vigorous skin antisepsis, percutaneous insertion of intravenous catheters has been shown to result in attachment to the catheter surface of bacteria residing in the deep structures of the skin. Such attachment poses the risk of biofilm formation and eventual catheter-related bloodstream infection (CRBSI). This study was undertaken to assess whether the non-coated surface treatment of a unique catheter material (ChronoFlex C® with BioGUARD™) could inhibit bacterial attachment and biofilm formation.Methods: A novel in vitro model and fluorescence microscopy were used to compare two intravascular catheter materials with respect to bacterial attachment and biofilm formation. The control material was a commonly used polyurethane. The study material was a unique copolymer, treated so as to remove surface additives, alter hydrophobicity and create surface micro-patterning. Outcomes were assessed using both a membrane potential indicator and a cell death reporter with appropriate fluorescent channels. Thus, bacterial cells attached to the catheter surface (living and dead) were imaged without mechanical disruption.Results: Both bacterial attachment and biofilm formation are significantly inhibited by the study catheter material. In fact, over 5 times more bacteria were able to attach and grow on the control polyurethane material than on the study material (P=0.0020). Moreover, those few bacteria that were able to attach to the study material had a 1.5 times greater likelihood of dying. Conclusion: Using a novel in vitro percutaneous catheter insertion model, ChronoFlex C with BioGUARD is proven to significantly inhibit bacterial attachment and biofilm formation as compared with a commonly used polyurethane catheter material. Keywords: biofilm, CRBSI, CLABSI, catheter-related infections, catheter-associated infections, bacterial resistancePathak RBierman SFd'Arnaud PDove Medical PressarticleBiofilmCRBSICLABSIcatheter-related infectionscatheter-associated infectionsbacterial resistanceMedical technologyR855-855.5ENMedical Devices: Evidence and Research, Vol Volume 11, Pp 427-432 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biofilm CRBSI CLABSI catheter-related infections catheter-associated infections bacterial resistance Medical technology R855-855.5
spellingShingle	Biofilm CRBSI CLABSI catheter-related infections catheter-associated infections bacterial resistance Medical technology R855-855.5 Pathak R Bierman SF d'Arnaud P Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
description	Rahul Pathak,1 Steve F Bierman,2 Pieter d’Arnaud3 1University of Central Florida College of Medicine, Lakeland, FL 33813, USA; 2Access Scientific, LLC, San Diego, CA 92121, USA; 3Instat Consulting, Inc., Chatham, NJ 07928, USA Introduction: Despite sterile barrier precautions and vigorous skin antisepsis, percutaneous insertion of intravenous catheters has been shown to result in attachment to the catheter surface of bacteria residing in the deep structures of the skin. Such attachment poses the risk of biofilm formation and eventual catheter-related bloodstream infection (CRBSI). This study was undertaken to assess whether the non-coated surface treatment of a unique catheter material (ChronoFlex C® with BioGUARD™) could inhibit bacterial attachment and biofilm formation.Methods: A novel in vitro model and fluorescence microscopy were used to compare two intravascular catheter materials with respect to bacterial attachment and biofilm formation. The control material was a commonly used polyurethane. The study material was a unique copolymer, treated so as to remove surface additives, alter hydrophobicity and create surface micro-patterning. Outcomes were assessed using both a membrane potential indicator and a cell death reporter with appropriate fluorescent channels. Thus, bacterial cells attached to the catheter surface (living and dead) were imaged without mechanical disruption.Results: Both bacterial attachment and biofilm formation are significantly inhibited by the study catheter material. In fact, over 5 times more bacteria were able to attach and grow on the control polyurethane material than on the study material (P=0.0020). Moreover, those few bacteria that were able to attach to the study material had a 1.5 times greater likelihood of dying. Conclusion: Using a novel in vitro percutaneous catheter insertion model, ChronoFlex C with BioGUARD is proven to significantly inhibit bacterial attachment and biofilm formation as compared with a commonly used polyurethane catheter material. Keywords: biofilm, CRBSI, CLABSI, catheter-related infections, catheter-associated infections, bacterial resistance
format	article
author	Pathak R Bierman SF d'Arnaud P
author_facet	Pathak R Bierman SF d'Arnaud P
author_sort	Pathak R
title	Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
title_short	Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
title_full	Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
title_fullStr	Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
title_full_unstemmed	Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
title_sort	inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/749cb8f7022d45a88d4e066100d20fc7
work_keys_str_mv	AT pathakr inhibitionofbacterialattachmentandbiofilmformationbyanovelintravenouscathetermaterialusinganinvitropercutaneouscatheterinsertionmodel AT biermansf inhibitionofbacterialattachmentandbiofilmformationbyanovelintravenouscathetermaterialusinganinvitropercutaneouscatheterinsertionmodel AT darnaudp inhibitionofbacterialattachmentandbiofilmformationbyanovelintravenouscathetermaterialusinganinvitropercutaneouscatheterinsertionmodel
_version_	1718401127296270336

Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model

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