Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy

Background: Schistosomiasis is a parasitic disease caused by hematodes of genus Schistosoma. This review evaluated the available nucleic acid amplification techniques for diagnosing S. mansoni infections in humans, intermediate host snails, and presumed rodent reservoirs. Methods: Sensitivity, speci...

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Autores principales:	Hong-Mei Li, Zhi-Qiang Qin, Robert Bergquist, Men-Bao Qian, Shang Xia, Shan Lv, Ning Xiao, Jurg Utzinger, Xiao-Nong Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Schistosomiasis Schistosoma mansoni Human diagnosis Snail diagnosis Nucleic acid amplification technique PCR-ELISA Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/749df8596e924afe9fd7fcc8dd3c2d8f
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spelling	oai:doaj.org-article:749df8596e924afe9fd7fcc8dd3c2d8f2021-11-30T04:14:02ZNucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy1201-971210.1016/j.ijid.2021.08.061https://doaj.org/article/749df8596e924afe9fd7fcc8dd3c2d8f2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1201971221006949https://doaj.org/toc/1201-9712Background: Schistosomiasis is a parasitic disease caused by hematodes of genus Schistosoma. This review evaluated the available nucleic acid amplification techniques for diagnosing S. mansoni infections in humans, intermediate host snails, and presumed rodent reservoirs. Methods: Sensitivity, specificity, diagnostic odds ratio (DOR), and 95% CI were calculated based on available literature. The potential of PCR, nPCR, PCR-ELISA, qPCR, and LAMP was compared for diagnosing S. mansoni infections. Results: A total of 546 published records were identified. Quality assessment by QUADAS-2 revealed an uncertain risk in most studies, and 21 references were included in the final. For human samples, the four nucleic acid amplification techniques showed an overall sensitivity of 89.79% (95% CI: 83.92%-93.67%), specificity of 87.70% (95% CI: 72.60%-95.05%), and DOR of 37.73 (95% CI: 21.79-65.33). LAMP showed the highest sensitivity, followed by PCR-ELISA, PCR, and qPCR, while this order was almost reversed for specificity; qPCR had the highest AUC. For rodent samples, qPCR showed modest sensitivity (68.75%, 95% CI: 43.32%-86.36%) and high specificity (92.45%, 95% CI: 19.94%-99.83%). For snail samples, PCR and nPCR assays showed high sensitivity of 90.06% (95% CI: 84.39%-93.82%) and specificity of 85.51% (95% CI: 54.39%-96.69%). Conclusion: Nucleic acid amplification techniques had high diagnostic potential for identifying S. mansoni infections in humans.Hong-Mei LiZhi-Qiang QinRobert BergquistMen-Bao QianShang XiaShan LvNing XiaoJurg UtzingerXiao-Nong ZhouElsevierarticleSchistosomiasisSchistosoma mansoniHuman diagnosisSnail diagnosisNucleic acid amplification techniquePCR-ELISAInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 112, Iss , Pp 152-164 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Schistosomiasis Schistosoma mansoni Human diagnosis Snail diagnosis Nucleic acid amplification technique PCR-ELISA Infectious and parasitic diseases RC109-216
spellingShingle	Schistosomiasis Schistosoma mansoni Human diagnosis Snail diagnosis Nucleic acid amplification technique PCR-ELISA Infectious and parasitic diseases RC109-216 Hong-Mei Li Zhi-Qiang Qin Robert Bergquist Men-Bao Qian Shang Xia Shan Lv Ning Xiao Jurg Utzinger Xiao-Nong Zhou Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
description	Background: Schistosomiasis is a parasitic disease caused by hematodes of genus Schistosoma. This review evaluated the available nucleic acid amplification techniques for diagnosing S. mansoni infections in humans, intermediate host snails, and presumed rodent reservoirs. Methods: Sensitivity, specificity, diagnostic odds ratio (DOR), and 95% CI were calculated based on available literature. The potential of PCR, nPCR, PCR-ELISA, qPCR, and LAMP was compared for diagnosing S. mansoni infections. Results: A total of 546 published records were identified. Quality assessment by QUADAS-2 revealed an uncertain risk in most studies, and 21 references were included in the final. For human samples, the four nucleic acid amplification techniques showed an overall sensitivity of 89.79% (95% CI: 83.92%-93.67%), specificity of 87.70% (95% CI: 72.60%-95.05%), and DOR of 37.73 (95% CI: 21.79-65.33). LAMP showed the highest sensitivity, followed by PCR-ELISA, PCR, and qPCR, while this order was almost reversed for specificity; qPCR had the highest AUC. For rodent samples, qPCR showed modest sensitivity (68.75%, 95% CI: 43.32%-86.36%) and high specificity (92.45%, 95% CI: 19.94%-99.83%). For snail samples, PCR and nPCR assays showed high sensitivity of 90.06% (95% CI: 84.39%-93.82%) and specificity of 85.51% (95% CI: 54.39%-96.69%). Conclusion: Nucleic acid amplification techniques had high diagnostic potential for identifying S. mansoni infections in humans.
format	article
author	Hong-Mei Li Zhi-Qiang Qin Robert Bergquist Men-Bao Qian Shang Xia Shan Lv Ning Xiao Jurg Utzinger Xiao-Nong Zhou
author_facet	Hong-Mei Li Zhi-Qiang Qin Robert Bergquist Men-Bao Qian Shang Xia Shan Lv Ning Xiao Jurg Utzinger Xiao-Nong Zhou
author_sort	Hong-Mei Li
title	Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
title_short	Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
title_full	Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
title_fullStr	Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
title_full_unstemmed	Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
title_sort	nucleic acid amplification techniques for the detection of schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/749df8596e924afe9fd7fcc8dd3c2d8f
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Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy

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