Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy

Quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing e...

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Autores principales:	Hung-Shih Lin, Robert Kuo-Kuang Lee, Tsung-Hsien Yang, Hsu-Wei Fang, Sheng-Hsiang Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	quiescin sulfhydryl oxidase 1 uterus placenta embryo maternal-fetal interface tissue distribution Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/749e8c6f185647bbb513466b04ab8eb1
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spelling	oai:doaj.org-article:749e8c6f185647bbb513466b04ab8eb12021-11-11T15:13:04ZSpatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy10.3390/app1121101512076-3417https://doaj.org/article/749e8c6f185647bbb513466b04ab8eb12021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10151https://doaj.org/toc/2076-3417Quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing embryo during mouse pregnancy. Immunohistochemical staining was used to reveal the localization of QSOX1 protein, and HistoQuest was applied to quantify protein levels. The expression level of QSOX1 in the decidua and muscle cells of the pregnant uterus fluctuated dramatically during pregnancy. QSOX1 was ubiquitously expressed in the labyrinth, junction zone, and chorionic plate in the placenta. The quantitative analysis found that this protein was highly expressed in the spinal cord, lens, midbrain, cerebellum, medulla oblongata, and tooth of mouse embryos, followed by the heart, intercostal muscle, diaphragm, intermediate zone, extrinsic ocular muscle, spine, pons, epidermis, tongue, ganglion, vomeronasal organ, thoracic vertebrae, and thymus. Interestingly, QSOX1 was also markedly expressed in olfactory system tissues. This comprehensive spatiotemporal study of QSOX1 protein expression will provide a basis for further investigations of the QSOX1 physiological function in the pregnant uterus, placenta, and developing embryo.Hung-Shih LinRobert Kuo-Kuang LeeTsung-Hsien YangHsu-Wei FangSheng-Hsiang LiMDPI AGarticlequiescin sulfhydryl oxidase 1uterusplacentaembryomaternal-fetal interfacetissue distributionTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10151, p 10151 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quiescin sulfhydryl oxidase 1 uterus placenta embryo maternal-fetal interface tissue distribution Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	quiescin sulfhydryl oxidase 1 uterus placenta embryo maternal-fetal interface tissue distribution Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Hung-Shih Lin Robert Kuo-Kuang Lee Tsung-Hsien Yang Hsu-Wei Fang Sheng-Hsiang Li Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
description	Quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing embryo during mouse pregnancy. Immunohistochemical staining was used to reveal the localization of QSOX1 protein, and HistoQuest was applied to quantify protein levels. The expression level of QSOX1 in the decidua and muscle cells of the pregnant uterus fluctuated dramatically during pregnancy. QSOX1 was ubiquitously expressed in the labyrinth, junction zone, and chorionic plate in the placenta. The quantitative analysis found that this protein was highly expressed in the spinal cord, lens, midbrain, cerebellum, medulla oblongata, and tooth of mouse embryos, followed by the heart, intercostal muscle, diaphragm, intermediate zone, extrinsic ocular muscle, spine, pons, epidermis, tongue, ganglion, vomeronasal organ, thoracic vertebrae, and thymus. Interestingly, QSOX1 was also markedly expressed in olfactory system tissues. This comprehensive spatiotemporal study of QSOX1 protein expression will provide a basis for further investigations of the QSOX1 physiological function in the pregnant uterus, placenta, and developing embryo.
format	article
author	Hung-Shih Lin Robert Kuo-Kuang Lee Tsung-Hsien Yang Hsu-Wei Fang Sheng-Hsiang Li
author_facet	Hung-Shih Lin Robert Kuo-Kuang Lee Tsung-Hsien Yang Hsu-Wei Fang Sheng-Hsiang Li
author_sort	Hung-Shih Lin
title	Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
title_short	Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
title_full	Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
title_fullStr	Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
title_full_unstemmed	Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
title_sort	spatiotemporal protein expression profiles of qsox1 in the murine uterus, placenta, and embryo during pregnancy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/749e8c6f185647bbb513466b04ab8eb1
work_keys_str_mv	AT hungshihlin spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT robertkuokuanglee spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT tsunghsienyang spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT hsuweifang spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT shenghsiangli spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy
_version_	1718436660070318080

Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy

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