Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer

Michal Kolitz-Domb, Enav Corem-Salkmon, Igor Grinberg, Shlomo Margel Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat Gan, Israel Abstract: Colon cancer is one of the major causes of death in the Western world. Early detection significantly i...

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Autores principales:	Kolitz-Domb M, Corem-Salkmon E, Grinberg I, Margel S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/74a2600eac4d41adb31391adca4aa045
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spelling	oai:doaj.org-article:74a2600eac4d41adb31391adca4aa0452021-12-02T07:13:44ZSynthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer1178-2013https://doaj.org/article/74a2600eac4d41adb31391adca4aa0452014-10-01T00:00:00Zhttp://www.dovepress.com/synthesis-and-characterization-of-bioactive-conjugated-near-infrared-f-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Michal Kolitz-Domb, Enav Corem-Salkmon, Igor Grinberg, Shlomo Margel Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat Gan, Israel Abstract: Colon cancer is one of the major causes of death in the Western world. Early detection significantly improves long-term survival for patients with colon cancer. Near-infrared (NIR) fluorescent nanoparticles are promising candidates for use as contrast agents for tumor detection. Using NIR offers several advantages for bioimaging compared with fluorescence in the visible spectrum: lower autofluorescence of biological tissues and lower absorbance and, consequently, deeper penetration into biomatrices. The present study describes the preparation of new NIR fluorescent proteinoid-poly(L-lactic acid) (PLLA) nanoparticles. For this purpose, a P(EF-PLLA) random copolymer was prepared by thermal copolymerization of L-glutamic acid (E) with L-phenylalanine (F) and PLLA. Under suitable conditions, this proteinoid-PLLA copolymer can self-assemble to nanosized hollow particles of relatively narrow size distribution. This self-assembly process was used for encapsulation of the NIR dye indocyanine green. The encapsulation process increases significantly the photostability of the dye. These NIR fluorescent nanoparticles were found to be stable and nontoxic. Leakage of the NIR dye from these nanoparticles into phosphate-buffered saline containing 4% human serum albumin was not detected. Tumor-targeting ligands such as peanut agglutinin and anticarcinoembryonic antigen antibodies were covalently conjugated to the surface of the NIR fluorescent P(EF-PLLA) nanoparticles, thereby increasing the fluorescent signal of tumors with upregulated corresponding receptors. Specific colon tumor detection by the NIR fluorescent P(EF-PLLA) nanoparticles was demonstrated in a chicken embryo model. In future work, we plan to extend this study to a mouse model, as well as to encapsulate a cancer drug such as doxorubicin within these nanoparticles for therapeutic applications. Keywords: proteinoid, self-assembly, thermal condensation, hollow nanoparticles, fluorescent nanoparticles, NIR fluorescenceKolitz-Domb MCorem-Salkmon EGrinberg IMargel SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5041-5053 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kolitz-Domb M Corem-Salkmon E Grinberg I Margel S Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer
description	Michal Kolitz-Domb, Enav Corem-Salkmon, Igor Grinberg, Shlomo Margel Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat Gan, Israel Abstract: Colon cancer is one of the major causes of death in the Western world. Early detection significantly improves long-term survival for patients with colon cancer. Near-infrared (NIR) fluorescent nanoparticles are promising candidates for use as contrast agents for tumor detection. Using NIR offers several advantages for bioimaging compared with fluorescence in the visible spectrum: lower autofluorescence of biological tissues and lower absorbance and, consequently, deeper penetration into biomatrices. The present study describes the preparation of new NIR fluorescent proteinoid-poly(L-lactic acid) (PLLA) nanoparticles. For this purpose, a P(EF-PLLA) random copolymer was prepared by thermal copolymerization of L-glutamic acid (E) with L-phenylalanine (F) and PLLA. Under suitable conditions, this proteinoid-PLLA copolymer can self-assemble to nanosized hollow particles of relatively narrow size distribution. This self-assembly process was used for encapsulation of the NIR dye indocyanine green. The encapsulation process increases significantly the photostability of the dye. These NIR fluorescent nanoparticles were found to be stable and nontoxic. Leakage of the NIR dye from these nanoparticles into phosphate-buffered saline containing 4% human serum albumin was not detected. Tumor-targeting ligands such as peanut agglutinin and anticarcinoembryonic antigen antibodies were covalently conjugated to the surface of the NIR fluorescent P(EF-PLLA) nanoparticles, thereby increasing the fluorescent signal of tumors with upregulated corresponding receptors. Specific colon tumor detection by the NIR fluorescent P(EF-PLLA) nanoparticles was demonstrated in a chicken embryo model. In future work, we plan to extend this study to a mouse model, as well as to encapsulate a cancer drug such as doxorubicin within these nanoparticles for therapeutic applications. Keywords: proteinoid, self-assembly, thermal condensation, hollow nanoparticles, fluorescent nanoparticles, NIR fluorescence
format	article
author	Kolitz-Domb M Corem-Salkmon E Grinberg I Margel S
author_facet	Kolitz-Domb M Corem-Salkmon E Grinberg I Margel S
author_sort	Kolitz-Domb M
title	Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer
title_short	Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer
title_full	Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer
title_fullStr	Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer
title_full_unstemmed	Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer
title_sort	synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(l-lactic acid) hollow nanoparticles for optical detection of colon cancer
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/74a2600eac4d41adb31391adca4aa045
work_keys_str_mv	AT kolitzdombm synthesisandcharacterizationofbioactiveconjugatednearinfraredfluorescentproteinoidpolyllacticacidhollownanoparticlesforopticaldetectionofcoloncancer AT coremsalkmone synthesisandcharacterizationofbioactiveconjugatednearinfraredfluorescentproteinoidpolyllacticacidhollownanoparticlesforopticaldetectionofcoloncancer AT grinbergi synthesisandcharacterizationofbioactiveconjugatednearinfraredfluorescentproteinoidpolyllacticacidhollownanoparticlesforopticaldetectionofcoloncancer AT margels synthesisandcharacterizationofbioactiveconjugatednearinfraredfluorescentproteinoidpolyllacticacidhollownanoparticlesforopticaldetectionofcoloncancer
_version_	1718399521893908480

Synthesis and characterization of bioactive conjugated near-infrared fluorescent proteinoid-poly(L-lactic acid) hollow nanoparticles for optical detection of colon cancer

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