A comprehensive analysis and annotation of human normal urinary proteome

Abstract Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of...

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Autores principales:	Mindi Zhao, Menglin Li, Yehong Yang, Zhengguang Guo, Ying Sun, Chen Shao, Mingxi Li, Wei Sun, Youhe Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/74acc12c19d04656853d3c4688c0cff8
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spelling	oai:doaj.org-article:74acc12c19d04656853d3c4688c0cff82021-12-02T11:53:00ZA comprehensive analysis and annotation of human normal urinary proteome10.1038/s41598-017-03226-62045-2322https://doaj.org/article/74acc12c19d04656853d3c4688c0cff82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03226-6https://doaj.org/toc/2045-2322Abstract Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography–tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS. A total of 6085 proteins were identified in healthy urine, of which 2001 were not reported in previous studies and the concentrations of 2571 proteins were estimated (spanning a magnitude of 106) with an intensity-based absolute quantification algorithm. The urinary proteins were annotated by their tissue distribution. Detailed information can be accessed at the “Human Urine Proteome Database” (www.urimarker.com/urine).Mindi ZhaoMenglin LiYehong YangZhengguang GuoYing SunChen ShaoMingxi LiWei SunYouhe GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mindi Zhao Menglin Li Yehong Yang Zhengguang Guo Ying Sun Chen Shao Mingxi Li Wei Sun Youhe Gao A comprehensive analysis and annotation of human normal urinary proteome
description	Abstract Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography–tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS. A total of 6085 proteins were identified in healthy urine, of which 2001 were not reported in previous studies and the concentrations of 2571 proteins were estimated (spanning a magnitude of 106) with an intensity-based absolute quantification algorithm. The urinary proteins were annotated by their tissue distribution. Detailed information can be accessed at the “Human Urine Proteome Database” (www.urimarker.com/urine).
format	article
author	Mindi Zhao Menglin Li Yehong Yang Zhengguang Guo Ying Sun Chen Shao Mingxi Li Wei Sun Youhe Gao
author_facet	Mindi Zhao Menglin Li Yehong Yang Zhengguang Guo Ying Sun Chen Shao Mingxi Li Wei Sun Youhe Gao
author_sort	Mindi Zhao
title	A comprehensive analysis and annotation of human normal urinary proteome
title_short	A comprehensive analysis and annotation of human normal urinary proteome
title_full	A comprehensive analysis and annotation of human normal urinary proteome
title_fullStr	A comprehensive analysis and annotation of human normal urinary proteome
title_full_unstemmed	A comprehensive analysis and annotation of human normal urinary proteome
title_sort	comprehensive analysis and annotation of human normal urinary proteome
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/74acc12c19d04656853d3c4688c0cff8
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A comprehensive analysis and annotation of human normal urinary proteome

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