Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance

ABSTRACT The basal body shares similar architecture with centrioles in animals and is involved in nucleating flagellar axonemal microtubules in flagellated eukaryotes. The early-branching Trypanosoma brucei possesses a motile flagellum nucleated from the basal body that consists of a mature basal bo...

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Autores principales:	Hung Quang Dang, Qing Zhou, Veronica W. Rowlett, Huiqing Hu, Kyu Joon Lee, William Margolin, Ziyin Li
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/74af67cc051d4202bd3f0164ad2c2d68
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spelling	oai:doaj.org-article:74af67cc051d4202bd3f0164ad2c2d682021-11-15T15:51:07ZProximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance10.1128/mBio.02120-162150-7511https://doaj.org/article/74af67cc051d4202bd3f0164ad2c2d682017-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02120-16https://doaj.org/toc/2150-7511ABSTRACT The basal body shares similar architecture with centrioles in animals and is involved in nucleating flagellar axonemal microtubules in flagellated eukaryotes. The early-branching Trypanosoma brucei possesses a motile flagellum nucleated from the basal body that consists of a mature basal body and an adjacent pro-basal body. Little is known about the basal body proteome and its roles in basal body biogenesis and flagellar axoneme assembly in T. brucei. Here, we report the identification of 14 conserved centriole/basal body protein homologs and 25 trypanosome-specific basal body proteins. These proteins localize to distinct subdomains of the basal body, and several of them form a ring-like structure surrounding the basal body barrel. Functional characterization of representative basal body proteins revealed distinct roles in basal body duplication/separation and flagellar axoneme assembly. Overall, this work identified novel proteins required for basal body duplication and separation and uncovered new functions of conserved basal body proteins in basal body duplication and separation, highlighting an unusual mechanism of basal body biogenesis and inheritance in this early divergent eukaryote. IMPORTANCE The basal body in the early-branching protozoan Trypanosoma brucei nucleates flagellum assembly and also regulates organelle segregation, cell morphogenesis, and cell division. However, the molecular composition and the assembly process of the basal body remain poorly understood. Here, we identify 14 conserved basal body proteins and 25 trypanosome-specific basal body proteins via bioinformatics, localization-based screening, and proximity-dependent biotin identification. We further localized these proteins to distinct subdomains of the basal body by using fluorescence microscopy and superresolution microscopy, discovered novel regulators of basal body duplication and separation, and uncovered new functions of conserved basal body proteins in basal body duplication and separation. This work lays the foundation for dissecting the mechanisms underlying basal body biogenesis and inheritance in T. brucei.Hung Quang DangQing ZhouVeronica W. RowlettHuiqing HuKyu Joon LeeWilliam MargolinZiyin LiAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 8, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Hung Quang Dang Qing Zhou Veronica W. Rowlett Huiqing Hu Kyu Joon Lee William Margolin Ziyin Li Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance
description	ABSTRACT The basal body shares similar architecture with centrioles in animals and is involved in nucleating flagellar axonemal microtubules in flagellated eukaryotes. The early-branching Trypanosoma brucei possesses a motile flagellum nucleated from the basal body that consists of a mature basal body and an adjacent pro-basal body. Little is known about the basal body proteome and its roles in basal body biogenesis and flagellar axoneme assembly in T. brucei. Here, we report the identification of 14 conserved centriole/basal body protein homologs and 25 trypanosome-specific basal body proteins. These proteins localize to distinct subdomains of the basal body, and several of them form a ring-like structure surrounding the basal body barrel. Functional characterization of representative basal body proteins revealed distinct roles in basal body duplication/separation and flagellar axoneme assembly. Overall, this work identified novel proteins required for basal body duplication and separation and uncovered new functions of conserved basal body proteins in basal body duplication and separation, highlighting an unusual mechanism of basal body biogenesis and inheritance in this early divergent eukaryote. IMPORTANCE The basal body in the early-branching protozoan Trypanosoma brucei nucleates flagellum assembly and also regulates organelle segregation, cell morphogenesis, and cell division. However, the molecular composition and the assembly process of the basal body remain poorly understood. Here, we identify 14 conserved basal body proteins and 25 trypanosome-specific basal body proteins via bioinformatics, localization-based screening, and proximity-dependent biotin identification. We further localized these proteins to distinct subdomains of the basal body by using fluorescence microscopy and superresolution microscopy, discovered novel regulators of basal body duplication and separation, and uncovered new functions of conserved basal body proteins in basal body duplication and separation. This work lays the foundation for dissecting the mechanisms underlying basal body biogenesis and inheritance in T. brucei.
format	article
author	Hung Quang Dang Qing Zhou Veronica W. Rowlett Huiqing Hu Kyu Joon Lee William Margolin Ziyin Li
author_facet	Hung Quang Dang Qing Zhou Veronica W. Rowlett Huiqing Hu Kyu Joon Lee William Margolin Ziyin Li
author_sort	Hung Quang Dang
title	Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance
title_short	Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance
title_full	Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance
title_fullStr	Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance
title_full_unstemmed	Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance
title_sort	proximity interactions among basal body components in <italic toggle="yes">trypanosoma brucei</italic> identify novel regulators of basal body biogenesis and inheritance
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/74af67cc051d4202bd3f0164ad2c2d68
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Proximity Interactions among Basal Body Components in <italic toggle="yes">Trypanosoma brucei</italic> Identify Novel Regulators of Basal Body Biogenesis and Inheritance

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