Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers

Background. Tissue repair processes are impaired in diabetic foot ulcers (DFUs). Previous research has shown that glycaemic control, cytokines and growth factors play an important role in wound healing. Emerging evidence also suggests that genes play a role via their regulation of cell proliferation...

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Autores principales:	Ekaterina L. Zaitseva, Alla Y. Tokmakova, Iya A. Voronkova, Vasily M. Petrov, Anatoly N. Tiulpakov, Marina V. Shestakova
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2017
Materias:	diabetes mellitus wounds dfus repair genes expression Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/74bb9c76596c45d88f1681a29b7d59fd
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spelling	oai:doaj.org-article:74bb9c76596c45d88f1681a29b7d59fd2021-11-14T09:00:21ZGenetic parameters of wound healing in patients with neuropatic diabetic foot ulcers2072-03512072-037810.14341/DM9291https://doaj.org/article/74bb9c76596c45d88f1681a29b7d59fd2017-12-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/9291https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Background. Tissue repair processes are impaired in diabetic foot ulcers (DFUs). Previous research has shown that glycaemic control, cytokines and growth factors play an important role in wound healing. Emerging evidence also suggests that genes play a role via their regulation of cell proliferation, collagen synthesis and granulation tissue formation. Aim. To evaluate collagen genes expression in different stages of wound healing in patients with DFUs. Materials and methods. Prospective study included four patients with neuropathic DFUs after surgical debridement. Tissue samples were taken for morphological and genetic tests on days 0, 10 and 15 of local treatment to evaluate expression of collagen genes (i.e. COL1A1, COL1A2, COL3A1) and to perform morphological tests. Results. The present study confirmed that the size of wounds decreased by 8.8 ± 7% after 10 days of local treatment and by 18.3 ± 8% after 15 days of local treatment. According to histological examination of wound biopsies at day 10, all patients showed a tendency for lower levels of inflammatory infiltrate, increased number of fibroblast-like cells, presence of maturing granulation tissue and emergence of connective tissue fibres. After 15 days, we detected inflammatory infiltration in the wounds, despite the formation of mature granulation tissue. According to results of genetic analysis on day 10 of local wound treatment, we found a tendency for increased expression of collagen genes relative to the baseline: COL1A1 increased by 3.2 ± 1.3 times, COL1A2 by 2.0 ± 1.0 times and COL3A1 by 1.25 ± 1.1 times. On day 15 of local treatment, in contrast, we found a tendency for decreased expression of COL1A1, COL1A2 and COL3A1 relative to the baseline (1.7 ± 0.6, 2.5 ± 2 and 20.0 ± 3 times, respectively). Conclusions. The expression of collagen genes (COL1A1, COL1A2, COL3A1) is more pronounced in proliferation phase and is subsequently reduced towards the end. These data were confirmed by morphological study and clinical pictures.Ekaterina L. ZaitsevaAlla Y. TokmakovaIya A. VoronkovaVasily M. PetrovAnatoly N. TiulpakovMarina V. ShestakovaEndocrinology Research Centrearticlediabetes mellituswoundsdfusrepairgenesexpressionNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 20, Iss 5, Pp 344-349 (2017)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	diabetes mellitus wounds dfus repair genes expression Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	diabetes mellitus wounds dfus repair genes expression Nutritional diseases. Deficiency diseases RC620-627 Ekaterina L. Zaitseva Alla Y. Tokmakova Iya A. Voronkova Vasily M. Petrov Anatoly N. Tiulpakov Marina V. Shestakova Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
description	Background. Tissue repair processes are impaired in diabetic foot ulcers (DFUs). Previous research has shown that glycaemic control, cytokines and growth factors play an important role in wound healing. Emerging evidence also suggests that genes play a role via their regulation of cell proliferation, collagen synthesis and granulation tissue formation. Aim. To evaluate collagen genes expression in different stages of wound healing in patients with DFUs. Materials and methods. Prospective study included four patients with neuropathic DFUs after surgical debridement. Tissue samples were taken for morphological and genetic tests on days 0, 10 and 15 of local treatment to evaluate expression of collagen genes (i.e. COL1A1, COL1A2, COL3A1) and to perform morphological tests. Results. The present study confirmed that the size of wounds decreased by 8.8 ± 7% after 10 days of local treatment and by 18.3 ± 8% after 15 days of local treatment. According to histological examination of wound biopsies at day 10, all patients showed a tendency for lower levels of inflammatory infiltrate, increased number of fibroblast-like cells, presence of maturing granulation tissue and emergence of connective tissue fibres. After 15 days, we detected inflammatory infiltration in the wounds, despite the formation of mature granulation tissue. According to results of genetic analysis on day 10 of local wound treatment, we found a tendency for increased expression of collagen genes relative to the baseline: COL1A1 increased by 3.2 ± 1.3 times, COL1A2 by 2.0 ± 1.0 times and COL3A1 by 1.25 ± 1.1 times. On day 15 of local treatment, in contrast, we found a tendency for decreased expression of COL1A1, COL1A2 and COL3A1 relative to the baseline (1.7 ± 0.6, 2.5 ± 2 and 20.0 ± 3 times, respectively). Conclusions. The expression of collagen genes (COL1A1, COL1A2, COL3A1) is more pronounced in proliferation phase and is subsequently reduced towards the end. These data were confirmed by morphological study and clinical pictures.
format	article
author	Ekaterina L. Zaitseva Alla Y. Tokmakova Iya A. Voronkova Vasily M. Petrov Anatoly N. Tiulpakov Marina V. Shestakova
author_facet	Ekaterina L. Zaitseva Alla Y. Tokmakova Iya A. Voronkova Vasily M. Petrov Anatoly N. Tiulpakov Marina V. Shestakova
author_sort	Ekaterina L. Zaitseva
title	Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
title_short	Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
title_full	Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
title_fullStr	Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
title_full_unstemmed	Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
title_sort	genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers
publisher	Endocrinology Research Centre
publishDate	2017
url	https://doaj.org/article/74bb9c76596c45d88f1681a29b7d59fd
work_keys_str_mv	AT ekaterinalzaitseva geneticparametersofwoundhealinginpatientswithneuropaticdiabeticfootulcers AT allaytokmakova geneticparametersofwoundhealinginpatientswithneuropaticdiabeticfootulcers AT iyaavoronkova geneticparametersofwoundhealinginpatientswithneuropaticdiabeticfootulcers AT vasilympetrov geneticparametersofwoundhealinginpatientswithneuropaticdiabeticfootulcers AT anatolyntiulpakov geneticparametersofwoundhealinginpatientswithneuropaticdiabeticfootulcers AT marinavshestakova geneticparametersofwoundhealinginpatientswithneuropaticdiabeticfootulcers
_version_	1718429539191750656

Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers

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