Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran

Background: Toxoplasma gondii, an obligate intracellular protozoan, causes toxoplasmosis. The aim of this study was molecular detection of T. gondii in breast and goat milk samples by the molecular method in the central Iran. Methods: Totally, 300 human' and 200 goats' milk samples were...

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Autores principales:	Esmat Mohammadi Khamsian, Bahador Hajimohammadi, Gilda Eslami, Mohammad Hossein Fallahzadeh, Saeede Sadat Hosseini
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	toxoplasmosis milk human goats polymerase chain reaction Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/74beec197fbd4c90b94a7ea41b7275d1
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spelling	oai:doaj.org-article:74beec197fbd4c90b94a7ea41b7275d12021-11-29T07:18:54ZToxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran1735-70202008-238Xhttps://doaj.org/article/74beec197fbd4c90b94a7ea41b7275d12021-11-01T00:00:00Zhttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/3194https://doaj.org/toc/1735-7020https://doaj.org/toc/2008-238XBackground: Toxoplasma gondii, an obligate intracellular protozoan, causes toxoplasmosis. The aim of this study was molecular detection of T. gondii in breast and goat milk samples by the molecular method in the central Iran. Methods: Totally, 300 human' and 200 goats' milk samples were collected randomly from different regions of central Iran in 2018. DNA extraction was performed by the salting-out method. Molecular detection of the parasite was done by nested-PCR using the specific primer pairs. Statistical analysis was performed by SPSS 23 using descriptive and Chi-square tests. Results: Out of 300 human milk samples, 1 sample (0.3%) was infected with T. gondii. Out of 200 samples of goat milk, 11 samples (5.5%) showed infection with T. gondii. The frequency of infection in goat's milk samples was 4.36% in the south and west, 1.9% in northern regions, and 2% in eastern regions of the province. The statistical analysis showed no significant difference between toxoplasmosis and different geographical regions in the province. Conclusion: Because of the popularity of the goat milk and the transfection probability with the milk to humans, it is recommended to boil milk prior to use. Furthermore, case contamination of T. gondii in the human milk sample showed one of the important paths for infection transmission, which requires further studies.Esmat Mohammadi KhamsianBahador HajimohammadiGilda EslamiMohammad Hossein FallahzadehSaeede Sadat HosseiniTehran University of Medical Sciencesarticletoxoplasmosismilkhumangoatspolymerase chain reactionInfectious and parasitic diseasesRC109-216ENIranian Journal of Parasitology, Vol 16, Iss 4, Pp 601-609 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	toxoplasmosis milk human goats polymerase chain reaction Infectious and parasitic diseases RC109-216
spellingShingle	toxoplasmosis milk human goats polymerase chain reaction Infectious and parasitic diseases RC109-216 Esmat Mohammadi Khamsian Bahador Hajimohammadi Gilda Eslami Mohammad Hossein Fallahzadeh Saeede Sadat Hosseini Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran
description	Background: Toxoplasma gondii, an obligate intracellular protozoan, causes toxoplasmosis. The aim of this study was molecular detection of T. gondii in breast and goat milk samples by the molecular method in the central Iran. Methods: Totally, 300 human' and 200 goats' milk samples were collected randomly from different regions of central Iran in 2018. DNA extraction was performed by the salting-out method. Molecular detection of the parasite was done by nested-PCR using the specific primer pairs. Statistical analysis was performed by SPSS 23 using descriptive and Chi-square tests. Results: Out of 300 human milk samples, 1 sample (0.3%) was infected with T. gondii. Out of 200 samples of goat milk, 11 samples (5.5%) showed infection with T. gondii. The frequency of infection in goat's milk samples was 4.36% in the south and west, 1.9% in northern regions, and 2% in eastern regions of the province. The statistical analysis showed no significant difference between toxoplasmosis and different geographical regions in the province. Conclusion: Because of the popularity of the goat milk and the transfection probability with the milk to humans, it is recommended to boil milk prior to use. Furthermore, case contamination of T. gondii in the human milk sample showed one of the important paths for infection transmission, which requires further studies.
format	article
author	Esmat Mohammadi Khamsian Bahador Hajimohammadi Gilda Eslami Mohammad Hossein Fallahzadeh Saeede Sadat Hosseini
author_facet	Esmat Mohammadi Khamsian Bahador Hajimohammadi Gilda Eslami Mohammad Hossein Fallahzadeh Saeede Sadat Hosseini
author_sort	Esmat Mohammadi Khamsian
title	Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran
title_short	Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran
title_full	Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran
title_fullStr	Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran
title_full_unstemmed	Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran
title_sort	toxoplasma gondii in milk of human and goat from the desert area in central iran
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/74beec197fbd4c90b94a7ea41b7275d1
work_keys_str_mv	AT esmatmohammadikhamsian toxoplasmagondiiinmilkofhumanandgoatfromthedesertareaincentraliran AT bahadorhajimohammadi toxoplasmagondiiinmilkofhumanandgoatfromthedesertareaincentraliran AT gildaeslami toxoplasmagondiiinmilkofhumanandgoatfromthedesertareaincentraliran AT mohammadhosseinfallahzadeh toxoplasmagondiiinmilkofhumanandgoatfromthedesertareaincentraliran AT saeedesadathosseini toxoplasmagondiiinmilkofhumanandgoatfromthedesertareaincentraliran
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Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran

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