Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi

Abstract Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes cv. V...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tatjana Ćosić, Václav Motyka, Jelena Savić, Martin Raspor, Marija Marković, Petre I. Dobrev, Slavica Ninković
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/74c4d62b312842258a991c1ce6e58d24
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:74c4d62b312842258a991c1ce6e58d24
record_format	dspace
spelling	oai:doaj.org-article:74c4d62b312842258a991c1ce6e58d242021-12-02T13:24:25ZSucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi10.1038/s41598-021-85932-w2045-2322https://doaj.org/article/74c4d62b312842258a991c1ce6e58d242021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85932-whttps://doaj.org/toc/2045-2322Abstract Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for transZ treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but transZ induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.Tatjana ĆosićVáclav MotykaJelena SavićMartin RasporMarija MarkovićPetre I. DobrevSlavica NinkovićNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatjana Ćosić Václav Motyka Jelena Savić Martin Raspor Marija Marković Petre I. Dobrev Slavica Ninković Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
description	Abstract Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for transZ treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but transZ induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
format	article
author	Tatjana Ćosić Václav Motyka Jelena Savić Martin Raspor Marija Marković Petre I. Dobrev Slavica Ninković
author_facet	Tatjana Ćosić Václav Motyka Jelena Savić Martin Raspor Marija Marković Petre I. Dobrev Slavica Ninković
author_sort	Tatjana Ćosić
title	Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
title_short	Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
title_full	Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
title_fullStr	Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
title_full_unstemmed	Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
title_sort	sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/74c4d62b312842258a991c1ce6e58d24
work_keys_str_mv	AT tatjanacosic sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi AT vaclavmotyka sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi AT jelenasavic sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi AT martinraspor sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi AT marijamarkovic sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi AT petreidobrev sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi AT slavicaninkovic sucroseinterfereswithendogenouscytokininhomeostasisandexpressionoforganogenesisrelatedgenesduringdenovoshootorganogenesisinkohlrabi
_version_	1718393102385807360

Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi

Ejemplares similares