Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells

Tissue extracellular matrix (ECM) is a structurally and compositionally unique microenvironment within which native cells can perform their natural biological activities. Cells grown on artificial substrata differ biologically and phenotypically from those grown within their native tissue microenvir...

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Autores principales:	Chandler R. Keller, Yang Hu, Kelsey F. Ruud, Anika E. VanDeen, Steve R. Martinez, Barry T. Kahn, Zhiwu Zhang, Roland K. Chen, Weimin Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	breast tissue extracellular matrix structure hydrogel microenvironment breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/74c800c385bb450a84aea351dd39f160
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spelling	oai:doaj.org-article:74c800c385bb450a84aea351dd39f1602021-11-25T17:04:47ZHuman Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells10.3390/cancers132258572072-6694https://doaj.org/article/74c800c385bb450a84aea351dd39f1602021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5857https://doaj.org/toc/2072-6694Tissue extracellular matrix (ECM) is a structurally and compositionally unique microenvironment within which native cells can perform their natural biological activities. Cells grown on artificial substrata differ biologically and phenotypically from those grown within their native tissue microenvironment. Studies examining human tissue ECM structures and the biology of human tissue cells in their corresponding tissue ECM are lacking. Such investigations will improve our understanding about human pathophysiological conditions for better clinical care. We report here human normal breast tissue and invasive ductal carcinoma tissue ECM structural features. For the first time, a hydrogel was successfully fabricated using whole protein extracts of human normal breast ECM. Using immunofluorescence staining of type I collagen (Col I) and machine learning of its fibrous patterns in the polymerized human breast ECM hydrogel, we have defined the microstructural characteristics of the hydrogel and compared the microstructures with those of other native ECM hydrogels. Importantly, the ECM hydrogel supported 3D growth and cell-ECM interaction of both normal and cancerous mammary epithelial cells. This work represents further advancement toward full reconstitution of the human breast tissue microenvironment, an accomplishment that will accelerate the use of human pathophysiological tissue-derived matrices for individualized biomedical research and therapeutic development.Chandler R. KellerYang HuKelsey F. RuudAnika E. VanDeenSteve R. MartinezBarry T. KahnZhiwu ZhangRoland K. ChenWeimin LiMDPI AGarticlebreast tissueextracellular matrixstructurehydrogelmicroenvironmentbreast cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5857, p 5857 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast tissue extracellular matrix structure hydrogel microenvironment breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	breast tissue extracellular matrix structure hydrogel microenvironment breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Chandler R. Keller Yang Hu Kelsey F. Ruud Anika E. VanDeen Steve R. Martinez Barry T. Kahn Zhiwu Zhang Roland K. Chen Weimin Li Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
description	Tissue extracellular matrix (ECM) is a structurally and compositionally unique microenvironment within which native cells can perform their natural biological activities. Cells grown on artificial substrata differ biologically and phenotypically from those grown within their native tissue microenvironment. Studies examining human tissue ECM structures and the biology of human tissue cells in their corresponding tissue ECM are lacking. Such investigations will improve our understanding about human pathophysiological conditions for better clinical care. We report here human normal breast tissue and invasive ductal carcinoma tissue ECM structural features. For the first time, a hydrogel was successfully fabricated using whole protein extracts of human normal breast ECM. Using immunofluorescence staining of type I collagen (Col I) and machine learning of its fibrous patterns in the polymerized human breast ECM hydrogel, we have defined the microstructural characteristics of the hydrogel and compared the microstructures with those of other native ECM hydrogels. Importantly, the ECM hydrogel supported 3D growth and cell-ECM interaction of both normal and cancerous mammary epithelial cells. This work represents further advancement toward full reconstitution of the human breast tissue microenvironment, an accomplishment that will accelerate the use of human pathophysiological tissue-derived matrices for individualized biomedical research and therapeutic development.
format	article
author	Chandler R. Keller Yang Hu Kelsey F. Ruud Anika E. VanDeen Steve R. Martinez Barry T. Kahn Zhiwu Zhang Roland K. Chen Weimin Li
author_facet	Chandler R. Keller Yang Hu Kelsey F. Ruud Anika E. VanDeen Steve R. Martinez Barry T. Kahn Zhiwu Zhang Roland K. Chen Weimin Li
author_sort	Chandler R. Keller
title	Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
title_short	Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
title_full	Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
title_fullStr	Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
title_full_unstemmed	Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells
title_sort	human breast extracellular matrix microstructures and protein hydrogel 3d cultures of mammary epithelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/74c800c385bb450a84aea351dd39f160
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Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells

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