Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons

Abstract Insight into the bidirectional signaling between primary human myogenic cells and neurons is lacking. For this purpose, human myogenic cells were derived from the semitendinosus and gracilis muscles of five healthy individuals and co‐cultured with cerebellar granule neurons from two litters...

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Autores principales:	Michal Tamáš, Stanislava Pankratova, Peter Schjerling, Casper Soendenbroe, Ching‐Yan Chloé Yeung, Cristian Pablo Pennisi, Jens R. Jakobsen, Michael R. Krogsgaard, Michael Kjaer, Abigail L. Mackey
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	acetylcholine receptor innervation myogenesis neuron skeletal muscle Physiology QP1-981
Acceso en línea:	https://doaj.org/article/74cb20f03038431e9c90762e3b383d57
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spelling	oai:doaj.org-article:74cb20f03038431e9c90762e3b383d572021-11-15T09:54:40ZMutual stimulatory signaling between human myogenic cells and rat cerebellar neurons2051-817X10.14814/phy2.15077https://doaj.org/article/74cb20f03038431e9c90762e3b383d572021-11-01T00:00:00Zhttps://doi.org/10.14814/phy2.15077https://doaj.org/toc/2051-817XAbstract Insight into the bidirectional signaling between primary human myogenic cells and neurons is lacking. For this purpose, human myogenic cells were derived from the semitendinosus and gracilis muscles of five healthy individuals and co‐cultured with cerebellar granule neurons from two litters of 7‐day‐old Wistar rat pups, in muscle medium or neural medium, alongside monocultures of myogenic cells or neurons. RT‐PCR was performed to determine human mRNA levels of GAPDH, Ki67, myogenin, and MUSK, and the acetylcholine receptor subtypes CHRNA1, CHRNB1, CHRNG, CHRND, and CHRNE, and rat mRNA levels of GAPDH, Fth1, Rack1, vimentin, Cdh13, and Ppp1r1a. Immunocytochemistry was used to evaluate neurite outgrowth (GAP43) in the presence and absence of myogenic cells. Co‐culture with primary neurons lead to higher myogenic cell gene expression levels of GAPDH, myogenin, MUSK, CHRNA1, CHRNG, and CHRND, compared to myogenic cells cultured alone. It appeared that neurons preferentially attached to myotubes and that neurite outgrowth was enhanced when neurons were cultured with myogenic cells compared to monoculture. In neural medium, rat mRNA levels of GAPDH, vimentin, Cdh13, and Ppp1r1a were greater in co‐culture, versus monoculture, whereas in muscle medium co‐culture lead to lower levels of Fth1, Rack1, vimentin, and Cdh13 than monoculture. These findings demonstrate mutually beneficial stimulatory signaling between rat cerebellar granule neurons and human myogenic cells, providing support for an active role for both the neuron and the muscle cell in stimulating neurite growth and myogenesis. Bidirectional muscle nerve signaling.Michal TamášStanislava PankratovaPeter SchjerlingCasper SoendenbroeChing‐Yan Chloé YeungCristian Pablo PennisiJens R. JakobsenMichael R. KrogsgaardMichael KjaerAbigail L. MackeyWileyarticleacetylcholine receptorinnervationmyogenesisneuronskeletal musclePhysiologyQP1-981ENPhysiological Reports, Vol 9, Iss 21, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acetylcholine receptor innervation myogenesis neuron skeletal muscle Physiology QP1-981
spellingShingle	acetylcholine receptor innervation myogenesis neuron skeletal muscle Physiology QP1-981 Michal Tamáš Stanislava Pankratova Peter Schjerling Casper Soendenbroe Ching‐Yan Chloé Yeung Cristian Pablo Pennisi Jens R. Jakobsen Michael R. Krogsgaard Michael Kjaer Abigail L. Mackey Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
description	Abstract Insight into the bidirectional signaling between primary human myogenic cells and neurons is lacking. For this purpose, human myogenic cells were derived from the semitendinosus and gracilis muscles of five healthy individuals and co‐cultured with cerebellar granule neurons from two litters of 7‐day‐old Wistar rat pups, in muscle medium or neural medium, alongside monocultures of myogenic cells or neurons. RT‐PCR was performed to determine human mRNA levels of GAPDH, Ki67, myogenin, and MUSK, and the acetylcholine receptor subtypes CHRNA1, CHRNB1, CHRNG, CHRND, and CHRNE, and rat mRNA levels of GAPDH, Fth1, Rack1, vimentin, Cdh13, and Ppp1r1a. Immunocytochemistry was used to evaluate neurite outgrowth (GAP43) in the presence and absence of myogenic cells. Co‐culture with primary neurons lead to higher myogenic cell gene expression levels of GAPDH, myogenin, MUSK, CHRNA1, CHRNG, and CHRND, compared to myogenic cells cultured alone. It appeared that neurons preferentially attached to myotubes and that neurite outgrowth was enhanced when neurons were cultured with myogenic cells compared to monoculture. In neural medium, rat mRNA levels of GAPDH, vimentin, Cdh13, and Ppp1r1a were greater in co‐culture, versus monoculture, whereas in muscle medium co‐culture lead to lower levels of Fth1, Rack1, vimentin, and Cdh13 than monoculture. These findings demonstrate mutually beneficial stimulatory signaling between rat cerebellar granule neurons and human myogenic cells, providing support for an active role for both the neuron and the muscle cell in stimulating neurite growth and myogenesis. Bidirectional muscle nerve signaling.
format	article
author	Michal Tamáš Stanislava Pankratova Peter Schjerling Casper Soendenbroe Ching‐Yan Chloé Yeung Cristian Pablo Pennisi Jens R. Jakobsen Michael R. Krogsgaard Michael Kjaer Abigail L. Mackey
author_facet	Michal Tamáš Stanislava Pankratova Peter Schjerling Casper Soendenbroe Ching‐Yan Chloé Yeung Cristian Pablo Pennisi Jens R. Jakobsen Michael R. Krogsgaard Michael Kjaer Abigail L. Mackey
author_sort	Michal Tamáš
title	Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
title_short	Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
title_full	Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
title_fullStr	Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
title_full_unstemmed	Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
title_sort	mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/74cb20f03038431e9c90762e3b383d57
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_version_	1718428442419003392

Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons

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