Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke

Introduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Conita Yuniarifa, Joko Wahyu Wibowo, Taufiqurrachman Nasihun
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2021
Materias:	sertoli and leydig cells, sperm quality, expression of caspase-3 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/74cdfe559edb403bb8bf8eba503e3c23
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:74cdfe559edb403bb8bf8eba503e3c23
record_format	dspace
spelling	oai:doaj.org-article:74cdfe559edb403bb8bf8eba503e3c232021-11-25T13:16:44ZEffect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke2085-15452339-093X10.30659/sainsmed.v11i2.2465https://doaj.org/article/74cdfe559edb403bb8bf8eba503e3c232021-01-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/2465https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods: This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results: There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3. Conclusion:The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .Conita YuniarifaJoko Wahyu WibowoTaufiqurrachman NasihunUniversitas Sultan Agung Semarangarticlesertoli and leydig cells, sperm quality, expression of caspase-3Medicine (General)R5-920ENSains Medika, Vol 11, Iss 2, Pp 81-88 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sertoli and leydig cells, sperm quality, expression of caspase-3 Medicine (General) R5-920
spellingShingle	sertoli and leydig cells, sperm quality, expression of caspase-3 Medicine (General) R5-920 Conita Yuniarifa Joko Wahyu Wibowo Taufiqurrachman Nasihun Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
description	Introduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods: This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results: There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3. Conclusion:The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .
format	article
author	Conita Yuniarifa Joko Wahyu Wibowo Taufiqurrachman Nasihun
author_facet	Conita Yuniarifa Joko Wahyu Wibowo Taufiqurrachman Nasihun
author_sort	Conita Yuniarifa
title	Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
title_short	Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
title_full	Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
title_fullStr	Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
title_full_unstemmed	Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
title_sort	effect of four antioxidants combination on number of sertoli and leydig cells, sperm quality and caspase-3 expression in rat exposed to cigarette smoke
publisher	Universitas Sultan Agung Semarang
publishDate	2021
url	https://doaj.org/article/74cdfe559edb403bb8bf8eba503e3c23
work_keys_str_mv	AT conitayuniarifa effectoffourantioxidantscombinationonnumberofsertoliandleydigcellsspermqualityandcaspase3expressioninratexposedtocigarettesmoke AT jokowahyuwibowo effectoffourantioxidantscombinationonnumberofsertoliandleydigcellsspermqualityandcaspase3expressioninratexposedtocigarettesmoke AT taufiqurrachmannasihun effectoffourantioxidantscombinationonnumberofsertoliandleydigcellsspermqualityandcaspase3expressioninratexposedtocigarettesmoke
_version_	1718413503062081536

Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke

Ejemplares similares