Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways

Abstract Shikonin is the main component of the traditional Chinese medicine comfrey, which can inhibit the activity of PKM2 by regulating glycolysis and ATP production. Rheumatoid arthritis synovial cells (RA-FLSs) have been reported to increase glycolytic activity and have other similar hallmarks o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiahui Li, Jinglong Pang, Zhe Liu, XianMing Ge, Yanan Zhen, Chen Chen Jiang, Yaming Liu, Qiang Huo, Yiming Sun, Hao Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/74d4539214384343a32c81d3712be7cf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:74d4539214384343a32c81d3712be7cf
record_format	dspace
spelling	oai:doaj.org-article:74d4539214384343a32c81d3712be7cf2021-12-02T18:02:23ZShikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways10.1038/s41598-021-97713-62045-2322https://doaj.org/article/74d4539214384343a32c81d3712be7cf2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97713-6https://doaj.org/toc/2045-2322Abstract Shikonin is the main component of the traditional Chinese medicine comfrey, which can inhibit the activity of PKM2 by regulating glycolysis and ATP production. Rheumatoid arthritis synovial cells (RA-FLSs) have been reported to increase glycolytic activity and have other similar hallmarks of metabolic activity. In this study, we investigated the effects of shikonin on glycolysis, mitochondrial function, and cell death in RA-FLSs. The results showed that shikonin induced apoptosis and autophagy in RA-FLSs by activating the production of reactive oxygen species (ROS) and inhibiting intracellular ATP levels, glycolysis-related proteins, and the PI3K-AKT-mTOR signaling pathway. Shikonin can significantly reduce the expression of apoptosis-related proteins, paw swelling in rat arthritic tissues, and the levels of inflammatory factors in peripheral blood, such as TNF-α, IL-6, IL-8, IL-10, IL-17A, and IL-1β while showing less toxicity to the liver and kidney.Jiahui LiJinglong PangZhe LiuXianMing GeYanan ZhenChen Chen JiangYaming LiuQiang HuoYiming SunHao LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiahui Li Jinglong Pang Zhe Liu XianMing Ge Yanan Zhen Chen Chen Jiang Yaming Liu Qiang Huo Yiming Sun Hao Liu Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
description	Abstract Shikonin is the main component of the traditional Chinese medicine comfrey, which can inhibit the activity of PKM2 by regulating glycolysis and ATP production. Rheumatoid arthritis synovial cells (RA-FLSs) have been reported to increase glycolytic activity and have other similar hallmarks of metabolic activity. In this study, we investigated the effects of shikonin on glycolysis, mitochondrial function, and cell death in RA-FLSs. The results showed that shikonin induced apoptosis and autophagy in RA-FLSs by activating the production of reactive oxygen species (ROS) and inhibiting intracellular ATP levels, glycolysis-related proteins, and the PI3K-AKT-mTOR signaling pathway. Shikonin can significantly reduce the expression of apoptosis-related proteins, paw swelling in rat arthritic tissues, and the levels of inflammatory factors in peripheral blood, such as TNF-α, IL-6, IL-8, IL-10, IL-17A, and IL-1β while showing less toxicity to the liver and kidney.
format	article
author	Jiahui Li Jinglong Pang Zhe Liu XianMing Ge Yanan Zhen Chen Chen Jiang Yaming Liu Qiang Huo Yiming Sun Hao Liu
author_facet	Jiahui Li Jinglong Pang Zhe Liu XianMing Ge Yanan Zhen Chen Chen Jiang Yaming Liu Qiang Huo Yiming Sun Hao Liu
author_sort	Jiahui Li
title	Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
title_short	Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
title_full	Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
title_fullStr	Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
title_full_unstemmed	Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
title_sort	shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/74d4539214384343a32c81d3712be7cf
work_keys_str_mv	AT jiahuili shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT jinglongpang shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT zheliu shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT xianmingge shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT yananzhen shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT chenchenjiang shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT yamingliu shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT qianghuo shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT yimingsun shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways AT haoliu shikonininducesprogrammeddeathoffibroblastsynovialcellsinrheumatoidarthritisbyinhibitingenergypathways
_version_	1718378933932523520

Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways

Ejemplares similares