Paramutation-like interaction of T-DNA loci in Arabidopsis.

In paramutation, epigenetic information is transferred from one allele to another to create a gene expression state which is stably inherited over generations. Typically, paramutation describes a phenomenon where one allele of a gene down-regulates the expression of another allele. Paramutation has...

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Autores principales:	Weiya Xue, Colin Ruprecht, Nathaniel Street, Kian Hematy, Christine Chang, Wolf B Frommer, Staffan Persson, Totte Niittylä
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/74dd2d298d2143a593ca3fed4d6619f9
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spelling	oai:doaj.org-article:74dd2d298d2143a593ca3fed4d6619f92021-11-18T08:04:55ZParamutation-like interaction of T-DNA loci in Arabidopsis.1932-620310.1371/journal.pone.0051651https://doaj.org/article/74dd2d298d2143a593ca3fed4d6619f92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23272131/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In paramutation, epigenetic information is transferred from one allele to another to create a gene expression state which is stably inherited over generations. Typically, paramutation describes a phenomenon where one allele of a gene down-regulates the expression of another allele. Paramutation has been described in several eukaryotes and is best understood in plants. Here we describe an unexpected paramutation-like trans SALK T-DNA interaction in Arabidopsis. Unlike most of the previously described paramutations, which led to gene silencing, the trans SALK T-DNA interaction caused an increase in the transcript levels of the endogenous gene (COBRA) where the T-DNA was inserted. This increased COBRA expression state was stably inherited for several generations and led to the partial suppression of the cobra phenotype. DNA methylation was implicated in this trans SALK T-DNA interaction since mutation of the DNA methyltransferase 1 in the suppressed cobra caused a reversal of the suppression. In addition, null mutants of the DNA demethylase ROS1 caused a similar COBRA transcript increase in the cobra SALK T-DNA mutant as the trans T-DNA interaction. Our results provide a new example of a paramutation-like trans T-DNA interaction in Arabidopsis, and establish a convenient hypocotyl elongation assay to study this phenomenon. The results also alert to the possibility of unexpected endogenous transcript increase when two T-DNAs are combined in the same genetic background.Weiya XueColin RuprechtNathaniel StreetKian HematyChristine ChangWolf B FrommerStaffan PerssonTotte NiittyläPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51651 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Weiya Xue Colin Ruprecht Nathaniel Street Kian Hematy Christine Chang Wolf B Frommer Staffan Persson Totte Niittylä Paramutation-like interaction of T-DNA loci in Arabidopsis.
description	In paramutation, epigenetic information is transferred from one allele to another to create a gene expression state which is stably inherited over generations. Typically, paramutation describes a phenomenon where one allele of a gene down-regulates the expression of another allele. Paramutation has been described in several eukaryotes and is best understood in plants. Here we describe an unexpected paramutation-like trans SALK T-DNA interaction in Arabidopsis. Unlike most of the previously described paramutations, which led to gene silencing, the trans SALK T-DNA interaction caused an increase in the transcript levels of the endogenous gene (COBRA) where the T-DNA was inserted. This increased COBRA expression state was stably inherited for several generations and led to the partial suppression of the cobra phenotype. DNA methylation was implicated in this trans SALK T-DNA interaction since mutation of the DNA methyltransferase 1 in the suppressed cobra caused a reversal of the suppression. In addition, null mutants of the DNA demethylase ROS1 caused a similar COBRA transcript increase in the cobra SALK T-DNA mutant as the trans T-DNA interaction. Our results provide a new example of a paramutation-like trans T-DNA interaction in Arabidopsis, and establish a convenient hypocotyl elongation assay to study this phenomenon. The results also alert to the possibility of unexpected endogenous transcript increase when two T-DNAs are combined in the same genetic background.
format	article
author	Weiya Xue Colin Ruprecht Nathaniel Street Kian Hematy Christine Chang Wolf B Frommer Staffan Persson Totte Niittylä
author_facet	Weiya Xue Colin Ruprecht Nathaniel Street Kian Hematy Christine Chang Wolf B Frommer Staffan Persson Totte Niittylä
author_sort	Weiya Xue
title	Paramutation-like interaction of T-DNA loci in Arabidopsis.
title_short	Paramutation-like interaction of T-DNA loci in Arabidopsis.
title_full	Paramutation-like interaction of T-DNA loci in Arabidopsis.
title_fullStr	Paramutation-like interaction of T-DNA loci in Arabidopsis.
title_full_unstemmed	Paramutation-like interaction of T-DNA loci in Arabidopsis.
title_sort	paramutation-like interaction of t-dna loci in arabidopsis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/74dd2d298d2143a593ca3fed4d6619f9
work_keys_str_mv	AT weiyaxue paramutationlikeinteractionoftdnalociinarabidopsis AT colinruprecht paramutationlikeinteractionoftdnalociinarabidopsis AT nathanielstreet paramutationlikeinteractionoftdnalociinarabidopsis AT kianhematy paramutationlikeinteractionoftdnalociinarabidopsis AT christinechang paramutationlikeinteractionoftdnalociinarabidopsis AT wolfbfrommer paramutationlikeinteractionoftdnalociinarabidopsis AT staffanpersson paramutationlikeinteractionoftdnalociinarabidopsis AT totteniittyla paramutationlikeinteractionoftdnalociinarabidopsis
_version_	1718422234853277696

Paramutation-like interaction of T-DNA loci in Arabidopsis.

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