Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai

Scyphozoan Nemopilema nomurai envenomation is an unresolved threat to human health in Asian waters. Nemopilema nomurai venom metalloproteinases show important toxicities in skin damage and inflammation, but there is still no purified protein for further studies. In this study, high proteinase activi...

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Autores principales:	Chunlin Yu, Rongfeng Li, Xiujing Yin, Huahua Yu, Pengcheng Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	jellyfish Nemopilema nomurai purification protease metalloprotease synergistic effect Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/74df05ad92184c84a95cef9cbbd819c9
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spelling	oai:doaj.org-article:74df05ad92184c84a95cef9cbbd819c92021-12-01T02:22:18ZSynergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai1663-981210.3389/fphar.2021.791847https://doaj.org/article/74df05ad92184c84a95cef9cbbd819c92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.791847/fullhttps://doaj.org/toc/1663-9812Scyphozoan Nemopilema nomurai envenomation is an unresolved threat to human health in Asian waters. Nemopilema nomurai venom metalloproteinases show important toxicities in skin damage and inflammation, but there is still no purified protein for further studies. In this study, high proteinase activity fractions in tentacle autolysis were isolated by ammonium sulfate precipitation, DEAE Sepharose Fast Flow, and Superdex 75 chromatography successively. Purification was guided by azocasein hydrolysis activity and SDS-PAGE. The final products were analyzed by LC-MS/MS. Four elution peaks purified by Superdex 75 chromatography had multiple protein bands but did not show proteinase activity. These fractions would recover proteinase activity after mixing again. Regulation mechanisms were speculated as binding metalloproteinase regulator or disaggregating metalloproteinase inhibitor by LC-MS/MS analysis. For the first time, a synergistic effect in N. nomurai proteinase activity was found in the purification process.Chunlin YuChunlin YuRongfeng LiRongfeng LiXiujing YinXiujing YinHuahua YuHuahua YuPengcheng LiPengcheng LiFrontiers Media S.A.articlejellyfish Nemopilema nomuraipurificationproteasemetalloproteasesynergistic effectTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	jellyfish Nemopilema nomurai purification protease metalloprotease synergistic effect Therapeutics. Pharmacology RM1-950
spellingShingle	jellyfish Nemopilema nomurai purification protease metalloprotease synergistic effect Therapeutics. Pharmacology RM1-950 Chunlin Yu Chunlin Yu Rongfeng Li Rongfeng Li Xiujing Yin Xiujing Yin Huahua Yu Huahua Yu Pengcheng Li Pengcheng Li Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai
description	Scyphozoan Nemopilema nomurai envenomation is an unresolved threat to human health in Asian waters. Nemopilema nomurai venom metalloproteinases show important toxicities in skin damage and inflammation, but there is still no purified protein for further studies. In this study, high proteinase activity fractions in tentacle autolysis were isolated by ammonium sulfate precipitation, DEAE Sepharose Fast Flow, and Superdex 75 chromatography successively. Purification was guided by azocasein hydrolysis activity and SDS-PAGE. The final products were analyzed by LC-MS/MS. Four elution peaks purified by Superdex 75 chromatography had multiple protein bands but did not show proteinase activity. These fractions would recover proteinase activity after mixing again. Regulation mechanisms were speculated as binding metalloproteinase regulator or disaggregating metalloproteinase inhibitor by LC-MS/MS analysis. For the first time, a synergistic effect in N. nomurai proteinase activity was found in the purification process.
format	article
author	Chunlin Yu Chunlin Yu Rongfeng Li Rongfeng Li Xiujing Yin Xiujing Yin Huahua Yu Huahua Yu Pengcheng Li Pengcheng Li
author_facet	Chunlin Yu Chunlin Yu Rongfeng Li Rongfeng Li Xiujing Yin Xiujing Yin Huahua Yu Huahua Yu Pengcheng Li Pengcheng Li
author_sort	Chunlin Yu
title	Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai
title_short	Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai
title_full	Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai
title_fullStr	Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai
title_full_unstemmed	Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai
title_sort	synergistic effect of proteinase activity by purification and identification of toxic protease from nemopilema nomurai
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/74df05ad92184c84a95cef9cbbd819c9
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_version_	1718405910409248768

Synergistic Effect of Proteinase Activity by Purification and Identification of Toxic Protease From Nemopilema nomurai

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