Isolation of Mitochondria From Fresh Mice Lung Tissue

Direct analysis of isolated mitochondria enables a better understanding of lung dysfunction. Despite well-defined mitochondrial isolation protocols applicable to other tissues, such as the brain, kidney, heart, and liver, a robust and reproductive protocol has not yet been advanced for the lung. We...

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Autores principales:	Dayene de Assis Fernandes Caldeira, Dahienne Ferreira de Oliveira, João Paulo Cavalcanti-de-Albuquerque, Jose Hamilton Matheus Nascimento, Walter Araujo Zin, Leonardo Maciel
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lung mitochondria isolation O2-consumption ROS ATP mitochondrial assessment Physiology QP1-981
Acceso en línea:	https://doaj.org/article/74e135f5f16d47b2b69a63df5d0aa41c
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spelling	oai:doaj.org-article:74e135f5f16d47b2b69a63df5d0aa41c2021-12-01T19:31:20ZIsolation of Mitochondria From Fresh Mice Lung Tissue1664-042X10.3389/fphys.2021.748261https://doaj.org/article/74e135f5f16d47b2b69a63df5d0aa41c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.748261/fullhttps://doaj.org/toc/1664-042XDirect analysis of isolated mitochondria enables a better understanding of lung dysfunction. Despite well-defined mitochondrial isolation protocols applicable to other tissues, such as the brain, kidney, heart, and liver, a robust and reproductive protocol has not yet been advanced for the lung. We describe a protocol for the isolation of mitochondria from lung tissue aiming for functional analyses of mitochondrial O2 consumption, transmembrane potential, reactive oxygen species (ROS) formation, ATP production, and swelling. We compared our protocol to that used for heart mitochondrial function that is well-established in the literature, and achieved similar results.Dayene de Assis Fernandes CaldeiraDahienne Ferreira de OliveiraJoão Paulo Cavalcanti-de-AlbuquerqueJose Hamilton Matheus NascimentoWalter Araujo ZinLeonardo MacielLeonardo MacielFrontiers Media S.A.articlelung mitochondria isolationO2-consumptionROSATPmitochondrial assessmentPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung mitochondria isolation O2-consumption ROS ATP mitochondrial assessment Physiology QP1-981
spellingShingle	lung mitochondria isolation O2-consumption ROS ATP mitochondrial assessment Physiology QP1-981 Dayene de Assis Fernandes Caldeira Dahienne Ferreira de Oliveira João Paulo Cavalcanti-de-Albuquerque Jose Hamilton Matheus Nascimento Walter Araujo Zin Leonardo Maciel Leonardo Maciel Isolation of Mitochondria From Fresh Mice Lung Tissue
description	Direct analysis of isolated mitochondria enables a better understanding of lung dysfunction. Despite well-defined mitochondrial isolation protocols applicable to other tissues, such as the brain, kidney, heart, and liver, a robust and reproductive protocol has not yet been advanced for the lung. We describe a protocol for the isolation of mitochondria from lung tissue aiming for functional analyses of mitochondrial O2 consumption, transmembrane potential, reactive oxygen species (ROS) formation, ATP production, and swelling. We compared our protocol to that used for heart mitochondrial function that is well-established in the literature, and achieved similar results.
format	article
author	Dayene de Assis Fernandes Caldeira Dahienne Ferreira de Oliveira João Paulo Cavalcanti-de-Albuquerque Jose Hamilton Matheus Nascimento Walter Araujo Zin Leonardo Maciel Leonardo Maciel
author_facet	Dayene de Assis Fernandes Caldeira Dahienne Ferreira de Oliveira João Paulo Cavalcanti-de-Albuquerque Jose Hamilton Matheus Nascimento Walter Araujo Zin Leonardo Maciel Leonardo Maciel
author_sort	Dayene de Assis Fernandes Caldeira
title	Isolation of Mitochondria From Fresh Mice Lung Tissue
title_short	Isolation of Mitochondria From Fresh Mice Lung Tissue
title_full	Isolation of Mitochondria From Fresh Mice Lung Tissue
title_fullStr	Isolation of Mitochondria From Fresh Mice Lung Tissue
title_full_unstemmed	Isolation of Mitochondria From Fresh Mice Lung Tissue
title_sort	isolation of mitochondria from fresh mice lung tissue
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/74e135f5f16d47b2b69a63df5d0aa41c
work_keys_str_mv	AT dayenedeassisfernandescaldeira isolationofmitochondriafromfreshmicelungtissue AT dahienneferreiradeoliveira isolationofmitochondriafromfreshmicelungtissue AT joaopaulocavalcantidealbuquerque isolationofmitochondriafromfreshmicelungtissue AT josehamiltonmatheusnascimento isolationofmitochondriafromfreshmicelungtissue AT walteraraujozin isolationofmitochondriafromfreshmicelungtissue AT leonardomaciel isolationofmitochondriafromfreshmicelungtissue AT leonardomaciel isolationofmitochondriafromfreshmicelungtissue
_version_	1718404638112219136

Isolation of Mitochondria From Fresh Mice Lung Tissue

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