Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth

ABSTRACT N ε-Lysine acetylation has been recognized as a ubiquitous regulatory posttranslational modification that influences a variety of important biological processes in eukaryotic cells. Recently, it has been realized that acetylation is also prevalent in bacteria. Bacteria contain hundreds of a...

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Autores principales:	Valerie J. Carabetta, Todd M. Greco, Andrew W. Tanner, Ileana M. Cristea, David Dubnau
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	lysine acetylation MreB acetylome cell wall synthesis label-free quantification mass spectrometry Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/74eca4660f7b45558edcefbad696ba60
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spelling	oai:doaj.org-article:74eca4660f7b45558edcefbad696ba602021-12-02T18:39:34ZTemporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth10.1128/mSystems.00005-162379-5077https://doaj.org/article/74eca4660f7b45558edcefbad696ba602016-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00005-16https://doaj.org/toc/2379-5077ABSTRACT N ε-Lysine acetylation has been recognized as a ubiquitous regulatory posttranslational modification that influences a variety of important biological processes in eukaryotic cells. Recently, it has been realized that acetylation is also prevalent in bacteria. Bacteria contain hundreds of acetylated proteins, with functions affecting diverse cellular pathways. Still, little is known about the regulation or biological relevance of nearly all of these modifications. Here we characterize the cellular growth-associated regulation of the Bacillus subtilis acetylome. Using acetylation enrichment and quantitative mass spectrometry, we investigate the logarithmic and stationary growth phases, identifying over 2,300 unique acetylation sites on proteins that function in essential cellular pathways. We determine an acetylation motif, EK(ac)(D/Y/E), which resembles the eukaryotic mitochondrial acetylation signature, and a distinct stationary-phase-enriched motif. By comparing the changes in acetylation with protein abundances, we discover a subset of critical acetylation events that are temporally regulated during cell growth. We functionally characterize the stationary-phase-enriched acetylation on the essential shape-determining protein MreB. Using bioinformatics, mutational analysis, and fluorescence microscopy, we define a potential role for the temporal acetylation of MreB in restricting cell wall growth and cell diameter. IMPORTANCE The past decade highlighted Nε-lysine acetylation as a prevalent posttranslational modification in bacteria. However, knowledge regarding the physiological importance and temporal regulation of acetylation has remained limited. To uncover potential regulatory roles for acetylation, we analyzed how acetylation patterns and abundances change between growth phases in B. subtilis. To demonstrate that the identification of cell growth-dependent modifications can point to critical regulatory acetylation events, we further characterized MreB, the cell shape-determining protein. Our findings led us to propose a role for MreB acetylation in controlling cell width by restricting cell wall growth.Valerie J. CarabettaTodd M. GrecoAndrew W. TannerIleana M. CristeaDavid DubnauAmerican Society for Microbiologyarticlelysine acetylationMreBacetylomecell wall synthesislabel-free quantificationmass spectrometryMicrobiologyQR1-502ENmSystems, Vol 1, Iss 3 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	lysine acetylation MreB acetylome cell wall synthesis label-free quantification mass spectrometry Microbiology QR1-502
spellingShingle	lysine acetylation MreB acetylome cell wall synthesis label-free quantification mass spectrometry Microbiology QR1-502 Valerie J. Carabetta Todd M. Greco Andrew W. Tanner Ileana M. Cristea David Dubnau Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
description	ABSTRACT N ε-Lysine acetylation has been recognized as a ubiquitous regulatory posttranslational modification that influences a variety of important biological processes in eukaryotic cells. Recently, it has been realized that acetylation is also prevalent in bacteria. Bacteria contain hundreds of acetylated proteins, with functions affecting diverse cellular pathways. Still, little is known about the regulation or biological relevance of nearly all of these modifications. Here we characterize the cellular growth-associated regulation of the Bacillus subtilis acetylome. Using acetylation enrichment and quantitative mass spectrometry, we investigate the logarithmic and stationary growth phases, identifying over 2,300 unique acetylation sites on proteins that function in essential cellular pathways. We determine an acetylation motif, EK(ac)(D/Y/E), which resembles the eukaryotic mitochondrial acetylation signature, and a distinct stationary-phase-enriched motif. By comparing the changes in acetylation with protein abundances, we discover a subset of critical acetylation events that are temporally regulated during cell growth. We functionally characterize the stationary-phase-enriched acetylation on the essential shape-determining protein MreB. Using bioinformatics, mutational analysis, and fluorescence microscopy, we define a potential role for the temporal acetylation of MreB in restricting cell wall growth and cell diameter. IMPORTANCE The past decade highlighted Nε-lysine acetylation as a prevalent posttranslational modification in bacteria. However, knowledge regarding the physiological importance and temporal regulation of acetylation has remained limited. To uncover potential regulatory roles for acetylation, we analyzed how acetylation patterns and abundances change between growth phases in B. subtilis. To demonstrate that the identification of cell growth-dependent modifications can point to critical regulatory acetylation events, we further characterized MreB, the cell shape-determining protein. Our findings led us to propose a role for MreB acetylation in controlling cell width by restricting cell wall growth.
format	article
author	Valerie J. Carabetta Todd M. Greco Andrew W. Tanner Ileana M. Cristea David Dubnau
author_facet	Valerie J. Carabetta Todd M. Greco Andrew W. Tanner Ileana M. Cristea David Dubnau
author_sort	Valerie J. Carabetta
title	Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_short	Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_full	Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_fullStr	Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_full_unstemmed	Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth
title_sort	temporal regulation of the <named-content content-type="genus-species">bacillus subtilis</named-content> acetylome and evidence for a role of mreb acetylation in cell wall growth
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/74eca4660f7b45558edcefbad696ba60
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Temporal Regulation of the <named-content content-type="genus-species">Bacillus subtilis</named-content> Acetylome and Evidence for a Role of MreB Acetylation in Cell Wall Growth

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