Programmable DNA looping using engineered bivalent dCas9 complexes

DNA loops are a ubiquitious feature of gene regulation across the kingdoms of life. Here the authors design a Cas9-based dimerization system for inducing DNA loops in E. coli, allowing activation and rewiring of gene expression.

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Detalles Bibliográficos
Autores principales: Nan Hao, Keith E. Shearwin, Ian B. Dodd
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/74f9626a97ce451fafaa5aa6b162cad8
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Descripción
Sumario:DNA loops are a ubiquitious feature of gene regulation across the kingdoms of life. Here the authors design a Cas9-based dimerization system for inducing DNA loops in E. coli, allowing activation and rewiring of gene expression.