Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis

Introduction: Multiple Sclerosis (MS) is the chronic inflammation of the Central Nervous System (CNS) and autoimmune disease. MS is most widely considered to be mediated by the activation of myelin-specific T CD4+ cells as well as TH1 and TH17 cells. TH17 cells are involved in the pathogenesis of MS...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Boshra Afshar, Mazdak Ganjalikhani-Hakemi, Zahra Khalifezadeh Esfahani, Nahid Eskandari, Vahid Shaygannajad, Fahimeh Hosseininasab, Freshteh Alsahebfosoul
Formato:	article
Lenguaje:	EN
Publicado:	Iran University of Medical Sciences 2021
Materias:	multiple sclerosis epigallocatechin-3-gallate (egcg) rorc2 hypoxia-inducible factor 1-alpha (hif-1α) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/74fea0b35a0749168319f907ec2f5073
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:74fea0b35a0749168319f907ec2f5073
record_format	dspace
spelling	oai:doaj.org-article:74fea0b35a0749168319f907ec2f50732021-12-05T08:43:44ZEvaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis2008-126X2228-7442https://doaj.org/article/74fea0b35a0749168319f907ec2f50732021-07-01T00:00:00Zhttp://bcn.iums.ac.ir/article-1-1667-en.htmlhttps://doaj.org/toc/2008-126Xhttps://doaj.org/toc/2228-7442Introduction: Multiple Sclerosis (MS) is the chronic inflammation of the Central Nervous System (CNS) and autoimmune disease. MS is most widely considered to be mediated by the activation of myelin-specific T CD4+ cells as well as TH1 and TH17 cells. TH17 cells are involved in the pathogenesis of MS in various manners. HIF-1α and RORC are required for the natural differentiation of TH17; they are essential transcription factors for the evolution of TH17 cells. Numerous studies indicated that Epigallocatechin Gallate (EGCG) presents immunomodulatory and anti-inflammatory effects. This study investigated the effects of EGCG on normoxic HIF-1α and RORC2 expression in PBMCs among MS patients. Methods: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from the whole blood of new cases of MS. The cells were cultured in the presence of a different concentration of EGCG (25, 50,100μM) for 18 and 48 hours. Next, HIF-1α and RORC2 level expressions were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time PCR, respectively. Results: The results showed that EGCG significantly decreased RORC2 gene expression. EGCG did not affect the level of HIF-1α. Conclusion: However, EGCG did not influence the level of HIF-1α. Our present data has led us to conclude that EGCG could be considered as an anti-inflammatory agent may serve as an achievable therapeutic agent for MS.Boshra AfsharMazdak Ganjalikhani-HakemiZahra Khalifezadeh EsfahaniNahid EskandariVahid ShaygannajadFahimeh HosseininasabFreshteh AlsahebfosoulIran University of Medical Sciencesarticlemultiple sclerosisepigallocatechin-3-gallate (egcg)rorc2hypoxia-inducible factor 1-alpha (hif-1α)Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBasic and Clinical Neuroscience, Vol 12, Iss 4, Pp 533-540 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multiple sclerosis epigallocatechin-3-gallate (egcg) rorc2 hypoxia-inducible factor 1-alpha (hif-1α) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	multiple sclerosis epigallocatechin-3-gallate (egcg) rorc2 hypoxia-inducible factor 1-alpha (hif-1α) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Boshra Afshar Mazdak Ganjalikhani-Hakemi Zahra Khalifezadeh Esfahani Nahid Eskandari Vahid Shaygannajad Fahimeh Hosseininasab Freshteh Alsahebfosoul Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis
description	Introduction: Multiple Sclerosis (MS) is the chronic inflammation of the Central Nervous System (CNS) and autoimmune disease. MS is most widely considered to be mediated by the activation of myelin-specific T CD4+ cells as well as TH1 and TH17 cells. TH17 cells are involved in the pathogenesis of MS in various manners. HIF-1α and RORC are required for the natural differentiation of TH17; they are essential transcription factors for the evolution of TH17 cells. Numerous studies indicated that Epigallocatechin Gallate (EGCG) presents immunomodulatory and anti-inflammatory effects. This study investigated the effects of EGCG on normoxic HIF-1α and RORC2 expression in PBMCs among MS patients. Methods: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from the whole blood of new cases of MS. The cells were cultured in the presence of a different concentration of EGCG (25, 50,100μM) for 18 and 48 hours. Next, HIF-1α and RORC2 level expressions were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time PCR, respectively. Results: The results showed that EGCG significantly decreased RORC2 gene expression. EGCG did not affect the level of HIF-1α. Conclusion: However, EGCG did not influence the level of HIF-1α. Our present data has led us to conclude that EGCG could be considered as an anti-inflammatory agent may serve as an achievable therapeutic agent for MS.
format	article
author	Boshra Afshar Mazdak Ganjalikhani-Hakemi Zahra Khalifezadeh Esfahani Nahid Eskandari Vahid Shaygannajad Fahimeh Hosseininasab Freshteh Alsahebfosoul
author_facet	Boshra Afshar Mazdak Ganjalikhani-Hakemi Zahra Khalifezadeh Esfahani Nahid Eskandari Vahid Shaygannajad Fahimeh Hosseininasab Freshteh Alsahebfosoul
author_sort	Boshra Afshar
title	Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis
title_short	Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis
title_full	Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis
title_fullStr	Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis
title_full_unstemmed	Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis
title_sort	evaluating the effects of epigallocatechin-3-gallate on hif-1α protein and rorc gene expression in peripheral blood mononuclear cells in patients with multiple sclerosis
publisher	Iran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/74fea0b35a0749168319f907ec2f5073
work_keys_str_mv	AT boshraafshar evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis AT mazdakganjalikhanihakemi evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis AT zahrakhalifezadehesfahani evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis AT nahideskandari evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis AT vahidshaygannajad evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis AT fahimehhosseininasab evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis AT freshtehalsahebfosoul evaluatingtheeffectsofepigallocatechin3gallateonhif1aproteinandrorcgeneexpressioninperipheralbloodmononuclearcellsinpatientswithmultiplesclerosis
_version_	1718372563408650240

Evaluating the Effects of Epigallocatechin-3-Gallate on HIF-1α Protein and RORC Gene Expression in Peripheral Blood Mononuclear Cells in Patients With Multiple Sclerosis

Ejemplares similares