Exploring the Diversity of the Human Blood Virome

Metagenomics is greatly improving our ability to discover new viruses, as well as their possible associations with disease. However, metagenomics has also changed our understanding of viruses in general. The vast expansion of currently known viral diversity has revealed a large fraction of non-patho...

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Autores principales:	María Cebriá-Mendoza, María A. Bracho, Cristina Arbona, Luís Larrea, Wladimiro Díaz, Rafael Sanjuán, José M. Cuevas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	orphan virus blood virome anellovirus pegivirus virus discovery metagenomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/75010e9e533e42cd9c181b4a4a70834c
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spelling	oai:doaj.org-article:75010e9e533e42cd9c181b4a4a70834c2021-11-25T19:14:34ZExploring the Diversity of the Human Blood Virome10.3390/v131123221999-4915https://doaj.org/article/75010e9e533e42cd9c181b4a4a70834c2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2322https://doaj.org/toc/1999-4915Metagenomics is greatly improving our ability to discover new viruses, as well as their possible associations with disease. However, metagenomics has also changed our understanding of viruses in general. The vast expansion of currently known viral diversity has revealed a large fraction of non-pathogenic viruses, and offers a new perspective in which viruses function as important components of many ecosystems. In this vein, studies of the human blood virome are often motivated by the search for new viral diseases, especially those associated with blood transfusions. However, these studies have revealed the common presence of apparently non-pathogenic viruses in blood, particularly human anelloviruses and, to a lower extent, human pegiviruses (HPgV). To shed light on the diversity of the human blood virome, we subjected pooled plasma samples from 587 healthy donors in Spain to a viral enrichment protocol, followed by massive parallel sequencing. This showed that anelloviruses were clearly the major component of the blood virome and showed remarkable diversity. In total, we assembled 332 complete or near-complete anellovirus genomes, 50 of which could be considered new species. HPgV was much less frequent, but we, nevertheless, recovered 17 different isolates that we subsequently used for characterizing the diversity of this virus. In-depth investigation of the human blood virome should help to elucidate the ecology of these viruses, and to unveil potentially associated diseases.María Cebriá-MendozaMaría A. BrachoCristina ArbonaLuís LarreaWladimiro DíazRafael SanjuánJosé M. CuevasMDPI AGarticleorphan virusblood viromeanelloviruspegivirusvirus discoverymetagenomicsMicrobiologyQR1-502ENViruses, Vol 13, Iss 2322, p 2322 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	orphan virus blood virome anellovirus pegivirus virus discovery metagenomics Microbiology QR1-502
spellingShingle	orphan virus blood virome anellovirus pegivirus virus discovery metagenomics Microbiology QR1-502 María Cebriá-Mendoza María A. Bracho Cristina Arbona Luís Larrea Wladimiro Díaz Rafael Sanjuán José M. Cuevas Exploring the Diversity of the Human Blood Virome
description	Metagenomics is greatly improving our ability to discover new viruses, as well as their possible associations with disease. However, metagenomics has also changed our understanding of viruses in general. The vast expansion of currently known viral diversity has revealed a large fraction of non-pathogenic viruses, and offers a new perspective in which viruses function as important components of many ecosystems. In this vein, studies of the human blood virome are often motivated by the search for new viral diseases, especially those associated with blood transfusions. However, these studies have revealed the common presence of apparently non-pathogenic viruses in blood, particularly human anelloviruses and, to a lower extent, human pegiviruses (HPgV). To shed light on the diversity of the human blood virome, we subjected pooled plasma samples from 587 healthy donors in Spain to a viral enrichment protocol, followed by massive parallel sequencing. This showed that anelloviruses were clearly the major component of the blood virome and showed remarkable diversity. In total, we assembled 332 complete or near-complete anellovirus genomes, 50 of which could be considered new species. HPgV was much less frequent, but we, nevertheless, recovered 17 different isolates that we subsequently used for characterizing the diversity of this virus. In-depth investigation of the human blood virome should help to elucidate the ecology of these viruses, and to unveil potentially associated diseases.
format	article
author	María Cebriá-Mendoza María A. Bracho Cristina Arbona Luís Larrea Wladimiro Díaz Rafael Sanjuán José M. Cuevas
author_facet	María Cebriá-Mendoza María A. Bracho Cristina Arbona Luís Larrea Wladimiro Díaz Rafael Sanjuán José M. Cuevas
author_sort	María Cebriá-Mendoza
title	Exploring the Diversity of the Human Blood Virome
title_short	Exploring the Diversity of the Human Blood Virome
title_full	Exploring the Diversity of the Human Blood Virome
title_fullStr	Exploring the Diversity of the Human Blood Virome
title_full_unstemmed	Exploring the Diversity of the Human Blood Virome
title_sort	exploring the diversity of the human blood virome
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/75010e9e533e42cd9c181b4a4a70834c
work_keys_str_mv	AT mariacebriamendoza exploringthediversityofthehumanbloodvirome AT mariaabracho exploringthediversityofthehumanbloodvirome AT cristinaarbona exploringthediversityofthehumanbloodvirome AT luislarrea exploringthediversityofthehumanbloodvirome AT wladimirodiaz exploringthediversityofthehumanbloodvirome AT rafaelsanjuan exploringthediversityofthehumanbloodvirome AT josemcuevas exploringthediversityofthehumanbloodvirome
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Exploring the Diversity of the Human Blood Virome

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