Twisting of DNA Origami from Intercalators

Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-h...

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Autores principales:	Reza M. Zadegan, Elias G. Lindau, William P. Klein, Christopher Green, Elton Graugnard, Bernard Yurke, Wan Kuang, William L. Hughes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/75033a4fb23149ec99bcb5574ed145ab
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spelling	oai:doaj.org-article:75033a4fb23149ec99bcb5574ed145ab2021-12-02T16:06:30ZTwisting of DNA Origami from Intercalators10.1038/s41598-017-07796-32045-2322https://doaj.org/article/75033a4fb23149ec99bcb5574ed145ab2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07796-3https://doaj.org/toc/2045-2322Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-helix bundles. Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators. Our findings demonstrate that DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentration dependent, and (ii) twisting saturates at elevated concentrations. This study provides insight into how complex DNA structures undergo conformational changes when exposed to intercalators and may be of relevance when exploring how intercalating drugs interact with condensed biological structures such as chromatin and chromosomes, as well as chromatin analogous gene expression devices.Reza M. ZadeganElias G. LindauWilliam P. KleinChristopher GreenElton GraugnardBernard YurkeWan KuangWilliam L. HughesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reza M. Zadegan Elias G. Lindau William P. Klein Christopher Green Elton Graugnard Bernard Yurke Wan Kuang William L. Hughes Twisting of DNA Origami from Intercalators
description	Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-helix bundles. Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators. Our findings demonstrate that DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentration dependent, and (ii) twisting saturates at elevated concentrations. This study provides insight into how complex DNA structures undergo conformational changes when exposed to intercalators and may be of relevance when exploring how intercalating drugs interact with condensed biological structures such as chromatin and chromosomes, as well as chromatin analogous gene expression devices.
format	article
author	Reza M. Zadegan Elias G. Lindau William P. Klein Christopher Green Elton Graugnard Bernard Yurke Wan Kuang William L. Hughes
author_facet	Reza M. Zadegan Elias G. Lindau William P. Klein Christopher Green Elton Graugnard Bernard Yurke Wan Kuang William L. Hughes
author_sort	Reza M. Zadegan
title	Twisting of DNA Origami from Intercalators
title_short	Twisting of DNA Origami from Intercalators
title_full	Twisting of DNA Origami from Intercalators
title_fullStr	Twisting of DNA Origami from Intercalators
title_full_unstemmed	Twisting of DNA Origami from Intercalators
title_sort	twisting of dna origami from intercalators
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/75033a4fb23149ec99bcb5574ed145ab
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_version_	1718384968757936128

Twisting of DNA Origami from Intercalators

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