Twisting of DNA Origami from Intercalators

Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-h...

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Autores principales: Reza M. Zadegan, Elias G. Lindau, William P. Klein, Christopher Green, Elton Graugnard, Bernard Yurke, Wan Kuang, William L. Hughes
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/75033a4fb23149ec99bcb5574ed145ab
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spelling oai:doaj.org-article:75033a4fb23149ec99bcb5574ed145ab2021-12-02T16:06:30ZTwisting of DNA Origami from Intercalators10.1038/s41598-017-07796-32045-2322https://doaj.org/article/75033a4fb23149ec99bcb5574ed145ab2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07796-3https://doaj.org/toc/2045-2322Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-helix bundles. Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators. Our findings demonstrate that DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentration dependent, and (ii) twisting saturates at elevated concentrations. This study provides insight into how complex DNA structures undergo conformational changes when exposed to intercalators and may be of relevance when exploring how intercalating drugs interact with condensed biological structures such as chromatin and chromosomes, as well as chromatin analogous gene expression devices.Reza M. ZadeganElias G. LindauWilliam P. KleinChristopher GreenElton GraugnardBernard YurkeWan KuangWilliam L. HughesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-5 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Reza M. Zadegan
Elias G. Lindau
William P. Klein
Christopher Green
Elton Graugnard
Bernard Yurke
Wan Kuang
William L. Hughes
Twisting of DNA Origami from Intercalators
description Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-helix bundles. Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators. Our findings demonstrate that DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentration dependent, and (ii) twisting saturates at elevated concentrations. This study provides insight into how complex DNA structures undergo conformational changes when exposed to intercalators and may be of relevance when exploring how intercalating drugs interact with condensed biological structures such as chromatin and chromosomes, as well as chromatin analogous gene expression devices.
format article
author Reza M. Zadegan
Elias G. Lindau
William P. Klein
Christopher Green
Elton Graugnard
Bernard Yurke
Wan Kuang
William L. Hughes
author_facet Reza M. Zadegan
Elias G. Lindau
William P. Klein
Christopher Green
Elton Graugnard
Bernard Yurke
Wan Kuang
William L. Hughes
author_sort Reza M. Zadegan
title Twisting of DNA Origami from Intercalators
title_short Twisting of DNA Origami from Intercalators
title_full Twisting of DNA Origami from Intercalators
title_fullStr Twisting of DNA Origami from Intercalators
title_full_unstemmed Twisting of DNA Origami from Intercalators
title_sort twisting of dna origami from intercalators
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/75033a4fb23149ec99bcb5574ed145ab
work_keys_str_mv AT rezamzadegan twistingofdnaorigamifromintercalators
AT eliasglindau twistingofdnaorigamifromintercalators
AT williampklein twistingofdnaorigamifromintercalators
AT christophergreen twistingofdnaorigamifromintercalators
AT eltongraugnard twistingofdnaorigamifromintercalators
AT bernardyurke twistingofdnaorigamifromintercalators
AT wankuang twistingofdnaorigamifromintercalators
AT williamlhughes twistingofdnaorigamifromintercalators
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