Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing

Plant-associated fungi, or the mycobiome, inhabit plant surfaces above ground, reside in plant tissues as endophytes, or are rhizosphere in the narrow zone of soil surrounding plant roots. Studies have characterized mycobiomes of various plant species, but little is known about the sorghum mycobiome...

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Autores principales:	Gilmore T. Pambuka, Tonjock Rosemary Kinge, Soumya Ghosh, Errol D. Cason, Martin M. Nyaga, Marieka Gryzenhout
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sorghum cultivars plant tissues substrates above ground below ground Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/75062fcd74224e38b02ba1380b003cc1
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spelling	oai:doaj.org-article:75062fcd74224e38b02ba1380b003cc12021-11-25T18:06:27ZBaseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing10.3390/jof71109782309-608Xhttps://doaj.org/article/75062fcd74224e38b02ba1380b003cc12021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/978https://doaj.org/toc/2309-608XPlant-associated fungi, or the mycobiome, inhabit plant surfaces above ground, reside in plant tissues as endophytes, or are rhizosphere in the narrow zone of soil surrounding plant roots. Studies have characterized mycobiomes of various plant species, but little is known about the sorghum mycobiome, especially in Africa, despite sorghum being one of the most important indigenous and commercial cereals in Africa. In this study, the mycobiome associated with above- and below-ground tissues of three commercial sorghum cultivars, as well as from rhizosphere and surrounding bulk soil samples, were sequenced using targeted sequencing with the Illumina MiSeq platform. Relative abundance differences between fungal communities were found between above-ground and below-ground niches, with most differences mostly in the dominant MOTUs, such as Davidiellaceae sp. (<i>Cladosporium</i>), Didymellaceae sp. 1 (<i>Phoma</i>), <i>Fusarium</i>, <i>Cryptococcus</i> and <i>Mucor</i>. Above-ground communities also appeared to be more diverse than below-ground communities, and plants harboured the most diversity. A considerable number of MOTUs were shared between the cultivars although, especially for NS5511, their abundances often differed. Several of the detected fungal groups include species that are plant pathogens of sorghum, such as <i>Fusarium</i>, and, at low levels, <i>Alternaria</i> and the Ustilaginomycetes. Findings from this study illustrate the usefulness of targeted sequencing of the ITS rDNA gene region (ITS2) to survey and monitor sorghum fungal communities and those from associated soils. This knowledge may provide tools for disease management and crop production and improvement.Gilmore T. PambukaTonjock Rosemary KingeSoumya GhoshErrol D. CasonMartin M. NyagaMarieka GryzenhoutMDPI AGarticlesorghumcultivarsplant tissuessubstratesabove groundbelow groundBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 978, p 978 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sorghum cultivars plant tissues substrates above ground below ground Biology (General) QH301-705.5
spellingShingle	sorghum cultivars plant tissues substrates above ground below ground Biology (General) QH301-705.5 Gilmore T. Pambuka Tonjock Rosemary Kinge Soumya Ghosh Errol D. Cason Martin M. Nyaga Marieka Gryzenhout Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing
description	Plant-associated fungi, or the mycobiome, inhabit plant surfaces above ground, reside in plant tissues as endophytes, or are rhizosphere in the narrow zone of soil surrounding plant roots. Studies have characterized mycobiomes of various plant species, but little is known about the sorghum mycobiome, especially in Africa, despite sorghum being one of the most important indigenous and commercial cereals in Africa. In this study, the mycobiome associated with above- and below-ground tissues of three commercial sorghum cultivars, as well as from rhizosphere and surrounding bulk soil samples, were sequenced using targeted sequencing with the Illumina MiSeq platform. Relative abundance differences between fungal communities were found between above-ground and below-ground niches, with most differences mostly in the dominant MOTUs, such as Davidiellaceae sp. (<i>Cladosporium</i>), Didymellaceae sp. 1 (<i>Phoma</i>), <i>Fusarium</i>, <i>Cryptococcus</i> and <i>Mucor</i>. Above-ground communities also appeared to be more diverse than below-ground communities, and plants harboured the most diversity. A considerable number of MOTUs were shared between the cultivars although, especially for NS5511, their abundances often differed. Several of the detected fungal groups include species that are plant pathogens of sorghum, such as <i>Fusarium</i>, and, at low levels, <i>Alternaria</i> and the Ustilaginomycetes. Findings from this study illustrate the usefulness of targeted sequencing of the ITS rDNA gene region (ITS2) to survey and monitor sorghum fungal communities and those from associated soils. This knowledge may provide tools for disease management and crop production and improvement.
format	article
author	Gilmore T. Pambuka Tonjock Rosemary Kinge Soumya Ghosh Errol D. Cason Martin M. Nyaga Marieka Gryzenhout
author_facet	Gilmore T. Pambuka Tonjock Rosemary Kinge Soumya Ghosh Errol D. Cason Martin M. Nyaga Marieka Gryzenhout
author_sort	Gilmore T. Pambuka
title	Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing
title_short	Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing
title_full	Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing
title_fullStr	Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing
title_full_unstemmed	Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing
title_sort	baseline data of the fungal phytobiome of three sorghum (<i>sorghum bicolor</i>) cultivars in south africa using targeted environmental sequencing
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/75062fcd74224e38b02ba1380b003cc1
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Baseline Data of the Fungal Phytobiome of Three Sorghum (<i>Sorghum bicolor</i>) Cultivars in South Africa using Targeted Environmental Sequencing

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